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Mri Miczi Mria Golda Balzs Kunkli Tibor Nagy Jzsef Tzsr Jnos Andrs Mtyn 《International journal of molecular sciences》2020,21(24)
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His6-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2. 相似文献
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1 INTRODUCTIONParticlesreinforcedtitaniumalloymatrixcomposites(TMCp)havewideapplicationfieldsfortheirattractivehighspecificstreng 相似文献
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Kahler Christopher W.; Read Jennifer P.; Wood Mark D.; Palfai Tibor P. 《Canadian Metallurgical Quarterly》2003,17(3):226
Structural equation modeling was used to test the mediational role of social environmental selection on alcohol use in cross-sectional samples of 447 students from a rural state university and 421 students from an urban private university. Results showed that male gender, White ethnicity, and sensation seeking were uniquely associated with greater alcohol use. Mediational analyses indicated that socioenvironmental factors (i.e., Greek involvement, friends' approval of drinking/getting drunk) were positively associated with alcohol use and significantly accounted for parts of the effects of ethnicity and sensation seeking, but not gender, on alcohol use. Results suggest that White students and those high on sensation seeking may drink more heavily in college, in part because they select social environments in which alcohol use is encouraged. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Er. V. Kal’yanov 《Technical Physics Letters》2007,33(12):1015-1017
A mathematical model of a new controllable autooscilatory chaotic system based on inductively coupled Chua’s oscillators is described. Numerical simulations show that, using a chaotizing feedback algorithm, chaotic oscillations in this system can be excited in regimes where only regular oscillations are generated otherwise. 相似文献
6.
国内外钻探用金刚石复合片性能的研究 总被引:1,自引:0,他引:1
主要介绍国内外金刚石复合片性能及采用四高复合片钻探硬岩的初试,为快速钻探提供一个新的途径. 相似文献
7.
A method for multi-parameter PDF estimation of random variables 总被引:1,自引:1,他引:0
Guo-Kang Er 《Structural Safety》1998,20(1):25-36
The probability distribution function (PDF) of a random variable Z is approximated with c·eQ(z), where Q(z) is a polynomial function and c is normalizing constant. Based upon the weighted residual method, general linear algebraic equations have been derived for the evaluation of the unknown parameters in the polynomial. Numerical examples are presented and the results show that the PDFs obtained using the proposed method converge to those obtained from Monte Carlo simulation as the number of parameters in the approximate PDF increases. 相似文献
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We have proposed that application of singular value decomposition on finite-difference time-domain simulations is best suited for modal analysis of complex real-life electromagnetic problems. This combination of two powerful techniques yields a wealth of information about the complete mode structure which cannot be otherwise obtained. This is particularly important for a real-life system having complex geometry and consisting of a range of materials from good conductors to dielectrics and this is further complicated by the existence of broad-band excitation. This hybrid approach is versatile, accurate, and computationally efficient. 相似文献
10.
The present investigation showed that the re-laxation peak at about 200℃ (f≈1Hz) was relatedto bainitic transformation in a CuZnAl alloy.Thepeak decreased when bainite precipitated.Theactivation energy of the peak was the same as theapparent activation energy of bainitic transforma-tion.According to the relations between the peakand the bainitic transformation,the mechanism ofthe peak has been discussed. 相似文献