Pharmacokinetic and bioequivalence study of two gemfibrozil preparations

A comparative pharmacokinetic study has been performed in 19 healthy male volunteers in a single-dose, randomized, two way cross-over design with two preparations of gemfibrozil (CAS 25812-30-0) capsules each of them containing 300 mg active ingredient. The test preparation was Innogem 300 mg capsule. The plasma concentration of gemfibrozil was determined by a validated HPLC-UV analytical method. The statistical comparison of individual pharmacokinetic parameters (AUC0-16, AUC0-oc Cmax, tmax) of the two capsule preparations was performed by three-way analysis of variance (ANOVA), Wilcoxon's, Westlake's, Schuirmann's and Hanck-Anderson's method as well as by the calculation of confidence intervals on the ratio of test/reference. The relative bioavailability of the test preparation with respect to the reference preparation in terms of the AUC0-oc was 104.06 +/- 21.61%. No statistically significant difference was found between the pharmacokinetic parameters, calculated from plasma concentration-time curves, indicating that the two preparations were bioequivalent.     

Retailoring Box Splines to Lattices for Highly Isotropic Volume Representations

3D box splines are defined by convolving a 1D box function with itself along different directions. In volume visualization, box splines are mainly used as reconstruction kernels that are easy to adapt to various sampling lattices, such as the Cartesian Cubic (CC), Body‐Centered Cubic (BCC), and Face‐Centered Cubic (FCC) lattices. The usual way of tailoring a box spline to a specific lattice is to span the box spline by exactly those principal directions that span the lattice itself. However, in this case, the preferred directions of the box spline and the lattice are the same, amplifying the anisotropic effects of each other. This leads to an anisotropic volume representation with strongly preferred directions. Therefore, in this paper, we retailor box splines to lattices such that the sets of vectors that span the box spline and the lattice are disjoint sets. As the preferred directions of the box spline and the lattice compensate each other, a more isotropic volume representation can be achieved. We demonstrate this by comparing different combinations of box splines and lattices concerning their anisotropic behavior in tomographic reconstruction and volume visualization.     

A New Well-Solvable Class of PNS Problems

A manufacturing system consists of operating units which convert their input materials into their output materials. In the problem of designing a process network, we have to find a suitable network of operating units which produces the desired products from the given raw materials. If we consider this process network design problem from a structural point of view, then we obtain a combinatorial optimization problem called the Process Network Synthesis or (PNS) problem. It is known that the PNS problem is NP-complete. Here, a new method is presented to reduce the solution of some more difficult PNS problems to the solution of simpler ones, and using this method, a new well-solvable class of PNS problems is established. Received February 12, 1999; revised October 24, 2000     

Investigation of impact materials around Barringer Meteor Crater by SEM-EDX and micro-PIXE techniques

Impact materials collected at the Barringer Meteor Crater have been characterized by SEM-EDX and micro-PIXE techniques. Fine textural and true elemental images were created. As a main feature silica-bearing shell and an S–Fe–Ni–Cu core could be distinguished. Three different types of S–Fe–Ni–Cu systems were identified such as chalcopyrite, pentlandite and pyrrhotite.     

Distribution-free uncertainty quantification for kernel methods by gradient perturbations

Machine Learning - We propose a data-driven approach to quantify the uncertainty of models constructed by kernel methods. Our approach minimizes the needed distributional assumptions, hence,...     

Microfluidic-Generated Seeds for Gold Nanotriangle Synthesis in Three or Two Steps

Nanoparticle synthesis has drawn great attention in the last decades. The study of crystal growth mechanisms and optimization of the existing methods lead to the increasing accessibility of nanomaterials, such as gold nanotriangles which have great potential in the fields of plasmonics and catalysis. To form such structures, a careful balance of reaction parameters has to be maintained. Herein, a novel synthesis of gold nanotriangles from seeds derived with a micromixer, which provides a highly efficient mixing and simple parameter control is reported. The impact of the implemented reactor on the primary seed characteristics is investigated. The following growth steps are studied to reveal the phenomena affecting the shape yield. The use of microfluidic seeds led to the formation of well-defined triangles with a narrower size distribution compared to the entirely conventional batch synthesis. A shortened two-step procedure for the formation of triangles directly from primary seeds, granting an express but robust synthesis is further described. Moreover, the need for a thorough study of seed crystallinity depending on the synthesis conditions, which – together with additional parameter optimization – will bring a new perspective to the use of micromixers which are promising for scaling up nanomaterial production is highlighted.     

