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Sara da Mata Joana Ferreira Inmaculada Nicols Susana Esteves Gonalo Esteves Sofia Lrias Fernanda Silva Adela Saco Daniela Cochicho Mrio Cunha Marta del Pino Jaume Ordi Ana Flix 《International journal of molecular sciences》2021,22(5)
The expression of p16 is a good surrogate of human papillomavirus (HPV) infection in HPV-associated cancers. The significance of p16 expression, HPV genotype and genera in the outcome of patients with HPV-associated cervical cancer (CC) is unclear. Our aim is to ascertain the prognostic significance of these factors. Data from 348 patients (median age: 47.5 years old) with CC, diagnosed in two referral centers, were retrospectively collected. Advanced disease (FIGO2018 IB2-IV) was present in 68% of patients. A single HPV genotype was identified in 82.8% of patients. The most common HPVs were HPV16 (69%) and HPV18 (14%). HPV genera reflected this distribution. HPV16 tumors presented at an earlier stage. P16 was negative in 18 cases (5.2%), 83.3% of which were squamous cell carcinomas. These cases occurred in older patients who tended to have advanced disease. In the univariate analysis, HPV16 (HR: 0.58; p = 0.0198), α-9 genera (HR: 0.37; p = 0.0106) and p16 overexpression (HR: 0.54; p = 0.032) were associated with better survival. HPV16 (HR: 0.63; p = 0.0174) and α-9 genera (HR: 0.57; p = 0.0286) were associated with less relapse. In the multivariate analysis, only the International Federation of Gynecology and Obstetrics (FIGO) stage retained an independent prognostic value. HPV16, α-9 genera and p16 overexpression were associated with better survival, although not as independent prognostic factors. Patients with p16-negative HPV-associated CC were older, presented with advanced disease and had worse prognosis. 相似文献
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A. Redondo M. José Villanueva M. Dolores Rodriguez M. Dolores Saco 《European Food Research and Technology》1997,205(6):457-463
Different methods have been used to evaluate variations in the dietary fibre content of carrots and turnips during autoclaving. Autoclaving was carried out at 121°C under pressure for 15 min. The methods used were the gravimetric (neutral and acid detergent fibre) method, the enzymatic-gravimetric method for (insoluble and soluble fibre and the spectrophotometric method for pectic substances. When the methodologies were compared it was observed that in raw carrots and turnips, the neutral detergent fibre and insoluble fibre contents were statistically different (P<0.05). This was not the case for processed samples of the vegetables. The correlation between the different methodologies was studied. A good correlation was found between neutral detergent fibre and insoluble fibre in raw turnips (r = 0.999). Variance analysis indicated that quantitative variations resulting after thermal treatment were statistically significant (P<0.05) for neutral detergent fibre in carrots (raw = 1.97%, processed = 1.68%), and for pectic substances in carrots (raw = 0.54%, processed = 0.47%) and turnips (raw = 0.33%, processed = 0.25%). Optical microscopy has been used to identify different dietary fibre components in raw and processed samples. 相似文献
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María-José Villanueva María-Dolores Rodríguez Araceli Redondo María-Dolores Saco 《European Food Research and Technology》1996,203(4):345-350
Modifications of dietary fibre in beetroot during autoclaving have been evaluated by different methods: acid detergent (raw=1.45%; processed=1.17%) and neutral detergent (raw=2.30%; processed=2.00%), the enzymatic-gravimetric method of Asp (raw=3.35%; processed=3.34%), HPLC (raw=1.42%; processed=1.60%) and 3,5-dimethylphenol (raw=0.36; processed=0.28%). The correlation between different methodologies was studied. Autoclaving was carried out at 121°C under pressure for 15 min. Variance analysis indicated that quantitative variations originating in the thermic treatment were not statistically significant (P>0.05). 相似文献
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The precipitation temperatures of sodium caseinate in H2O and D2O in the presence of Mg2+, Ca2+, Sr2+ and Ba2+ were investigated through fluorescence, turbidity and conductivity experiments. 相似文献
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Hofmeister phenomena: an update on ion specificity in biology 总被引:1,自引:0,他引:1
6.
Three-dimensional numerical investigation of PEMFC with landing to channel ratio (L:C) of 2:2 for 25-cm2 serpentine-parallel channel has been simulated, and the obtained results have been validated with the polarization curve obtained through experiments. It is found that the maximum error in the polarization curve is less than 4%, and thus a very good deal exists between the simulation study and experimentation. Upon validation, the study has been extended for various flow path designs with different L:C ratio numerically. The prediction reveals that the L:C ratio of 2:2 exhibits the better performance for all the flow channels considered, and it is found that the straight-zigzag flow field with L:C ratio of 2:2 attributes the maximum power density of 0.3250 W/cm2 for an optimum open circuit voltage of 0.4 Volts with minimal pressure drop. Oxygen consumption in the cathode flow channels of serpentine-parallel, serpentine-zigzag, and straight-parallel are 77.08%, 10.41%, and 42.70% lesser than that of straight-zigzag PEMFC, respectively. The pressure drop in the flow channel of serpentine-parallel, serpentine-zigzag, and straight-parallel with landing to channel ratio 2:2 are 78.18%, 95.81%, and 48.33% higher than that of straight-zigzag flow field, respectively. The polarization curve, hydrogen (H2), oxygen (O2), water content along the flow channel and the proton conductivity, H2O content across the membrane electrolyte, and current density contour at the GDL/catalyst interface of the anode side for all flow channel configurations have been presented and discussed. 相似文献
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The present study focuses on three‐dimensional two‐phase CFD investigation on scaled‐up proton exchange membrane fuel cell (PEM‐FC) for an active area of 100 cm2 with different designs of serpentine and parallel flow configuration. The humidification of hydrogen and oxygen is varied from 10% to 100% to study the PEM‐FC performance. The numerical results of polarization curves predicted in this study have been numerically validated with that of the literature for both parallel and counter serpentine flow channels with active area of 24.8‐cm2 PEM‐FC. Further upon validation, the numerical study is extended for scaled‐up PEM‐FC with active area of 100 cm2 with different flow path designs to study its performance characteristics namely polarization curves, species concentration distribution, water content in the membrane electrolyte, and proton conductivity to evaluate the fuel cell performance. The three‐dimensional CAD models are created in SOLIDWORKS 10.0 and are discretised hexahedrally using finite volume method. The various governing equations namely conservation of mass, momentum, energy, species concentration, and electrochemical equations are solved numerically with the necessary boundary conditions using the CFD code. The novel design of straight zigzag flow path shows the better performance output over the other designs investigated which is having a higher power density of 0.3711 W/cm2 for 100% relative humidity of reactant and oxidant. 相似文献
10.
María-José Villanueva María-Dolores Rodríguez Araceli Redondo María-Dolores Saco 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1996,203(4):345-350
Modifications of dietary fibre in beetroot during autoclaving have been evaluated by different methods: acid detergent (raw=1.45%; processed=1.17%) and neutral detergent (raw=2.30%; processed=2.00%), the enzymatic-gravimetric method of Asp (raw=3.35%; processed=3.34%), HPLC (raw=1.42%; processed=1.60%) and 3,5-dimethylphenol (raw=0.36; processed=0.28%). The correlation between different methodologies was studied. Autoclaving was carried out at 121°C under pressure for 15 min. Variance analysis indicated that quantitative variations originating in the thermic treatment were not statistically significant (P>0.05). 相似文献