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排序方式: 共有88条查询结果,搜索用时 15 毫秒
1.
Johann Kalchman Shingo Fujioka Seok Chung Yamato Kikkawa Toshihiro Mitaka Roger D. Kamm Kazuo Tanishita Ryo Sudo 《Microfluidics and nanofluidics》2013,14(6):969-981
Most anti-cancer drug screening assays are currently performed in two dimensions, on flat, rigid surfaces. However, there are increasing indications that three-dimensional (3D) platforms provide a more realistic setting to investigate accurate morphology, growth, and sensitivity of tumor cells to chemical factors. Moreover, interstitial flow plays a pivotal role in tumor growth. Here, we present a microfluidic 3D platform to investigate behaviors of tumor cells in flow conditions with anti-migratory compounds. Our results show that interstitial flow and its direction have significant impact on migration and growth of hepatocellular carcinoma cell lines such as HepG2 and HLE. In particular, HepG2/HLE cells tend to migrate against interstitial flow, and their growth increases in interstitial flow conditions regardless of the flow direction. Furthermore, this migratory activity of HepG2 cells is enhanced when they are co-cultured with human umbilical vein endothelial cells. We also found that migration activity of HepG2 cells attenuates under hypoxic conditions. In addition, the effect of Artemisinin, an anti-migratory compound, on HepG2 cells was quantitatively analyzed. The microfluidic 3D platform described here is useful to investigate more accurately the effect of anti-migratory drugs on tumor cells and the critical influence of interstitial flow than 2D culture models. 相似文献
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Inside Cover: Selective Manipulation of Discrete Mannosidase Activities in the Endoplasmic Reticulum by Using Reciprocally Selective Inhibitors (ChemBioChem 11/2017) 下载免费PDF全文
4.
Dr. Yoshiaki Yasutake Dr. Taiki Nishioka Dr. Noriko Imoto Prof. Tomohiro Tamura 《Chembiochem : a European journal of chemical biology》2013,14(17):2284-2291
Vitamin D3 hydroxylase (Vdh) from Pseudonocardia autotrophica is a cytochrome P450 monooxygenase that catalyzes the two‐step hydroxylation of vitamin D3 (VD3) to produce 25‐hydroxyvitamin D3 (25(OH)VD3) and 1α,25‐dihydroxyvitamin D3 (1α,25(OH)2VD3). These hydroxylated forms of VD3 are useful as pharmaceuticals for the treatment of conditions associated with VD3 deficiency and VD3 metabolic disorder. Herein, we describe the creation of a highly active T107A mutant of Vdh by engineering the putative ferredoxin‐binding site. Crystallographic and kinetic analyses indicate that the T107A mutation results in conformational change from an open to a closed state, thereby increasing the binding affinity with ferredoxin. We also report the efficient biocatalytic synthesis of 25(OH)VD3, a promising intermediate for the synthesis of various hydroxylated VD3 derivatives, by using nisin‐treated Rhodococcus erythropolis cells containing VdhT107A. The gene‐expression cassette encoding Bacillus megaterium glucose dehydrogenase‐IV was inserted into the R. erythropolis chromosome and expressed to avoid exhaustion of NADH in a cytoplasm during bioconversion. As a result, approximately 573 μg mL?1 25(OH)VD3 was successfully produced by a 2 h bioconversion. 相似文献
5.
