An automated, parallel processing approach to biomolecular sample preparation

Sample preparation for DNA and RNA assays is a prime candidate for laboratory automation. A novel, parallel processing device that performs the three separate liquid-handling functions necessary for such sample preparation-dispensing, pipetting, and pressurizing-is presented. The device comprises an array of fine nozzles connected by fluidic channels to automatically and precisely distribute flow between one source and an array of points. The design principles, as well as the experimental and computational methods used to develop the device, are described. Test results, including accuracy, uniformity, volume range, and timing, are presented. The functionality of the device is demonstrated by performing a solid-phase extraction of DNA with two types of microcolumns.     

The inhibitive effect of extraneous ion addition on stress corrosion cracking of alpha-brass

The addition of ammonium chloride, cobalt sulphate, cesium sulphate and ethylene diamine to a Mattsson solution and the addition of ammonium sulphate to the Pugh solution have been found to produce an inhibitive effect on the stress corrosion cracking of alpha-brass in these solutions. Ammonium chloride and ammonium sulphate additions affect the tarnish formation on the specimen as well as the cracking pattern. Electrochemical polarization studies have also been carried out with these two additives. The observations have been explained in the light of adsorption of these ionic species at selected sites on the metal surface and their possible interference with the cathodic reactions.     

Mass transfer from a carbon dioxide jet to tap water

When a jet of soluble gas is directed on to liquid surface, the kinetic energy of the jet is transferred to the liquid giving rise to circulation, which in turn enhances mass transfer. Consequently, the mass transfer coefficient is related to the characteristics of the jet.     

The effects of silicon on the properties of tantalum

The microalloying of tantalum with silicon has been effectively used on a commercial scale. However, data on effects of microalloying on processibility, microstructure and mechanical properties are not readily available. To develop some data, tantalum powder with various levels of silicon (0–50 ppm) was processed under identical conditions. Sheet samples were then annealed at different temperatures and evaluated via microstructural, chemical and mechanical tests. The recrystallization temperature was found to increase with silicon content, most probably as a result of the presence of an intermetallic (Ta₃Si) phase. The mechanical properties of unrecrystallized tantalum are, as expected, significantly different from those of recrystallized tantalum.     

Collaborative jamming and collaborative defense in cognitive radio networks

In cognitive radio networks, cognitive nodes operate on a common pool of spectrum where they opportunistically access and use parts of the spectrum not being used by others. Though cooperation among nodes is desirable for efficient network operations and performance, there might be some malicious nodes whose objective could be to hinder communications and disrupt network operations. The absence of a central authority or any policy enforcement mechanism makes these kinds of open-access network more vulnerable and susceptible to attacks.In this paper, we analyze a common form of denial-of-service attack, i.e., collaborative jamming. We consider a network in which a group of jammers tries to jam the channels being used by legitimate users who in turn try to evade the jammed channels. First, we compute the distribution of the jamming signal that a node experiences by considering a random deployment of jammers. Then, we propose different jamming and defending schemes that are employed by the jammers and legitimate users, respectively. In particular, we model and analyze the channel availability when the legitimate users randomly choose available channels and the jammers jam different channels randomly. We propose a multi-tier proxy-based cooperative defense strategy to exploit the temporal and spatial diversity for the legitimate secondary users in an infrastructure-based centralized cognitive radio network. Illustrative results on spectrum availability rates show how to improve resiliency in cognitive radio networks in the presence of jammers.     

Cashing in on Caching: An Architecture for Time-Bounded Knowledge-Based Problem Solving

Knowledge-based computing, in general, suffers from an inherent open-endedness that precludes its application in time-bounded domains where an answer must be computed within a stipulated time limit. We examine a two-way improvement of the shortcomings: a knowledge representation scheme that provides easy access to relevant knowledge and thereby reduces search time, and a reasoning scheme that is algorithmic in nature and thus makes computational requirements meaningfully estimable.In this work, we offer a cache-based architecture that is capable of both storing knowledge in different formats (e.g. rules, cases), and invoking an appropriate reasoning scheme to fit the available computing time. The cache helps in retrieving the most relevant pieces of knowledge (not only exact matches) required for solving a given problem. This cache relies on a reasoning tactic, knowledge interpolation, that can generate a solution from two near-matches in an algorithmic way, to generate time-bounded solutions. We illustrate the design of such a cache for solving resource allocation problems in the domain of shortwave radio transmission and evaluate its performance in observing imposed temporal bounds.     

Influential rule search scheme (IRSS) - a new fuzzy pattern classifier

Automatic generation of fuzzy rule base and membership functions from an input-output data set, for reliable construction of an adaptive fuzzy inference system, has become an important area of research interest. We propose a new robust, fast acting adaptive fuzzy pattern classification scheme, named influential rule search scheme (IRSS). In IRSS, rules which are most influential in contributing to the error produced by the adaptive fuzzy system are identified at the end of each epoch and subsequently modified for satisfactory performance. This fuzzy rule base adjustment scheme is accompanied by an output membership function adaptation scheme for fine tuning the fuzzy system architecture. This iterative method has shown a relatively high speed of convergence. Performance of the proposed IRSS is compared with other existing pattern classification schemes by implementing it for Fisher's iris data problem and Wisconsin breast cancer data problems.