Elevated Expression of CCN3 in Articular Cartilage Induces Osteoarthritis in Hip Joints Irrespective of Age and Weight Bearing

Osteoarthritis (OA) occurs not only in the knee but also in peripheral joints throughout the whole body. Previously, we have shown that the expression of cellular communication network factor 3 (CCN3), a matricellular protein, increases with age in knee articular cartilage, and the misexpression of CCN3 in cartilage induces senescence-associated secretory phenotype (SASP) factors, indicating that CCN3 promotes cartilage senescence. Here, we investigated the correlation between CCN3 expression and OA degenerative changes, principally in human femoral head cartilage. Human femoral heads obtained from patients who received total hip arthroplasty were categorized into OA and femoral neck fracture (normal) groups without significant age differences. Gene expression analysis of RNA obtained from femoral head cartilage revealed that CCN3 and MMP-13 expression in the non-weight-bearing part was significantly higher in the OA group than in the normal group, whereas the weight-bearing OA parts and normal cartilage showed no significant differences in the expression of these genes. The expression of COL10A1, however, was significantly higher in weight-bearing OA parts compared with normal weight-bearing parts, and was also higher in weight-bearing parts compared with non-weight-bearing parts in the OA group. In contrast, OA primary chondrocytes from weight-bearing parts showed higher expression of CCN3, p16, ADAMTS4, and IL-1β than chondrocytes from the corresponding normal group, and higher ADAMTS4 and IL-1β in the non-weight-bearing part compared with the corresponding normal group. Acan expression was significantly lower in the non-weight-bearing group in OA primary chondrocytes than in the corresponding normal chondrocytes. The expression level of CCN3 did not show significant differences between the weight-bearing part and non-weight-bearing part in both OA and normal primary chondrocytes. Immunohistochemical analysis showed accumulated CCN3 and aggrecan neoepitope staining in both the weight-bearing part and non-weight-bearing part in the OA group compared with the normal group. The CCN3 expression level in cartilage had a positive correlation with the Mankin score. X-ray analysis of cartilage-specific CCN3 overexpression mice (Tg) revealed deformation of the femoral and humeral head in the early stage, and immunohistochemical analysis showed accumulated aggrecan neoepitope staining as well as CCN3 staining and the roughening of the joint surface in Tg femoral and humeral heads. Primary chondrocytes from the Tg femoral head showed enhanced expression of Ccn3, Adamts5, p16, Il-6, and Tnfα, and decreased expression of Col2a1 and -an. These findings indicate a correlation between OA degenerative changes and the expression of CCN3, irrespective of age and mechanical loading. Furthermore, the Mankin score indicates that the expression level of Ccn3 correlates with the progression of OA.     

Hetero-stereocomplex formation of stereoblock copolymer of substituted and non-substituted poly(lactide)s

The crystallization behavior of the stereoblock copolymer of substituted and non-substituted poly(lactide)s, i.e., poly(d-2-hydroxybutyrate) and poly(l-lactide) chains having the opposite configurations [P(D-2HB)-b-PLLA] and the reference block copolymer of poly(d-2-hydroxybutyrate) and poly(d-lactide) chains with the identical configurations [P(D-2HB)-b-PDLA] was investigated. At the crystallizable temperature range of 60-160 °C, the crystallized P(D-2HB)-b-PLLA contained solely the hetero-stereocomplex crystallites as a crystalline species, without formation of poly(d-2-hydroxybutyrate) or poly(l-lactide) homo-crystallites, in contrast with their polymer blends. On the other hand, at the crystallizable temperature range of 60-140 °C, the crystallized P(D-2HB)-b-PDLA had only PDLA homo-crystallites as crystalline species, reflecting no co-crystallites formation between poly(d-2-hydroxybutyrate) and poly(d-lactide) chains having the same configurations. The equilibrium melting temperature of hetero-stereocomplex crystallites in P(D-2HB)-b-PLLA was 189.0 °C, which was higher than 171.3 °C of PDLA homo-crystallites in P(D-2HB)-b-PDLA. Although the final crystallinity of P(D-2HB)-b-PLLA was higher than those of P(D-2HB)-b-PDLA, the spherulite growth rate of P(D-2HB)-b-PLLA was lower.The regime analysis indicated unusual nucleation mechanism of P(D-2HB)-b-PLLA.     

Survey of actual viewing conditions at home and appropriate luminance of LCD‐TV screens

Abstract— To understand actual viewing conditions at home is important for TV design. And the preferred luminance level of LCD TVs under actual viewing conditions is also important in order to obtain both good picture quality and low power consumption. The actual viewing conditions of households and the preferred luminance levels was investigated. In a field test of 83 households, the display luminance, screen illuminance, and viewing locations were measured on site. In laboratory experiments, young and elderly subjects adjusted the luminance of an LCD‐TV screen to their preferred levels under different screen illuminance levels, angular screen sizes, and average luminance levels (ALL) of the images. As a result, two equations, which represent the preferred luminance level of LCD‐TV screens corresponding to different viewing conditions for young and elderly subjects were obtained. When the ALL of the images was 25% and the screen illuminance and angular screen size were set at 100 lx and 20°, respectively, the preferred luminance was 1 60 cd/m² for the young subjects and 248 cd/m² for the elderly subjects. By using the setting of the preferred luminance of an LCD TV under actual viewing conditions, it is possible to conserve energy consumption.     

