In-utero transplantation of parental CD34 haematopoietic progenitor cells in a patient with X-linked severe combined immunodeficiency (SCIDXI)

BACKGROUND: X-linked severe combined immunodeficiency (SCIDXI) is an inherited immune defect which leads to death in infancy from severe infections. The defect is caused by mutations of the IL-2RG gene that encodes for the common gamma chain shared by several cytokine receptors. The disease is characterised by lack of T and NK cells with normal numbers of B cells. SCIDXI can be cured by bone marrow transplantation (BMT) or prevented by abortion after prenatal diagnosis. METHODS: A male fetus was diagnosed as having SCIDXI by molecular, immunophenotypic, and functional analyses. The fetus was injected intraperitoneally under ultrasound guidance with CD34 haematopoietic progenitor cells purified from paternal bone marrow and T-cell depleted by E rosetting. Chimerism analysis was by HLA-DQ alpha typing and gamma-chain staining on cord blood. FINDINGS: A healthy 3.6 kg boy was delivered by caesarean section at 38 weeks of gestation with no clinical or laboratory signs of graft-versus-host disease. Engraftment of donor-derived CD2 cells was found at birth. At 3.5 months of age the infant is well and his T-cell counts and function are normal. INTERPRETATION: In-utero transplantation of haematopoietic progenitor cells allowed immune reconstitution of a fetus with SCIDXI and may be an alternative to elective abortion. Our report should encourage applications of this method to other inherited disorders curable by BMT.     

Iodine-123 labelled nor-beta-CIT binds to the serotonin transporter in vivo as assessed by biodistribution studies in rats

Flavobacterium aurantiacum NRRL B-184 possesses the ability to degrade aflatoxin B1 in solution and in several food items. Aflatoxin B1 is a potent carcinogen that causes significant economic losses to the agricultural and food industry. The role of trace metal ions (Cu2+, Mn2+, Zn2+, and Co2+) were studied in an effort to understand the enzymatic system involved in aflatoxin B1 degradation by F aurantiacum. The effect of divalent chelators (EDTA and 1,10-phenanthroline [OPT]) in the presence of the trace metal ions was studied as well. Aflatoxin B1 (10 microg/ml) was added to 72-h cultures of F aurantiacum that had been washed and resuspended in phosphate buffer (pH 7.0). HPLC was used to determine aflatoxin B1 concentration in these cultures. Incubating cells at 30 degrees C with 1 and 10 mM Cu2+, Mn2+, and Zn2+ significantly decreased aflatoxin B degradation after 4 and 24 h (P < 0.05). Decreased degradation was also observed with 1 and 10 mM Cu2+ and Zn2+ after 48 h and with 0.1 mM Cu2+ after 24 and 48 h. Co2+ did not have a significant effect on aflatoxin B1 degradation. EDTA and OPT did not counter the inhibition in the presence of Cu2+. The addition of 1 mM EDTA countered the inhibition by 1 mM Mn2+ after 4 and 24 h, but 1 mM OPT did not counter the inhibition by 10 mM Mn2+ after 4 and 24 h. OPT countered the inhibition by 1 mM Zn2+ after 4 and 48 h. These trace elements inhibit aflatoxin B1 degradation by F aurantiacum. In addition, their presence necessitates higher concentrations (>1 mM) of EDTA and OPT for the removal of their inhibitory effect.     

对DRAM结构中影响良率的缺陷进行监控

与上一代设计规则相比，亚100nm设计规则DRAM的开发和制造面临着前所未有的挑战。按照新型设计概念，由于器件结构不断缩小，其外形特征的深．宽比增大，这样就给工艺控制增加了难度。因此要求寻找一种能够对带状缺陷和孔洞缺陷进行可靠检测的高深．宽比（HAR）检测方法。另外，由于缺陷尺寸接近小型特征尺寸，许多从前认为不那么严重的缺陷如今变得极具损害力。因而缺陷检测和良率控制技术的改良成为加速开发并实现批量生产的基础。     

Hydrotope-Based Protocol to Determine Average Soil Moisture Over Large Areas for Satellite Calibration and Validation With Results From an Observation Campaign in the Volta Basin, West Africa

In West Africa, which is an extremely moisture-limited region, soil water information plays a vital role in hydrologic and meteorologic modeling for improved water resource planning and food security. Recent and upcoming satellite missions, such as SMOS and MetOp, hold promise for the regional observation of soil moisture. The resolution of the satellites is relatively coarse (>100 km²), which brings with it the need for large-scale soil moisture information for calibration and validation purposes. We put forward a soil moisture sampling protocol based on hydrotopes. Hydrotopes are defined as landscape units that show internally consistent hydrologic behavior. This hydrotope analysis helps in the following ways: 1) by ensuring statistically reliable validation via the reduction of the overall pixel variance and 2) by improving sampling schemes for ground truthing by reducing the chance of sampling bias. As a sample application, we present data from three locations with different moisture regimes within the Volta Basin during both dry and wet periods. Results show that different levels of reduction in the overall pixel variance of soil moisture are obtained, depending on the general moisture status. With respect to the distinction between the different hydrotope units, it is shown that under intermediate moisture conditions, the distinction between the different hydrotope units is highest, whereas extremely dry or wet conditions tend to have a homogenizing effect on the spatial soil moisture distribution. This paper confirms that well-defined hydrotope units yield an improvement at pixel-scale soil moisture averages that can easily be applied.     

Embedded HIMOS(R) flash memory in 0.35 μm and 0.25 μm CMOStechnologies

In this paper, the performance and reliability characteristics of the 0.35 μm/0.25 μm High Injection MOS (HIMIOS(R)) technology is described in detail. This flash EEPROM technology relies on source-side injection for programming and Fowler-Nordheim tunneling for erasing, and has been successfully implemented in a 1 Mbit memory array embedded in a 0.35 μm CMOS technology, adding only about 30% to the processing cost of digital CMOS. Due to its triple gate structure, the HIMOS(R) cell exhibits a high degree of flexibility and scalability. A fast programming operation (10 μs) at 3.3 V supply voltage is combined with an endurance of well over 100000 program/erase cycles, immunity to all possible disturb effects and a retention time that largely exceeds 100 years at 125°C. Furthermore, the cell has been scaled to a 0.25 μm version, which is a laterally scaled version with the same operating voltages and tunnel oxide thickness. The use of secondary impact ionization is investigated as well and proves to be very promising for future generations when the supply voltage is scaled below 2.5 V     

节约原料和提高效率的装置

在片梭和剑杆织机上采用了一种新型的织边装置极大地提高了织造效率和减少了织造时绞边的纱线浪费。这种装置也可改进后安装在旧织机上。