Cytoskeleton Elements Contribute to Prion Peptide-Induced Endothelial Barrier Breakdown in a Blood–Brain Barrier In Vitro System

The mechanisms involved in the interaction of PrP 106-126, a peptide corresponding to the prion protein amyloidogenic region, with the blood–brain barrier (BBB) were studied. PrP 106-126 treatment that was previously shown to impair BBB function, reduced cAMP levels in cultured brain endothelial cells, increased nitric oxide (NO) levels, and changed the activation mode of the small GTPases Rac1 (inactivation) and RhoA (activation). The latter are well established regulators of endothelial barrier properties that act via cytoskeletal elements. Indeed, liquid chromatography-mass spectrometry (LC-MS)-based proteomic profiling study revealed extensive changes in expression of cytoskeleton-related proteins. These results shed light on the nature of the interaction between the prion peptide PrP 106-126 and the BBB and emphasize the importance of the cytoskeleton in endothelium response to prion- induced stress.     

Advances in the Structural Strategies of the Self-Assembly of Photoresponsive Supramolecular Systems

Photosensitive supramolecular systems have garnered attention due to their potential to catalyze highly specific tasks through structural changes triggered by a light stimulus. The tunability of their chemical structure and charge transfer properties provides opportunities for designing and developing smart materials for multidisciplinary applications. This review focuses on the approaches reported in the literature for tailoring properties of the photosensitive supramolecular systems, including MOFs, MOPs, and HOFs. We discuss relevant aspects regarding their chemical structure, action mechanisms, design principles, applications, and future perspectives.     

Assessment of durability of recycled aggregate concrete produced by two-stage mixing approach

As more than 50% construction and demolition (C&D) wastes are composed of concrete debris in Hong Kong, recycling this debris into Recycled Aggregate (RA) for production of Recycled Aggregate Concrete (RAC) is an efficient way to alleviate the burden on landfill areas. Since RA is generated from concrete debris which has undergone years of services, the resulting RAC bears the weaknesses of lower density, higher water absorption, and higher porosity that limit them to lower-grade applications. Pinpointing to these weaknesses, Tam et al. [2005, Cement Concrete Res 35(6):1195–1203] developed the Two-Stage Mixing Approach (TSMA) for improving the strength of RAC, leading to the possibility in applying RAC for higher-grade applications. While the improvement in strength by TSMA has been proven in Tam et al.’s work [2005, Cement Concrete Res 35(6):1195–1203], the durability, in terms of deformation (shrinkage and creep) and permeability (water, air and chloride permeability), remains to be verified. In this paper, 0%, 20% and 100% of RA substitutions have been experimented to compare the durability performance of the Normal Mixing Approach (NMA) and the TSMA. Experiment results highlight that: (i) the higher the substitutions of RA, the weaker the performance of RAC; and (ii) the deformation and permeability of RAC can be enhanced when adopting TSMA. Therefore, it demonstrates that TSMA can help to improve the durability of RAC, on top of the previously verified strength improvement, and thus opening up wider applications of RAC.     

Eine Echtzeit-Online-Methode zur Beurteilung der künstlichen Grundwasseranreicherung und Grundwasserentnahme

Grundwasser - Eine nachhaltige Wasserversorgung in urbanen Gebieten stellt aufgrund des Nutzungsdrucks sowie vielfältiger anthropogener Einträge eine große Herausforderung dar....     

Behaviors of recycled aggregate concrete-filled steel tubular columns under eccentric loadings

The paper investigates the behaviors of recycled aggregate concrete-filled steel tubular (RACFST) columns under eccentric loadings with the incorporation of expansive agents. A total of 16 RACFST columns were tested in this study. The main parameters varied in this study are recycled coarse aggregate replacement percentages (0%, 30%, 50%, 70%, and 100%), expansive agent dosages (0%, 8%, and 15%) and an eccentric distance of compressive load from the center of the column (0 and 40 mm). Experimental results showed that the ultimate stresses of RACFST columns decreased with increasing recycled coarse aggregate replacement percentages but appropriate expansive agent dosages can reduce the decrement; the incorporation of expansive agent decreased the ultimate stresses of RACFST columns but an appropriate dosage can increase the deformation ability. The recycled coarse aggregate replacement percentages have limited influence on the ultimate stresses of the RACFST columns and has more effect than that of the normal aggregate concrete-filled steel tubular columns.     

