Gaschromatographische Bestimmung der h?heren Ester in Weindestillat, Weinbrand, Branntwein und Wein. II

Zusammenfassung Auf Grund der Beobachtung, daß freie Fettsäuren die Bestimmung höherer Äthylester \ bei gaschromatographischer Trennung an Carbowax 20 M stören können, wurden Reihenversuche zur Eliminierung dieser Störpartner durchgeführt. Dabei erwies sich Natriumhydrogencarbonat günstiger als Natriumcarbonat. Eine geeignete Methode zur Bestimmung der gradkettigen Äthylester der Capron- bis Stearinsäure, die auch für die Äthylester von ungesättigten C₁₆- und C₁₈-Säuren verwendet werden kann, wird angegeben.

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Determination of the ethyl esters of long chain fatty acids in wine distillate, brandy, and wine. II

Summary Free fatty acids are able to interfere the determination of the ethyl esters by gas chromatography on Carbowax 20 M. Therefore series of tests were made to remove the fatty acids. Sodium hydrogen carbonate was found to be more suitable than sodium carbonate. A method is described to determine the ethyl esters of fatty acids of caproic to stearic acid and of the unsaturated fatty acids with 16 and 18 carbon atoms.

     

Doping efficiency and energy-level scheme in C60F48-doped zinc–tetraphenylporphyrin films

High resolution synchrotron-based core level spectroscopy was used to examine the energy level alignment at the interface of zinc–tetraphenylporphyrin films doped by the surface acceptor C₆₀F₄₈. Two distinct fluorofullerene charge states were identified, corresponding to ionized and neutral molecules, and their relative concentration as a function of coverage was used to evaluate the probability of occupation of the acceptor lowest unoccupied molecular orbital (LUMO). From an initial acceptor energy of ?0.25 eV, the C₆₀F₄₈ LUMO shifts upwards with coverage due to a doping-induced interfacial dipole potential, and stabilization of the LUMO at an energy 0.45 eV above the Fermi energy was obtained. While the energy difference upon saturation is consistent with the results obtained for other donor–acceptor systems that have been interpreted as Fermi level pinning, the present work shows that the energy offset is a direct consequence of the interplay between Fermi–Dirac statistics in combination with the interfacial dipole potential.     

Nanoporous gold bumps for low temperature bonding

Nanoporous gold bumps have been deposited on silicon wafers by electroplating a silver–gold alloy followed by etching the silver. An open-porous cellular structure of gold at meso-scale is left on top of the bumps. For flip chip bonding we found low temperature and low force bonding conditions. The porous interconnects have very promising properties, like compressibility and reduced stiffness, which should result in higher bond yield and extended reliability.     

Ultrathin Polydopamine Films with Phospholipid Nanodiscs Containing a Glycophorin A Domain

Cellular membranes have long served as an inspiration for nanomaterial research. The preparation of ultrathin polydopamine (PDA) films with integrated protein pores containing phospholipids and an embedded domain of a membrane protein glycophorin A as simplified cell membrane mimics is reported. Large area, ultrathin PDA films are obtained by electropolymerization on gold surfaces with 10–18 nm thickness and dimensions of up to 2.5 cm². The films are transferred from gold to various other substrates such as nylon mesh, silicon, or substrates containing holes in the micrometer range, and they remain intact even after transfer. The novel transfer technique gives access to freestanding PDA films that remain stable even at the air interfaces with elastic moduli of ≈6–12 GPa, which are higher than any other PDA films reported before. As the PDA film thickness is within the range of cellular membranes, monodisperse protein nanopores, so‐called “nanodiscs,” are integrated as functional entities. These nanodisc‐containing PDA films can serve as semi‐permeable films, in which the embedded pores control material transport. In the future, these simplified cell membrane mimics may offer structural investigations of the embedded membrane proteins to receive an improved understanding of protein‐mediated transport processes in cellular membranes.     

A preamble-based approach for providing quality of service support in wireless sensor networks

Medium Access Control (MAC) protocols for Wireless Sensor Networks (WSN) are usually designed as random access protocols that apply different kinds of backoff strategies since Time Division Multiple Access (TDMA) based protocols with admission control are very complex and require additional mechanisms for synchronization. Without such mechanisms, fair or priority based medium access with Quality of Service (QoS) guarantees can hardly be achieved by existing protocols. Therefore, we developed a random access protocol which uses a new preamble-based medium access strategy that enables collision-free priority based access without the need of synchronization. In this paper we introduce different QoS strategies and their use cases. All strategies can be easily integrated in our protocol to meet the requirements of different target applications. Furthermore, we compare the performance of the strategies with a typical carrier-sense based protocol.     

High-resolution venography of the brain using magnetic resonance imaging

The purpose of this study was to evaluate a non-flow related magnetic resonance imaging method to visualize small veins independent of arteries in the human brain. A long TE, high-resolution 3D gradient echo MR acquisition was used to highlight venous information. The method is based on the paramagnetic property of deoxyhemoglobin and the resulting phase difference between veins and brain parenchyma at long echo times. The MR magnitude images were masked with a phase mask filter to enhance small structure visibility.. Venous information down to sub-pixel vessel diameters of several hundred microns is visible. Venous data are displayed in an angiographic manner using a minimum intensity projection algorithm. Both superficial veins and deep white matter veins are visible. The method has been successfully applied in volunteers. Preliminary results in patients with cerebral arteriovenous malformations indicate its potential in clinical applications. The proposed method is easy to implement and does not require administration of a contrast agent or application of specially designed rf pulses to highlight the veins. Rather it exploits the intrinsic magnetic properties (BOLD-effect) and the prolonged T ^_2* of venous blood. The method may be of diagnostic potential in the assessment of arteriovenous malformations or other vascular venous lesions. © 1998 Elsevier Science B.V. All rights reserved.     

Pro-oxidative effects of melanoidin–copper complexes on isolated and cellular DNA

High molecular weight Maillard reaction products (melanoidins) are described to possess metal-chelating properties. Whereas in food systems, this ability contributes to antioxidant properties, the consequences on biological systems are not quite clear. The study was aimed to evaluate the implication of metal complexation by melanoidins on DNA damage. Melanoidins prepared with d-glucose and different l-amino acids under water-free reaction conditions were charged with cupric ions. The effect on isolated DNA was investigated by the PM2 assay and on cellular systems in the human colon carcinoma cell line HCT-116 by alkaline unwinding. Independent of the amino acid composition, pure melanoidins (MW >14 kDa) did not cause significant DNA damage. By charging melanoidins with Cu²⁺ ions, a considerable DNA strand breaking activity was detectable, which was again amplified in an oxidative milieu (addition of hydrogen peroxide). Since Cu²⁺ normally does not provoke the formation of reactive oxygen species (ROS) via Fenton-type reaction, the results obtained have to be attributed to reducing properties of melanoidins. Thus, in melanoidin–copper complexes redox cycling may take place leading to Cu⁺ which subsequently undergoes Fenton-type and Haber–Weiss reactions. As a consequence, ROS are formed, which may explain the generation of DNA strand breaks.