Human herpesvirus type 6 DNA derived from human breath was discovered to contaminate PCR reactions during routine reaction preparation. Parallel PCR experiments were conducted in which expiratory secretions were blocked by a surgical mask, while others were performed without any attempt to circumvent respiratory contamination. The experimenter was previously determined to harbor HHV-6 DNA in the saliva. All reactions in which expiration was obstructed were negative for HHV-6 DNA via PCR. Reactions in which there was no attempt to obstruct respiratory secretions were positive for HHV-6 DNA. These data suggest that PCR assays investigating the presence of HHV-6 may be highly susceptible to contamination from the experimenter leading to false positive results. 相似文献
For an autonomous system to perform maintenance tasks in a networking device or a radio base station (RBS), it has to deal with a series of technological challenges ranging from identifying hardware-related problems to manipulating connectors. This paper describes the development of a robot maintenance system dedicated to detect and resolve faulty links caused by unplugged or poorly connected cables. Although the maintenance system relies on four subsystems, we significantly focus on our low-cost and efficient custom gripper solution developed to handle RJ45 Ethernet connectors. To examine our gripper, we conducted three experiments. First, a qualitative questionnaire was submitted to 30 users in the case of the teleoperated scenario of the gripper attached to a robotic arm. Similarly, we also tested the automatic operation mode. The results showed that our system is reliable and delivers a highly efficient maintenance tool in both teleoperated and autonomous operation modes. The practical experiment containing the removal or unplugging of connectors demonstrated our gripper’s ability to adequately handle these, whereas the feedback from the questionnaire pointed to a positive user experience. The automatic test assessed the gripper’s robustness against the continuous operation.
The network function virtualization (NFV) paradigm replaces hardware-dependent network functions by virtual network functions (VNFs) that can be deployed in commodity hardware, including legacy servers. Consequently, the use of NFV is expected to reduce operating and capital expenses, as well as improve service deployment operation and management flexibility. For many use cases, the VNFs must be visited and invoked following a specific order of execution in order to compose a complete network service, named service function chain (SFC). Nonetheless, despite the benefits from NFV and SFC virtualization technologies, their introduction must not harm network performance and service availability. On the one hand, redundancy is seen by network service planners as a mechanism well established to combat availability issues. At same time, there is a goal to optimize resource utilization in order to reduce operational expenditure. In this article, we share our experience in the design use of a framework, named SPIDER, focused on SFC placement that considers the network infrastructure condition and the required SFC availability to define the placement strategy. The SPIDER monitors the status of infrastructure nodes and links and defines which servers the VNFs should be placed on and the number of redundant replicas needed. We present a proof-of-concept of SPIDER using Kubernetes to launch the VNFs as containers. We also use Kubernetes to forward the traffic between the VNFs, composing the service chain. We perform experiments to evaluate the runtime of SPIDER and the SFC delay under different network conditions. 相似文献
A country’s economy heavily depends on agricultural development. However, due to several plant diseases, crop growth rate and quality are highly suffered. Accurate identification of these diseases via a manual procedure is very challenging and time-consuming because of the deficiency of domain experts and low-contrast information. Therefore, the agricultural management system is searching for an automatic early disease detection technique. To this end, an efficient and lightweight Deep Learning (DL)-based framework (E-GreenNet) is proposed to overcome these problems and precisely classify the various diseases. In the end-to-end architecture, a MobileNetV3Small model is utilized as a backbone that generates refined, discriminative, and prominent features. Moreover, the proposed model is trained over the PlantVillage (PV), Data Repository of Leaf Images (DRLI), and a new Plant Composite (PC) dataset individually, and later on test samples, its actual performance is evaluated. After extensive experimental analysis, the proposed model obtained 1.00%, 0.96% and 0.99% accuracies on all three included datasets. Moreover, the proposed method achieves better inference speed when compared with other State-Of-The-Art (SOTA) approaches. In addition, a comparative analysis is conducted where the proposed strategy shows tremendous discriminative scores as compared to the various pre-trained models and other Machine Learning (ML) and DL methods. 相似文献
Quality function deployment (QFD) is a customer-oriented design tool for developing new or improved products to achieve higher customer satisfaction by integrating various functions of an organization. The engineering characteristics (ECs) affecting the product performances are designed to match the customer attributes (CAs). However, from the viewpoint of the QFD team, product design processes are performed in imprecise environments, and more than one factor must be taken into account in determining the target levels of ECs, especially the limited resources and increased market competition. This paper presents an imprecise goal programming (GP) approach to determine the optimum target levels of ECs in QFD for maximizing customer satisfaction under resource limitation and considerations of market competition. Based on benchmarking data of CAs, the concept of satisfaction functions is utilized to formulate explicitly the customer's preferences and to integrate the competitive analysis of target market into the modelling and solution process. In addition, the relationships linking CAs and ECs and the ECs to each other are integrated by functional relationships. The proposed approach will be illustrated through a car door design example. 相似文献
Virtual Worlds (VW), such as Massive Multiplayer Online Social Games, have been gaining increasing attention in the last few
years, mainly due to the new way users interact with them. However, little effort has been devoted to understand their traffic
profile and the implications to the traffic management area. With the current growing rate of VWs’ usage, their traffic demand
could eventually impose a significant burden on the operation of a typical Internet Service Provider (ISP) network. In this
paper, we seek to understand the traffic behavior of an increasingly popular VW application, namely Second Life (SL), from
both the connection and network level perspectives. We also show results of a traffic analysis of a SL client, when an avatar
performs different actions in the VW, at different places and under different network conditions. Our results show that SL
makes intensive use of network resources (mostly bandwidth), since the capacity needed for having a full SL experience (listening
to live music) may reach 500 kbps. We then extend the traffic analysis work on SL by providing analytical models for its traffic
profile. We aim at helping the network management and planning area to estimate the impact of an intense use of such VW on
access links or core networks. Such synthetic models are also useful to the networking research community for the use in network
simulations. 相似文献
This paper addresses the synthesis of Petri net (PN) controller for the forbidden state transition problem with a new utilisation of the theory of regions. Moreover, as any method of control synthesis based on a reachability graph, the theory of regions suffers from the combinatorial explosion problem. The proposed work minimises the number of equations in the linear system of theory of regions and therefore one can reduce the computation time. In this paper, two different approaches are proposed to select minimal cuts in the reachability graph in order to synthesise a PN controller. Thanks to a switch from one cut to another, one can activate and deactivate the corresponding?PNcontroller. An application is implemented in a flexible manufacturing system to illustrate the present method. Finally, comparison with previous works with experimental results in obtaining a maximally permissive controller is presented. 相似文献
Associative classification has been shown to provide interesting results whenever of use to classify data. With the increasing complexity of new databases, retrieving valuable information and classifying incoming data is becoming a thriving and compelling issue. The evidential database is a new type of database that represents imprecision and uncertainty. In this respect, extracting pertinent information such as frequent patterns and association rules is of paramount importance task. In this work, we tackle the problem of pertinent information extraction from an evidential database. A new data mining approach, denoted EDMA, is introduced that extracts frequent patterns overcoming the limits of pioneering works of the literature. A new classifier based on evidential association rules is thus introduced. The obtained association rules, as well as their respective confidence values, are studied and weighted with respect to their relevance. The proposed methods are thoroughly experimented on several synthetic evidential databases and showed performance improvement. 相似文献