Kitamura Y Uzawa T Oka K Komai Y Ogawa H Takizawa N Kobayashi H Tanishita K 《Analytical chemistry》2000,72(13):2957-2962
Nitric oxide (NO) is a gaseous mediator involved in various physiological phenomena, such as vasorelaxation and neurotransmission. Investigation of local cellular responses of NO production in vivo and in vitro requires a measurement method with a high spatial resolution. For selective NO measurement, we therefore developed a microcoaxial electrode whose tip diameter is less than 10 microm. Calibration using various concentrations of NO (0.1-1.0 microM) showed that the electrode has good linearity (r = 0.99) and its detection limit is 0.075 microM (S/N = 3). We verified the applicability of this electrode to in vivo and in vitro local measurement NO released from bovine aortic cultured endothelial cells (BAECs) stimulated by acetylcholine (ACh). After the addition of ACh, a transient increase in NO concentration was detected by the electrode. In the presence of NG-nitro-L-arginine methyl ester (L-NAME), a putative NO synthase inhibitor, NO release (peak NO concentration) from RAECs was significantly less than that in the absence of L-NAME (0.18 +/- 0.04 microM vs 0.47 +/- 0.13; P < 0.01). After removal of L-NAME, NO release partially recovered (0.39 +/- 0.10 microM). In conclusion, the microcoaxial electrode was successfully applied to direct and continuous NO measurement in biological systems. 相似文献
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A friction stir processing (FSP) technique has been developed for use with aluminum and magnesium alloys, with the goal of high-strain-rate processing. In this study, we treat the microstructures of aluminum samples, of three levels of purity, manipulating their grain sizes, making them finer, using FSP. Grain sizes decreased with an increase in the Zener-Hollomon parameter, at all purity levels. However, the recrystallized grain of FSP-ed ultra-high-purity (99.999%) aluminum was particularly large, compared to the grain sizes of lower-purity (99% and 99.99%) aluminum, when subjected to the same processing conditions. The grain size reached a certain minimum value at high-strain-rates of processing, which we report for each purity level herein. 相似文献
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Min‐Jung Bae Takeshi Ishii Kanako Minoda Yukiko Kawada Tatsuya Ichikawa Taiki Mori Miya Kamihira Tsutomu Nakayama 《Molecular nutrition & food research》2009,53(6):709-715
(–)‐Epigallocatechin gallate (EGCg) is the major component of green tea and is known to show strong biological activity, although it can be easily oxidized under physiological conditions. In this study, we indicate that EGCg is stable in human serum and that human serum albumin (HSA) stabilizes EGCg under aerobic condition. Although EGCg is usually decomposed within 1 h in aqueous solution at neutral pH, EGCg in serum and phosphate buffer (pH 7.4) containing HSA was stable over 1 h, even at neutral and slightly alkaline pH. Under these conditions, EGCg binds to HSA non‐covalently. The sulfhydryl group acts as an antioxidant for EGCg oxidation. Incubation of EGCg with HSA is accompanied by the oxidation of a free sulfhydryl group in HSA. These results suggest that the antioxidant property and the binding capacity of HSA contribute to the stabilization of EGCg in human serum. 相似文献
9.
The largest revision of the building code in Japan was released in 1981, in which the examination of ultimate lateral strength of structure is required. As a result, the capacity of the earthquake resistance of buildings is different between those designed before and after the revision of the code.The objective of this paper is to evaluate the seismic reliability of two groups of reinforced concrete buildings; one is the building group designed by the current code (after the 1981 revision) and the other is the building group designed by the former code (before the revision). The site is assumed to be Sendai city, Japan. Seismic reliability analyses of buildings are carried out and the probability of structural responses exceeding certain response levels during 100 years is evaluated for each type of building group. In addition to the uncertainty of earthquake forces, the uncertainties of structural characteristics, such as lateral resistance and the first natural period of structures, are considered based on the statistics of existing buildings. 相似文献
10.
Haruhiko Morito Tomoyuki Ide Taiki Karahashi Hironori Orikasa Takahiro Yamada Hisanori Yamane 《Journal of the American Ceramic Society》2009,92(11):2578-2582
A bulk layer of aluminum nitride (AlN) polycrystals was synthesized on a boron nitride crucible surface by heating Al chunks with 5 mol% of bismuth at 1273 K for 3 h under NH3 gas flow. The fragments of the layer were characterized by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The platelet grains of AlN with a size of 0.1–1.0 μm and having preferred orientation of the c -axis perpendicular to the layer were formed at the crucible side. Nanotubes 6–15 μm long and about 20–100 nm thick grew on the gas phase side of the layer. 相似文献