Immunoaffinity-chromatography purification of salbutamol in liver and HPLC-fluorometric detection at trace residue level

Summary A method combining immunoaffmity-chromatography (IAC) and high-performance liquid chromatography (HPLC) for the analysis of Salbutamol in liver with a low quantification limit of 1 g/kg has been developed. Salbutamol was extracted with 0.01 mol/L HCl and purified by IAC. The samples were analysed on a liquid Chromatograph fitted with a C₁₈ -Bondapak column. A fluorometer was used for the detection of salbutamol. Recoveries of 67–80% could be obtained.

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Immunoaffinitätschromatographische Reinigung von Salbutamol in Leber und Rückstandsbestimmung mittels HPLC und fluorimetrischer Detektion

Zusammenfassung Eine Methode, die Immunoaffinitäts-und Hochleistungsflüssigchromatographie verbindet, ist für die Analyse von Salbutamol in Leber entwickelt worden. Eine niedrige Bestimmungsgrenze von 1 g/kg Leber wurde erreicht. Salbutamol wurde mit 0,01 mol/L Salzsäure freigesetzt und durch Immunoaffinitätschromatographie gereinigt. Die Proben wurden durch Flüssigchromatographie auf einer C₁₈ -Bondapak-Säule analysiert. Ein Fluorimeter wurde für die Detektion von Salbutamol benutzt. Ausbeuten von 67 bis 80% konnten erreicht werden.

     

ASSIMILATION AND ATTACHMENT IN THE CONTEXT OF INTERNATIONAL MIGRATION: THE CASE OF GUESTWORKERS IN GERMANY

"Immigrants' attachments to home and their assimilation into the host society have been identified as major factors influencing international return migration. This paper provides an empirical analysis of the determinants of attachment and assimilation, with a special focus on the impact of duration of stay. Using survey data on guestworkers in West Germany in 1984 and 1989, the study finds that assimilation increases at a decreasing rate as the duration of stay is extended. The relationship between attachment and length of stay is less strong, but shows a general trend of decreasing attachment levels as guestworkers prolong their stay in Germany. The resulting trends for return migration are characterized by an overall decline in the propensity to return as the duration of stay is extended. However, the rate of decline varies by gender, marital status, and nationality. Overall, the results allude to the importance of distinguishing between short term and long term immigrants."     

Editors’ note

Editorial Note

Editors’ note     

Robust FIPP p-cycles against dual link failures

We propose a new generic flow formulation for Failure-Independent Path-Protecting (FIPP) p-cycles subject to multiple failures. While our new model resembles the decomposition model formulation proposed by Orlowski and Pioro (Networks, 2011) in the case of classical shared path protection, its originality lies in its adaptation to FIPP p-cycles. When adapted to that last pre-configured pre-cross connected protection scheme, the bandwidth sharing constraints must be handled in a different way in order to take care of the sharing along the FIPP p-cycles. It follows that, instead of a polynomial-time solvable pricing problem as in the model of Orlowski and Pioro (Networks, 2011), we end up with a much more complex pricing problem, which has an exponential number of constraints due to some subtour elimination constraints. Consequently, in order to efficiently solve the pricing problem, we consider: (i) a hierarchical decomposition of the original pricing problem; (ii) heuristics in order to go around the large number of constraints in the pricing problem. Performance evaluation is made in the case of FIPP p-cycles subject to dual failures. For small to medium size networks, the proposed model remains fairly scalable for increasing percentages of dual failures, and requires much less bandwidth than p-cycle protection schemes (ratio varies from 2 to 4). For larger networks, heuristics are required in order to keep computing times reasonable. In the particular case of single link failures, it compares very favorably (5 to 10 % of bandwidth saving) to the previously proposed column generation ILP model of Rocha, Jaumard and Stidsen (Telecommun. Syst., 2012).     

Design and dimensioning of hybrid PONs

We investigate the greenfield deployment of hybrid time division multiplexing/wavelength division multiplexing passive optical networks, i.e., the optimized covering of a given geographical area by a set of cost-effective and properly dimensioned hybrid PONs. We first propose a three-phase optimization scheme. In the first phase, a p-center model is proposed to determine the best ONU clusterings and the lower-level passive distribution node equipment locations. The second phase takes care of the placement of the upper-level passive distribution node equipment and the third one, using the traffic demands, a mix of unicast and multicast requests, selects the most appropriate passive distribution node equipment, i.e., either an arrayed waveguide gratings or a splitter. We next merge the first two phases, leading then to a two-phase scheme. Computational experiments are conducted on two sets of ONUs, one with 128 and one with 512 ONUs. We compare the two- and three-phase schemes in terms of PON design costs. We observe that the two-phase scheme provides more cost-effective solutions. We also provide a sensitivity analysis with respect to several parameters in order to identify the number of ONU clusters that minimizes the deployment cost.     

Capsosomes with Multilayered Subcompartments: Assembly and Loading with Hydrophobic Cargo

Therapeutic artificial cells or organelles are nanoengineered vehicles that are expected to substitute for missing or lost cellular function. The creation of capsosomes, polymer carrier capsules containing liposomal subcompartments, is a promising approach towards constructing such therapeutic devices using the layer‐by‐layer assembly method. Herein, the assembly of intact, nonaggregated capsosomes containing multiple liposome layers is reported. It is also further demonstrated that thiocoraline, a hydrophobic model peptide with antitumor activity, can be efficiently loaded into the membrane of the liposomal subcompartments of the capsosomes. Cell viability assays verify the activity of the trapped antitumor cargo. It is also shown that pristine capsosomes do not display inherent cytotoxic effects. The ability to tune the number of liposome layers and hence the drug loading in capsosomes as well as their noncytotoxicity provide new opportunities for the creation of therapeutic artificial cells and organelles.     

87Sr/86Sr from rock and soil into vine and wine

For provenance assignments of wines strontium isotope ratios can be used because soils from different wine-growing regions, and hence the wines grown there, each show specific ratios. Some successful applications are demonstrated.

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⁸⁷Sr/⁸⁶Sr aus Gestein und Boden in Rebe und Wein

Zusammenfassung Zur Herkunftsbestimmung von Weinen können Strontium-Isotopenverhältnisse herangezogen werden, da die Böden verschiedener Weinbaugebiete, und damit die dort gezogenen Weine, jeweils spezifische Verhältnisse aufweisen. Einige erfolgreiche Anwendungen werden demonstriert.

     

A novel Topology Aggregation approach for shared protection in multi-domain networks

Routing for shared protection in multi-domain networks is more difficult than that in single-domain networks because of the scalability requirements. We propose a novel approach for shared protection routing in multi-domain networks where the key feature is a special Topology Aggregation. In this Topology Aggregation, only some potential intra-domain paths (intra-paths for short) are selected for carrying working and backup traffic between domain border nodes. The abstraction of each intra-path to a virtual edge makes the original multi-domain network to become an aggregated network. On the aggregated network, a single-domain routing algorithm for shared protection can be applied for obtaining the complete routing solutions. The experiments show that the proposed approach is scalable. Moreover it is close to the optimal solution in single-domain networks and outperforms the previously proposed scalable solutions in multi-domain networks.