Evaluations of existing waste recycling methods: A Hong Kong study

Environmental problems have been considered as a serious situation in Hong Kong construction. Waste management is pressing harder with the alarming signal warning the industry. Reuse, recycling and reducing the wastes are considered as the only methods to recover the wastes generated; however, the implementations still have much room for improvement. In order to ameliorate the existing situations, evaluations of the existing waste recycling methods are studied in this research. A telephone interviewing to the recyclers, site visits to the construction and demolition sites (including the Lower Ngau Tau Kok Estate Phase 1) and the centralized recycling plant in Tuen Mun Area 38 are under investigation. Difficulties encountered for various recycling parties are investigated. Rather than the poor quality found from the recyclable materials, they found the high investment cost, lengthy demolition period and limited space caused the major barriers for them. Therefore, some recommendations are suggested: (i) proposing a higher landfill charging scheme; (ii) setting up a centralized centre for recycling the materials; (iii) examining the Hong Kong government should be supported in the provision of land for recycling plants; (iv) implementing innovative demolition methods; (v) allowing some locations in town for residents’ easy access to drop-off recyclable materials; (vi) allowing flexible demolition periods; (vii) setting up recycling plant in town or in the form of mobile installations; (viii) reusing the reusable components as donations to the charity organization; (ix) providing higher flexibility in receiving concrete waste in Tuen Mun Area 38 recycling plant; and (x) balancing the supply and demand of recycled materials through legislations or incentive schemes.     

A web‐based performance assessment system for environmental protection: WePass

In response to the increasing public concern over the effects of construction waste on the environment, a performance assessment system has been designed to provide an instant, online assessment of how well a construction site performs environmentally, e.g. measuring the level of pollution generated from a construction site. Through the use of both Internet and database technologies, it is possible to collect and present data that are essential for environmental management online. To achieve this, a set of environmental performance parameters was derived through a postal questionnaire survey and interviews with experts in the field. A conceptual framework was developed, of which the key components were: template, knowledge base, output data and benchmark group. In combination, these factors provide a system that enables speedy assessment and effective management of environmental performance on construction sites, hence prompt decisions can be made and corrective actions can be taken before potential hazards turn into real disasters.     

An Investigation into Lubrication and Oxide Breakdown During Load-Carrying Capacity Testing

Changes occurring in the friction and wear mechanisms during a load-carrying capacity test, lubricated with cetane containing a carboxylic acid, were investigated. The changes in wear scar/track appearance and oxide coverage/composition were analyzed during every load stage.

The main conclusions were as follows:

• The breakdown in the protective oxide layer formed on the opposing steel surfaces was found to be the prerequisite for initiation of seizure.

• The seizure load achieved during load-carrying capacity testing quantifies the ability of the test fluid to prevent transition to the adhesive wear regime.

• The most severe surface damage was found to occur during the first few seconds after test initiation. Desorption of the adsorbed lubricant film and the subsequent removal of the naturally occurring thin oxide layer results in the initial period of adhesive wear.

• Partial recovery to a state of acceptable friction occurs after the period of initial seizure. During this period, the surface coverage by the adsorption lubricant molecules and the oxide coverage are sufficient to prevent severe adhesive wear from occurring. Wear is primarily a combination of oxidative, abrasive, and fatigue wear (all possible in the regions of mixed friction and boundary lubrication).

• Final lubricant breakdown and eventual seizure are initiated when the oxide removal rate exceeds the oxide formation rate resulting in severe adhesive wear followed by seizure.

     

The dance of molecules: new dynamical perspectives on highly excited molecular vibrations

At low energies, molecular vibrational motion is described by the normal modes model. This model breaks down at higher energy, with strong coupling between normal modes and onset of chaotic dynamics. New anharmonic modes are born in bifurcations, or branchings of the normal modes. Knowledge of these new modes is obtained through the window of frequency-domain spectroscopy, using techniques of nonlinear classical dynamics. It may soon be possible to "watch" molecular rearrangement reactions spectroscopically. Connections are being made with reaction rate theories, condensed phase systems, and motions of electrons in quantum dots.     

Diminished macrophage cholesterol removal rate by the altered HDL metabolism in the Nagase analbuminemic rat

Dyslipoproteinemia of the Nagase analbuminemic rat (NAR) is characterized by elevated concentrations of VLDL and LDL attributed to increased rates of liver lipoprotein synthesis. Increased lysophosphatidylcholine (LPC) in NAR HDL has been attributed to high plasma LCAT activity. We show here that, as compared with Sprague-Dawley rats (SDR), NAR plasma triacylglycerol (TAG), total cholesterol (TC), HDL TAG, protein, total phospholipids (PL), LPC, and PS are increased. These alterations rendered the NAR HDL particle more susceptible to the activity of the enzyme hepatic lipoprotein lipase (HL), which otherwise was unaltered in our study. Fractional catabolic rates in blood of the autologous ¹²⁵I-apoHDL (median and lower quartile values), were, respectively, 0.231 and 1.645 (n=10) in NAR as compared with 0.140 and 0.109 (n=10) in SDR (P=0.012), corresponding to synthesis rates of HDL protein of 89.8±33.7 mg/d in NAR and 17.4±6.5 mg/d in SDR (P=0.0122). Furthermore, Swiss mouse macrophage free-cholesterol (FC) efflux rates, measured as the percent [¹⁴C]-cholesterol efflux/6 h, were 8.2±2.3 (n=9) in NAR HDL and 11.2±3.2 (n=10) in SDR HDL (P=0.03). Therefore, in NAR the modification of the HDL composition slows down the cell FC efflux rate, and together with the increased rate of plasma HDL metabolism influences the reverse cholesterol transport system.