Gold nanoparticles-coated eggshell membrane with immobilized glucose oxidase for fabrication of glucose biosensor

This work reports the fabrication and application of a glucose biosensor based on the catalytic effect of gold nanoparticles (AuNPs) on enzymatic reaction for blood glucose determination. AuNPs were initially in situ synthesized on the surface of an eggshell membrane (ESM) which was subsequently immobilized with glucose oxidase (GO_x) to produce a GO_x-AuNPs/ESM. The GO_x-AuNPs/ESM was positioned on the surface of an oxygen electrode to form a GO_x-AuNPs/ESM glucose biosensor. The effects of pH, concentration of phosphate buffer solution and amount of GO_x on the response of the GO_x-AuNPs/ESM glucose biosensor were studied in detail. AuNPs on GO_x/ESM can improve the calibration sensitivity (30% higher than GO_x/ESM without AuNPs), stability (87.3% of its initial response to glucose after 10-week storage) and shortens the response time (<30 s) of the glucose biosensor. The linear working range for the GO_x-AuNPs/ESM glucose biosensor is 8.33 μM to 0.966 mM glucose with a detection limit of 3.50 μM (S/N = 3). The biosensor has been successfully applied to determine the glucose in human blood serum samples and the results compared well to a standard spectrophotometric method commonly used in hospitals. Our work demonstrates that the developed GO_x-AuNPs/ESM glucose biosensor has potential in biomedical analysis.     

工程陶瓷小孔超声振动钻削的理论探讨

通过对超声振动钻削的运动学分析 ,推导了临界钻削速度的计算公式 ,并分析了超声振动钻削提高孔径质量的机理。     

数控机床铣削等距型面轴的理论研究

提出了在数控机床上铣削等距型面轴的新方法。通过建立和分析等距曲面及其加工中有关问题的数学模型，得到了数控铣削等距型面轴时确定自适应步长的算法以及行距、残留高度等重要理论问题的计算公式，为等距型面轴数控加工的实验研究奠定了理论基础。     

转炉钢铁料消耗高的原因分析及措施

针对转炉钢铁料消耗高等问题从技术、管理与操作、原辅料、机器、测量等6个方面找出了造成钢铁料消耗高的30多个因素，并利用打分法及专家咨询法找出了5个优先解决的问题，分析了具体原因并提出了相应的解决办法。     

实用三弧段等距型面轴的数控铣削仿真研究

从分析实用等距曲线的构成入手,推导出实用三弧段等距型面的大圆弧半径计算公式和型面曲线的数学模型,应用M asterCAM软件进行二维图形的建模,并进行数控铣削仿真,最后生成NC数控加工程序。     

长输原油管道站场总图设计部分难点辨析——以仪长复线安庆输油站为例

以仪长复线安庆输油末站为例,从站场总图专业设计角度所遇到的几个关键性的问题入手,结合实际逐一对其进行概念性的辨析,并进行归纳总结,以求从中得出有益经验,为日后工程建设提供了良好的借鉴。     

钙基脱硫剂盛钢桶内脱硫的应用

通过对盛钢桶加入钙基脱硫剂后钢水和钢水渣中的硫含量分析,总结出加入钙基脱硫剂的实用性.实践证明,无论在硫含量高低的情况下加入钙基脱硫剂都对钢水减少硫含量和抑制回硫有显著的效果.     

Electrogenerated chemiluminescence behavior of graphite-like carbon nitride and its application in selective sensing Cu2+

This paper reports for the first time the electrogenerated chemiluminescence (ECL) behavior of graphite-like carbon nitride (g-C(3)N(4)) with K(2)S(2)O(8) as the coreactant. The possible ECL reaction mechanisms are proposed. The spectral features of the ECL emission and photoluminescence (PL) of g-C(3)N(4) are compared, and their resemblance demonstrates that the excited states of g-C(3)N(4) from both ECL and photoexcitation are the same. The effects of K(2)S(2)O(8) concentration, pH, g-C(3)N(4)/carbon powder ratio, and scan rate on the ECL intensity have been studied in detail. Furthermore, it is observed that the ECL intensity is efficiently quenched by trace amounts of Cu(2+). g-C(3)N(4) is thus employed to fabricate an ECL sensor which shows high selectivity to Cu(2+) determination. The limit of detection is determined as 0.9 nM. It is anticipated that g-C(3)N(4) could be a new class of promising material for fabricating ECL sensors.     

一种用于双输入双输出液位控制系统的状态估计控制器的设计

设计一种用于双输入双输出液位控制系统的状态估计控制器。从双输入双输出液位控制系统的结构出发,研究其结构及工作原理。在考虑系统集中扰动的基础上,利用系统的已知增益时滞比求取系统的状态空间方程。采用线性扩张状态观测器,构造系统的估计方程,并通过期望信号与输出信号误差的比值,构建状态估计控制器。通过所设计的状态估计控制器,求取双输入双输出液位系统中两个通道的估计方程以及控制信号。利用干扰观测器方法与所提方法分别对恒定及变化期望液位曲线进行跟踪。结果表明:在跟踪恒定及变化液位曲线时,所提方法对双输入双输出液位系统的控制效果较好,能够控制系统液位快速按照期望液位变化,可实现对双输入双输出液位的准确控制。     

炮采工作面攉煤机主参数的理论计算

针对我国目前炮采工作面装煤的现状，设计出了一种新型便携式炮采工作面攉煤机，对实现炮采工作面装煤机械化有促进作用。并论述了该机的工作原理，推导了装煤能力和装煤率以及刮板的运行阻力的理论计算公式。