Buchbesprechungen

Ohne Zusammenfassung     

Dämpfen von Rundholz.—Erste Mitteilung: Papierchromatographische Untersuchungen an Dämpfkondensaten von Rotbuche

Ohne Zusammenfassung Mitteilung aus dem Forschungsinstitut für Holzwerkstoffe und Holzleime.     

Persisting deficits in rats "recovered" from transections of fibers which enter or leave hypothalamus laterally.

In a study with 36 female albino Sprague-Dawley rats, parasagittal knife cuts along the lateral border of the hypothalamus which transected most of the fibers that enter or leave the hypothalamus laterally reproduced the full spectrum of effects on food intake seen after lateral hypothalamic lesions. Ss were aphagic and adipsic for weeks or months and remained unresponsive to physiological signals that normally regulate feeding and drinking, even after voluntary ingestive behavior returned. Ss did not respond to insulin or 2-deoxy-D-glucose treatments (which decrease blood sugar or glucose utilization) and showed only a small increase in intake when exposed to low environmental temperatures. Food consumption appeared to be governed primarily by the palatability of the diet. Ss also did not respond to NaCl or polyethylene glycol treatments (which result in cellular dehydration or extracellular hypovolemia) and returned to a state of adipsia when food was removed. (30 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A study of the microstructural evolution during selective laser melting of Ti–6Al–4V

Selective laser melting (SLM) is an additive manufacturing technique in which functional, complex parts can be created directly by selectively melting layers of powder. This process is characterized by highly localized high heat inputs during very short interaction times and will therefore significantly affect the microstructure. In this research, the development of the microstructure of the Ti–6Al–4V alloy processed by SLM and the influence of the scanning parameters and scanning strategy on this microstructure are studied by light optical microscopy. The martensitic phase is present, and due to the occurrence of epitaxial growth, elongated grains emerge. The direction of these grains is directly related to the process parameters. At high heat inputs it was also found that the intermetallic phase Ti₃Al is precipitated during the process.     

Translational regulator RpL10p/Grc5p interacts physically and functionally with Sed1p,a dynamic component of the yeast cell surface

Biogenesis of an active ribosome complement and a dynamic cell surface complement are two major determinants of cellular growth. In yeast, the 60S ribosomal subunit protein RpL10p/Grc5p functions during successive stages in ribosome biogenesis, specifically rRNA processing, nucle(ol)ar preribosomal subunit assembly, nucleo-cytoplasmic transport and cytoplasmic maturation of ribosomes. Here, we report that a two-hybrid screen identified yeast genes SED1, ACS2 and PLB3 as encoding proteins physically interacting with both ribosomal RpL10p/Grc5p and its human homologue hRpL10p/QMp. SED1 encodes a differentially expressed cell wall protein which is proposed to be first transiently secreted to the plasma membrane as a GPI (glycosylated derivative of phosphoinositol)-anchored form and to be then transferred to the glucan layer of the cell wall. Ectopic expression of SED1 rescues both the aberrant growth phenotype and the translation defect of grc5-1(ts) temperature-sensitive cells. Furthermore, we report that Sed1p associates with translating ribosomes suggesting a novel, cytoplasmic role for Sed1p. ACS2 encodes one of the two yeast acetyl-CoA synthases and represents a key enzyme in one of several metabolic routes to produce acetyl-CoA, which in turn is indispensable for lipid biosynthesis. PLB3 encodes a phospholipase, which is active in the breakdown of membrane lipids. Our results support the view that Grc5p/RpL10p links ribosome function to membrane turnover and cell surface biogenesis.