Platform design for large-scale artificial market simulation and preliminary evaluation on the K computer

Artificial market simulations have the potential to be a strong tool for studying rapid and large market fluctuations and designing financial regulations. High-frequency traders, that exchange multiple assets simultaneously within a millisecond, are said to be a cause of rapid and large market fluctuations. For such a large-scale problem, this paper proposes a software or computing platform for large-scale and high-frequency artificial market simulations (Plham: /pl\(\Lambda\)m). The computing platform, Plham, enables modeling financial markets composed of various brands of assets and a large number of agents trading on a short timescale. The design feature of Plham is the separation of artificial market models (simulation models) from their execution (execution models). This allows users to define their simulation models without parallel computing expertise and to choose one of the execution models they need. This computing platform provides a prototype execution model for parallel simulations, which exploits the variety in trading frequency among traders, that is, the fact that some traders do not require up-to-date information of markets changing in millisecond order. We evaluated a prototype implementation on the K computer using up to 256 computing nodes.     

Extraction and implementation of muscle synergies in neuro-mechanical control of upper limb movement

This work faces the redundancy problem, a central concern in robotics, in a particular force-producing task by using muscle synergies to simplify the control. We extracted muscle synergies from human electromyograph signals and interpreted the physical meaning of the identified muscle synergies. Based on the human analysis results, we hypothesized a novel control framework that can explain the mechanism of the human motor control. The framework was tested in controlling a pneumatic-driven robotic arm to perform a reaching task. This control method, which uses only two synergies as manipulated variables for driving antagonistic pneumatic artificial muscles to generate desired movements, would be useful to deal with the redundancy problem; thus, suggesting a simple but efficient control for human-like robots to work safely and compliantly with humans.     

Comparison of land surface emissivity and radiometric temperature derived from MODIS and ASTER sensors

This study compared surface emissivity and radiometric temperature retrievals derived from data collected with the MODerate resolution Imaging Spectroradiometer (MODIS) and Advanced Spaceborne Thermal Emission Reflection Radiometer (ASTER) sensors, onboard the NASA's Earth Observation System (EOS)-TERRA satellite. Two study sites were selected: a semi-arid area located in northern Chihuahuan desert, USA, and a Savannah landscape located in central Africa. Atmospheric corrections were performed using the MODTRAN 4 atmospheric radiative transfer code along with atmospheric profiles generated by the National Center for Environmental Predictions (NCEP). Atmospheric radiative properties were derived from MODTRAN 4 calculations according to the sensor swaths, which yielded different strategies from one sensor to the other. The MODIS estimates were then computed using a designed Temperature-Independent Spectral Indices of Emissivity (TISIE) method. The ASTER estimates were derived using the Temperature Emissivity Separation (TES) algorithm. The MODIS and ASTER radiometric temperature retrievals were in good agreement when the atmospheric corrections were similar, with differences lower than 0.9 K. The emissivity estimates were compared for MODIS/ASTER matching bands at 8.5 and 11 μm. It was shown that the retrievals agreed well, with RMSD ranging from 0.005 to 0.015, and biases ranging from −0.01 to 0.005. At 8.5 μm, the ranges of emissivities from both sensors were very similar. At 11 μm, however, the ranges of MODIS values were broader than those of the ASTER estimates. The larger MODIS values were ascribed to the gray body problem of the TES algorithm, whereas the lower MODIS values were not consistent with field references. Finally, we assessed the combined effects of spatial variability and sensor resolution. It was shown that for the study areas we considered, these effects were not critical.     

Growth monitoring and classification of rice fields using multitemporal RADARSAT-2 full-polarimetric data

Spaceborne synthetic aperture radar (SAR) can be used for agricultural monitoring. In this study, three single-polarimetric and four full-polarimetric observation data sets were analysed. A rice paddy field in northern Japan was used as the study site; the data for this site were obtained using RADARSAT-2, which carries a full-polarimetric C-band SAR. Soybean and grass fields were also present within the paddy fields. The temporal change in the backscattering coefficient of the rice paddy fields for the single-polarization data agreed with the temporal change obtained for a rice growth model based on radiative transfer theory. A three-component decomposition approach was applied to the full-polarimetric data. With each rice growth stage, the volume scattering component ratio increased, whereas the surface scattering component ratio generally decreased. The soybean and grass fields showed a smaller double-bounce scattering component than the rice fields for all the acquired data. The results of this study show that multitemporal observation by full-polarimetric SAR has great potential to be utilized for estimating rice-planted areas and monitoring rice growth.     

Effects of the aluminum concentration on twin boundary motion in pre-strained magnesium alloys

We investigated the effects of Al concentration on the reciprocated motion of twin boundaries in pre-strained Mg-Al-Zn alloys through a combination of applied compression and tension,in-situ electron-backscattering diffraction observations,and high-angle annular dark-field scanning transmission electron microscopy observations.The twin growth was restricted by increased Al concentration,which resulted in the occurrence of smaller-sized twins.The reverse motion of twin boundaries was also restricted,resulting in the formation of higher fractions of secondary twins and 2-5° boundaries during reverse tension.The secondary twins and 2-5° boundaries mainly contributed to the increased ultimate tensile strength of the pre-strained Mg alloys.This effect is more significant in Mg alloys with larger pre-compression.Moreover,the increased amount of the Al solute atoms,rather than the precipitates,mainly contributed to the increased strengthening effect on the twin boundary motion.Our research contributes to development of high-strength Mg alloys by stabilizing twin boundaries.