Time course of vinblastine-induced autophagocytosis and changes in the endoplasmic reticulum in murine pancreatic acinar cells: a morphometric and biochemical study

The time course of the vinblastine(-sulfate; 10 mg/kg body weight, single injection)-induced enlargement and subsequent regression of the autolysosomal compartment was studied by electron microscopic morphometrical and cell biochemical methods in order to gain information concerning some key problems of this major route of intralysosomal degradation of the cell's endogenous macromolecules and structures. Detailed analysis of the dynamics of the total autophagic vacuole (AV) compartment and its different subcompartments (early, advanced, late, and fused AVs), as well as of changes of rough-surfaced endoplasmic reticulum (RER) showed: 1. Pancreatic acinar cells react to vinblastine biphasically, i.e. two expansion phases of the AV compartment, the first in the 0 to 90 min and the second in the 2 to 8 h post-injectional periods, were detected. 2. Fusions of AVs are not inhibited by vinblastine, at least during the second expansion phase when cytoplasmic volume fraction (CVF) of fused AVs steadily increased until the 12th h. Fusion of early, advanced and late AVs or composition of fused complex vacuoles (AVc) are somehow regulated, as the proportion of the three AV stages from the CVF of AVc, was maintained constant throughout the second expansion phase. 3. Stimulation of autophagosome formation and resulting substrate overload seems to be the primary mode of action by which vinblastine causes the enormous expansion of the autolysosomal compartment. 4. Degranulation of the rough-surfaced endoplasmic reticulum (RER) membranes occurs in a biphasic fashion, similarly to the volume and surface changes of the AV compartment, thus supporting our previous hypothesis, that labilization or change of RER may have a role in the formation of autophagosomes. 5. Vinblastine-induced autophagocytosis is a selective process, as mitochondria, Golgi elements and zymogen granules are very much underrepresented, whereas RER is more than twice overrepresented in the volume of early AVs, when compared to their volume fraction in the whole cytoplasm. 6. Immunogold electron microscopy revealed the presence of ubiquitinylated proteins in advanced and late, but not in early AVs.     

Mathematical Modeling of Drying Processes Using Methods of the Nonequilibrium Thermodynamics and Percolation Theory

A twofold refinement of the basic mathematical model for describing a coupled heat and mass transfer taking place in porous media is presented. The common application of irreversible thermodynamics and fluctuation theory of phase transitions is proposed for calculating the moisture level and temperature. Instead of parabolic partial differential equations, hyperbolic type partial differential equations are used. The relaxation time constants, whose percolation state-dependence is also taken into account, are incorporated into this formalism. Some possible new research domains in mathematical and statistical physics are also indicated.     

Selective formation of tungsten nanowires

We report on a process for fabricating self-aligned tungsten (W) nanowires with polycrystalline silicon core. Tungsten nanowires as thin as 10 nm were formed by utilizing polysilicon sidewall transfer technology followed by selective deposition of tungsten by chemical vapor deposition (CVD) using WF6 as the precursor. With selective CVD, the process is self-limiting whereby the tungsten formation is confined to the polysilicon regions; hence, the nanowires are formed without the need for lithography or for additional processing. The fabricated tungsten nanowires were observed to be perfectly aligned, showing 100% selectivity to polysilicon and can be made to be electrically isolated from one another. The electrical conductivity of the nanowires was characterized to determine the effect of its physical dimensions. The conductivity for the tungsten nanowires were found to be 40% higher when compared to doped polysilicon nanowires of similar dimensions.     

Noncovalently bonded quinone on self-assembled molecular layers

Aminoalkanethiol surface modifiers were investigated on gold electrodes. It was established, that quinone molecules could be attached to these surface layers in three different forms with well-distinguished redox potentials. Chemical reaction with the amino groups of the modifiers could produce monosubstituted and disubstituted quinones attached covalently to the layers. However, a third form could be detected, as well that seems to be quinone attached non-covalently to the monosubstituted quinone molecules.