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1.
2.
OBJECTIVE: To determine prevalence of various pheno- and genotypes of Serpulina sp in young pigs in relation to diarrhea and feed medication in Swedish pig-rearing herds. DESIGN: Isolation of spirochetes. Phenotypical and genotypical classification. SAMPLE POPULATION: Young pigs (n = 358) in 19 pigrearing herds. PROCEDURE: Serpulina isolates were classified according to a biochemical scheme based on hemolysis, indole production, hippurate hydrolysis, and alpha-galactosidase, alpha-glucosidase, and beta-glucosidase activities. The 16S rRNA sequences for 10 of the field strains and 2 type strains of Serpulina spp were aligned and compared. Minimum inhibitory concentrations of olaquindox for 9 of the strains were determined. RESULTS: Weakly beta-hemolytic intestinal spirochetes (WBHIS) were isolated from 17 of the herds and 65% of the samples. More than 1 phenotype of WBHIS was found in 12 of the 19 herds. S hyodysenteriae was not isolated in any of the herds. Hippurate-positive WBHIS were isolated in 6 of 7 herds affected by diarrhea, but in only 1 of 8 herds without diarrhea. Hippurate-positive strains were closely related to the pathogenic strain P43 if judged from sequence comparisons. Strains with the same biochemical profile isolated within a herd had identical sequences, but when isolated from different herds, sequence differences were observed. The prevalence of WBHIS was reduced in herds medicated with olaquindox. Investigated field strains had minimum inhibitory concentration values < or = 1 microgram/ml for olaquindox. CONCLUSION: The presence of WBHIS, with the ability to hydrolyze hippurate, was related to diarrhea in pig herds. CLINICAL RELEVANCE: Potentially pathogenic WBHIS can be distinguished from nonpathogenic strains by the hippurate hydrolysis test. 相似文献
3.
For viruses made of nucleic acid and protein, the structure of the protein outer shell has, in the past, been found to be uniquely determined by the viral genome. However, here, non-denaturing agarose gel electrophoresis of bacteriophage T7 reveals two states of the mature T7 capsid; the conditions of growth are found to alter the population by T7 of these two electrophoretically defined states. Both states have been previously observed for a genetically altered T7 and they are observed here for wild-type T7. The average electrical surface charge density of a bacteriophage particle (delta) determines its state; the delta of particles in both states is negative. For a given condition of growth, the population of these two states is influenced by the extent to which the major T7 outer shell protein, p10A, is accompanied by its minor readthrough variant, p10B. Comparison of the two electrophoretic states reveals the following. (1) No difference in radius is present in the outer shell (+/-2%). (2) As the pH of electrophoresis is either increased or decreased from neutrality, the state becomes more highly populated for which delta is greater in magnitude (state 1). By changing the pH, some T7 particles are made to change state. (3) Particles in state 1 adsorb less quickly to host cells than do the particles in the alternative state (state 2). This latter observation suggests the hypothesis that state 1 evolved to reduce the probability of re-initiating an infection when conditions are not favorable for growth. This hypothesis is supported by the observation that, as conditions of growth become apparently more unfavorable, progeny increasingly populate state 1. 相似文献
4.
Comments that L. B. Silverstein's (see record 1992-05293-001) article on child care and maternal employment contributes unfounded and damaging misbeliefs regarding fathers. The dad-as-cad myth suggests intentional malevolence or at least blatant failure by fathers to care for others, and suggests that their failure to share equally in child care and household management is the primary cause of marital conflict. W. B. Johnson and D. Hayes propose a research agenda for fathering that originates in a coherent model of male development in the context of ever-changing environmental demands. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
5.
At higher operating frequencies, the field dependence of the carrier velocity in p-i-n photodetectors generates harmonics and intermodulation products that can degrade the dynamic range of RF fiber-optic links. The authors present both a perturbational theory and measured harmonic data for a p-i-n photodiode operated at very high power densities which show that this and other detector nonlinear effects need not seriously compromise link performance. In particular, neither transit-time nor static nonlinearities in p-i-n photodiodes need limit the dynamic range of fiber-optic links operating below 5 GHz. The fact that the theoretical bandwidth of the photodiode, with all parasitic capacitance and inductance ideally removed, is 17 GHz, suggests that comparable spur-free performance should be achievable at X and Ku -band frequencies, once packaging parasitics are reduced 相似文献
6.
