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排序方式: 共有218条查询结果,搜索用时 15 毫秒
1.
Cagnon Christine; Valverde Viviane; Masson Jean-Michel 《Protein engineering, design & selection : PEDS》1991,4(7):843-847
A set of 11 expression vectors was constructed, each of themharbouring a cloning cassette under the control of the promoter.Some of these vectors enable expression of foreign proteinsin the cytoplasm, while others include a synthetic sequencecoding for a very efficient secretion signal sequence. Otherfeatures are an fl origin of replication (in plus or minus orientation)and a promoterup mutation that enhances the already very highlevel of expression from these vectors. With such a versatilevector family, cloning, sequencing and sitedirected mutagenesiscan be performed on the same vector, and the level of expressioncan be defined according to the specific constraints of a givenprotein. 相似文献
2.
Reda Karoum Antonio de Lucas-Consuegra Fernando Dorado Jose Luis Valverde Alain Billard Philippe Vernoux 《Journal of Applied Electrochemistry》2008,38(8):1083-1088
The electrochemical promotion of catalysis (EPOC) of propene combustion was investigated using Pt sputtered thin film on an
O2− conductor, 8 mol% Y2O3-stabilized-ZrO2 (YSZ). In order to separate the influence of the thermal migration of the O2− oxide ions from the electrolyte to the catalyst surface and the impact of an electrical polarization on the catalytic activity,
several light-off experiments (cool down and heat up procedures) were successively performed under different polarizations,
i.e. OCV, +2 and −2 V. These experiments have clearly shown that the presence of O2− (thermally or electrochemically induced) inhibits the catalytic activity of the platinum for the propene deep oxidation.
These results demonstrate the importance to define a normalized rate enhancement ratio, ρ
n
, from a reference value of the catalytic rate corresponding to a Pt surface state free of O2− ions. 相似文献
3.
N. Valverde 《Journal of Applied Electrochemistry》1981,11(3):305-312
Symmetric solid-electrolyte galvanic cells such as Pt/Ag2± Se/RbAg4+yI5–ySey/Ag2± Se/Pt, based on a continuous variation of the redox potential of the storage phase Ag2± Se with its composition, show considerable advantages over other types of cells, e.g. high accuracy, long charge-storage capability, effectively a double range of potential variation, and suppression of anodic polarization at the metalelectrolyte interface. The temperature characteristics as well as the overvoltage of this cell have been quantitatively determined and analysed. This cell can discharge in the temperature range –30 to 130° C with a current density up to 10 mA cm–2. 相似文献
4.
5.
Cork quality classification system using a unified image processingand fuzzy-neural network methodology 总被引:3,自引:0,他引:3
Joongho Chang Gunhee Han Valverde J.M. Griswold N.C. Duque-Carrillo J.-F. Sanchez-Sinencio E. 《Neural Networks, IEEE Transactions on》1997,8(4):964-974
Cork is a natural material produced in the Mediterranean countries. Cork stoppers are used to seal wine bottles, Cork stopper quality classification is a practical pattern classification problem. The cork stoppers are grouped into eight classes according to the degree of defects on the cork surface. These defects appear in the form of random-shaped holes, cracks, and others. As a result, the classification cork stopper is not a simple object recognition problem. This is because the pattern features are not specifically defined to a particular shape or size. Thus, a complex classification form is involved, Furthermore, there is a need to build a standard quality control system in order to reduce the classification problems in the cork stopper industry. The solution requires factory automation meeting low time and reduced cost requirements. This paper describes a cork stopper quality classification system using morphological filtering and contour extraction and following (CEF) as the feature extraction method, and a fuzzy-neural network as a classifier. This approach will be used on a daily basis. A new adaptive image thresholding method using iterative and localized scheme is also proposed, A fully functioning prototype of the system has been built and successfully tested. The test results showed a 6.7% rejection ratio, It is compared with the 40% counterpart provided by traditional systems. The human experts in the cork stopper industry rated this proposed classification approach as excellent. 相似文献
6.
Ana B. Bautista‐Ortín Naiara Busse‐Valverde Jose M. López‐Roca Rocío Gil‐Muñoz Encarna Gómez‐Plaza 《International Journal of Food Science & Technology》2014,49(1):34-41
Winemakers are interested in wines with low bitterness and astringency, and therefore, there is a preference for a higher presence of skin proanthocyanidins in wines. However, the information regarding the effect of reducing or eliminating seed proanthocyanidins from the must or fermenting wine on the characteristics of finished wines is scarce. For this reason, we elaborated a wine using Monastrell grapes from which the seeds had been removed and compared it with a control wine (CW) made from intact grapes. The anthocyanin and proanthocyanidin concentration and profile, and the chromatic and sensory characteristics were studied. The absence of seed proanthocyanidins did not affect anthocyanin concentration, although the proanthocyanidin concentration was 40% lower than in the CW. The copigmentation (CA) study showed that both wines had a similar extent of CA phenomena although colour intensity was higher when seed proanthocyanidins were present. The panellist evaluated the wines elaborated without seeds as being fruitier, less astringent and with an overall higher quality. 相似文献
7.
8.
Dr. Pablo Valverde J. Daniel Martínez Prof. F. Javier Cañada Dr. Ana Ardá Prof. Jesús Jiménez-Barbero 《Chembiochem : a European journal of chemical biology》2020,21(21):2999-3025
Carbohydrates play a pivotal role in intercellular communication processes. In particular, glycan antigens are key for sustaining homeostasis, helping leukocytes to distinguish damaged tissues and invading pathogens from healthy tissues. From a structural perspective, this cross-talk is fairly complex, and multiple membrane proteins guide these recognition processes, including lectins and Toll-like receptors. Since the beginning of this century, lectins have become potential targets for therapeutics for controlling and/or avoiding the progression of pathologies derived from an incorrect immune outcome, including infectious processes, cancer, or autoimmune diseases. Therefore, a detailed knowledge of these receptors is mandatory for the development of specific treatments. In this review, we summarize the current knowledge about four key C-type lectins whose importance has been steadily growing in recent years, focusing in particular on how glycan recognition takes place at the molecular level, but also looking at recent progresses in the quest for therapeutics. 相似文献
9.
10.
Tools to maintain postharvest fruit and vegetable quality through the inhibition of ethylene action: a review 总被引:2,自引:0,他引:2
Martínez-Romero D Bailén G Serrano M Guillén F Valverde JM Zapata P Castillo S Valero D 《Critical reviews in food science and nutrition》2007,47(6):543-560
Ethylene is a plant hormone controlling a wide range of physiological processes in plants. During postharvest storage of fruit and vegetables ethylene can induce negative effects including senescence, over-ripening, accelerated quality loss, increased fruit pathogen susceptibility, and physiological disorders, among others. Apart from the endogenous ethylene production by plant tissues, external sources of ethylene (e.g. engine exhausts, pollutants, plant, and fungi metabolism) occur along the food chain, in packages, storage chambers, during transportation, and in domestic refrigerators. Thus, it is a great goal in postharvest to avoid ethylene action. This review focuses on tools which may be used to inhibit ethylene biosynthesis/action or to remove ethylene surrounding commodities in order to avoid its detrimental effects on fruit and vegetable quality. As inhibitors of ethylene biosynthesis and action, good results have been found with polyamines and 1-methylcyclopropene (1-MCP) in terms of maintenance of fruit and vegetable quality and extension of postharvest shelf-life. As ethylene scavengers, the best results can be achieved by adsorbers combined with catalysts, either chemical or biological (biofilters). 相似文献