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LE Lindner 《Canadian Metallurgical Quarterly》1993,41(3):439-445
The silver-staining technique for nucleolar organizer regions (AgNOR) of Ploton et al., as popularized by Crocker, is being widely used for evaluation of nucleolar function, especially in neoplasia. It suffers from limited reliability, background staining, precipitates, and fading of the sections. Factors were identified that affect these problems. The oxidation-reduction level and gelatin used are particularly important. An improved procedure is presented which incorporates pre-reduction of the sections, selection of an optimal gelatin, and post-treatment of the sections to produce a permanent preparation. It is compatible with many fixatives and with other stains used before or after the silver stain. 相似文献
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R. Breil T. Fries J. Garnaes J. Haycocks D. Hüser J. Joergensen W. Kautek L. Koenders N. Kofod K. R. Koops R. Korntner B. Lindner W. Mirand A. Neubauer J. Peltonen G. B. Picotto M. Pisani H. Rothe M. Sahre M. Stedman G. Wilkening 《Precision Engineering》2002,26(3)
Comparison measurements on reference standards are reported in which 13 partners with different instruments took part. A set of prototype standards which had been produced and calibrated within a European project were used for the measurements. Here, results of measurements on a 240 nm step height standard and a two-dimensional lateral standard with a nominal pitch of 1 μm are reported. 相似文献
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Sabine Timpf 《Informatik-Spektrum》2008,33(5):70-74
Im Zeitalter der Informationsgesellschaft, so wurde postuliert, spielten r?umliche Distanzen keine Rolle mehr und unsere r?umliche
Mobilit?t n?hme ab. Die Mobilit?t in den letzten Jahren, speziell die Freizeitmobilit?t, hat hingegen zugenommen. Die Bereitstellung
ortsbezogener Dienste – ,,Location-based Services“ – unterstützt und f?rdert dieses Verhalten. 相似文献
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David Alleysson Sabine Süsstrunk Jeanny Hérault 《IEEE transactions on image processing》2005,14(4):439-449
There is an analogy between single-chip color cameras and the human visual system in that these two systems acquire only one limited wavelength sensitivity band per spatial location. We have exploited this analogy, defining a model that characterizes a one-color per spatial position image as a coding into luminance and chrominance of the corresponding three colors per spatial position image. Luminance is defined with full spatial resolution while chrominance contains subsampled opponent colors. Moreover, luminance and chrominance follow a particular arrangement in the Fourier domain, allowing for demosaicing by spatial frequency filtering. This model shows that visual artifacts after demosaicing are due to aliasing between luminance and chrominance and could be solved using a preprocessing filter. This approach also gives new insights for the representation of single-color per spatial location images and enables formal and controllable procedures to design demosaicing algorithms that perform well compared to concurrent approaches, as demonstrated by experiments. 相似文献
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Mirna Suárez-Quiroz Béatrice De Louise Oscar Gonzalez-Rios Michel Barel Bernard Guyot Sabine Schorr-Galindo & Joseph-Pierre Guiraud 《International Journal of Food Science & Technology》2005,40(6):605-611
Roasting coffee led to a drop in the ochratoxin A (OTA) concentration, as measured by the reference method, especially for dark type roasts. The way the beverage was prepared also affected the OTA content, which could paradoxically be higher than that of the initial roasted coffee. Assays on the thermal stability of pure OTA showed that it ought to be found in larger quantities in roasted coffee. This suggested that OTA was masked by reactions with the substrate during roasting. The absence of OTA in green coffee is therefore the best guarantee of safety. 相似文献
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Karin Metz Stephanie Fl?ter Christoph Kr?ger Carolin Donath Daniela Piontek Sabine Gradl 《Nicotine & tobacco research》2007,9(8):853-863
Smokers with smoking-related diseases who are hospitalized in rehabilitation centers should be offered smoking cessation. This is the first study evaluating whether telephone booster sessions after intensive inpatient treatment are an effective strategy. The present study was conducted in 13 rehabilitation centers for somatic disorders as a prospective multicenter study with a randomized treatment-control group design. We compared abstinence rates after hospital discharge from treatment that included a group smoking cessation program with (treatment group) and without telephone booster sessions (control group). Data from 290 smokers were analyzed. After 6 and 12 months the treatment group achieved abstinence rates twice as high as those of the control group. Men profited more from telephone booster sessions than did women. Results indicated that telephone booster sessions were highly effective (even) after an inherently intensive group program during a hospital stay. Further research should focus on the special needs of women receiving telephone counseling. 相似文献
9.
Dr. Sabine Wicker Prof. Dr. Holger Rabenau Priv.-Doz. Dr. Dr. Rene Gottschalk 《Ethik in der Medizin》2007,19(3):216-218
Ohne Zusammenfassung 相似文献
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