Resurgence has been shown in human and nonhuman operant behavior, but not in derived relational responses. The present study examined this issue. Twenty-three undergraduates were trained to make conditional discriminations in a three-choice matching-to-sample paradigm. The training resulted in three equivalence classes, each consisting of four arbitrarily configured visual stimuli. The same 12 stimuli were then reorganized, and the conditional discrimination training was repeated such that three new classes were possible. In a subsequent test of derived relations, most subjects showed response patterns that were consistent with the altered conditional discriminations. Subjects were then exposed to conditional discrimination trials under extinction. Most subjects continued to respond consistently with the most recently reinforced conditional discrimination trials. During the next phase, subjects were exposed to symmetry and equivalence trials. Responses consistent with the most recent training produced feedback saying that the responses were incorrect, whereas other responses produced no feedback. Most subjects showed a resurgence of responding that was consistent with their earlier training. Finally, subjects were exposed to conditional discrimination trials carried out in extinction. Most subjects continued to show a resurgence of responding that was consistent with their early training. 相似文献
7.
Multitrait restricted maximum likelihood methods were used for estimation of heritabilities and genetic and phenotypic correlations between calving ease and calf survival. These analyses examined both direct and maternal effects. Genetic correlation for direct effects between calving ease and calf survival was approximately -.8 in both heifers and cows, indicating favorable associations, whereas phenotypic correlations were -.32 and -.22. 相似文献
8.
As far as flocculation is concerned, the agglomeration of suspended particles into flocs is highly linked to the hydraulic behaviour of the agitation. The Turbomix is a special design of mixing tank; its design was developed to better control the flow during the flocculation stage. It enables a significant decrease in footprint of the process. The combination of the Turbomix and ballasted flocculation has been studied during pilot trials in terms of treatment efficiency. Its controlled hydraulic behaviour explains the efficiency of the process as proved by CFD investigation. 相似文献
9.
Experimental studies on phase equilibria in the multicomponent system PbO-ZnO-CaO-SiO2-FeO-Fe2O3 in air have been conducted to characterize the phase relations of a complex slag system used in commercial lead oxidation
smelting. The liquidus in the pseudo-ternary section ZnO-“Fe2O3”-(PbO + CaO + SiO2) with the CaO/SiO2 weight ratio of 0.35 and the PbO/(CaO + SiO2) weight ratio of 5.0 has been constructed using results of over 100 high-temperature equilibration and quenching experiments
followed by electron probe X-ray microanalysis. The liquidus in this pseudoternary section contains primary phase fields of
spinel (zinc ferrite) Zn
x
Fe3−x
O4+y
, zincite Zn
u
Fe1−u
O, melilite Pb
v
Ca2−v
Zn
w
Fe1−w
Si2O7, hematite Fe2O3, magneto-plumbite PbFe10O16, and dicalcium silicate Ca2−t
Pb
t
SiO4. The laboratory results are compared with the slags obtained from an industrial reactor. 相似文献
10.
The processing of images obtained from satellites often involves highly repetitive calculations on very large amounts of data. This processing is extremely time consuming when these calculations are performed on sequential machines. Parallel computers are well suited to handling computationally expensive operations such as higher order interpolations on large data sets. This paper decribes work undertaken to develop parallel implementations of a set of resampling procedures on an Alliant VFX/4. Each resampling procedure implemented has been optimised in three stages. First, the algorithm has been restructured, where two-dimensional resampling is performed by two one-dimensional resampling operations. Second, each procedure has been reprogrammed in such a way that the autoparallelisation provided by the FX/Fortran compiler has been exploited. Thirdly, data dependent analysis of each procedure has been performed in order to achieve full optimization of each procedure; each procedure has been restructured where appropriate to circumvent vectorisation and concurrency inhibiting data dependencies. The nature and extent of the code optimization achieved for each procedure is presented in this paper. The original code for the most computationally expensive procedure, as targeted at a sequential machine, was found to have an execution time of 4900 seconds on the Alliant VFX/4 when compiled with regular compiler optimization options. Following algorithmic redesigning and reprogramming of the code, as indicated in stage 1 and stage 2, the execution time was reduced to 248 seconds. Restructuring of the code following data dependency analysis indicated in stage 3 in order to avoid data dependencies and allow concurrency and vectorisation, further reduced execution time to 162 seconds. The consequence of this work is that higher-order resampling methods which had not previous been practical are now routinely performed on the Alliant VFX/4 at the University of Dundee. 相似文献