Development of high 1-deoxynojirimycin (DNJ) content mulberry tea and use of response surface methodology to optimize tea-making conditions for highest DNJ extraction

Mulberry 1-deoxynojirimycin (DNJ), a potent α-glucosidase inhibitor, suppresses postprandial blood glucose, thereby possibly preventing diabetes mellitus. At present, mulberry dry teas are commercially supplied as functional foods in many countries, but these products may not provide an effective dose (6 mg DNJ/60 kg human wt) due to their low DNJ content (about 100 mg/100 g of dry wt). Therefore, development of tea with higher DNJ content is desirable. To do this, we investigated distribution of DNJ content and α-glucosidase inhibitory activity in 35 Thai mulberry varieties. DNJ content in young leaves varied among mulberry varieties from 30 to 170 mg/100 g of dry leaves. Varieties having highest DNJ content were Kam, Burirum 60 and Burirum 51. Leaf position affected DNJ content: shoots > young leaves > mature leaves. DNJ concentration and α-glucosidase inhibitory activity were highly correlated (r = 0.84), suggesting that α-glucosidase inhibitory activity of mulberry leaves is mainly due to DNJ. Consequently, high DNJ content mulberry tea was produced from shoots of varieties such as Burirum 60, which contains 300 mg/100 g of dry wt. Tea-making conditions were optimized for highest DNJ extraction using response surface methodology. Approximate 95% of total DNJ in high DNJ content dry tea was extracted when temperature was maintained at 98 °C for 400 s; these conditions could be applicable for preparation of commercial products with high DNJ content. One cup (230 ml, a normal serving) of DNJ-enriched mulberry tea contained enough DNJ (6.5 mg) to effectively suppress postprandial blood glucose.     

Heat transfer enhancement and pressure drop characteristics of TiO2–water nanofluid in a double-tube counter flow heat exchanger

This article reports an experimental study on the forced convective heat transfer and flow characteristics of a nanofluid consisting of water and 0.2 vol.% TiO₂ nanoparticles. The heat transfer coefficient and friction factor of the TiO₂–water nanofluid flowing in a horizontal double-tube counter flow heat exchanger under turbulent flow conditions are investigated. The Degussa P25 TiO₂ nanoparticles of about 21 nm diameter are used in the present study. The results show that the convective heat transfer coefficient of nanofluid is slightly higher than that of the base liquid by about 6–11%. The heat transfer coefficient of the nanofluid increases with an increase in the mass flow rate of the hot water and nanofluid, and increases with a decrease in the nanofluid temperature, and the temperature of the heating fluid has no significant effect on the heat transfer coefficient of the nanofluid. It is also seen that the Gnielinski equation failed to predict the heat transfer coefficient of the nanofluid. Finally, the use of the nanofluid has a little penalty in pressure drop.     

Purification and Characterization of Organic Solvent and Detergent Tolerant Lipase from Thermotolerant Bacillus sp. RN2

The aim of this study was to characterize the organic solvent and detergent tolerant properties of recombinant lipase isolated from thermotolerant Bacillus sp. RN2 (Lip-SBRN2). The isolation of the lipase-coding gene was achieved by the use of inverse and direct PCR. The complete DNA sequencing of the gene revealed that the lip-SBRN2 gene contains 576 nucleotides which corresponded to 192 deduced amino acids. The purified enzyme was homogeneous with the estimated molecular mass of 19 kDa as determined by SDS-PAGE and gel filtration. The Lip-SBRN2 was stable in a pH range of 9-11 and temperature range of 45-60 °C. The enzyme was a non metallo-monomeric protein and was active against pNP-caprylate (C8) and pNP-laurate (C12) and coconut oil. The Lip-SBRN2 exhibited a high level of activity in the presence of 108% benzene, 102.4% diethylether and 112% SDS. It is anticipated that the organic solvent and detergent tolerant enzyme secreted by Bacillus sp. RN2 will be applicable as catalysts for reaction in the presence of organic solvents and detergents.     

A model of energy management analysis,case study of a sugar factory in Turkey

This study presents a case study of energy management in a sugar factory in Turkey. The main idea of the study is to analyse energy consumption, the quantity of material production, and figure out a suitable energy efficiency for the case study of a sugar factory subsequently. Firstly, a material production and energy consumption audit were performed for the sugar factory. Secondly, energy efficiency was calculated from the energy data. The SPSS (Statistical Package for the Social Sciences) statistical software was used to ensure the accuracy of the data. The factory’s energy consumption was calculated as 43,590.25 toe (tons of oil equivalent) over the last year. These results were used for CUSUM (Cumulative Sum Deviation Method) graphics. This research poses the consumption of energy, cost of energy and the relationship between energy usage and material production of sugar. The unit of energy cost was 688.22 [$/toe] for the last year. This result showed that the factory decreased the unit of energy by optimisation. The results indicated that the investigated sugar factory should pay attention to the energy management issue in order to comply with the Energy Efficiency of Turkish Law and Directives.     

Characterization of Bi2S3 nanorods and nano-structured flowers prepared by a hydrothermal method

Bismuth sulfide nanorods and nano-structured flowers were synthesized by hydrothermal reaction of bismuth nitrate pentahydrate and thiourea solutions, containing 1 and 2 ml of 65% HNO₃, respectively. By using X-ray diffraction (XRD), selected area electron diffraction (SAED), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM) and high resolution TEM (HRTEM), the products were specified as orthorhombic Bi₂S₃ in the shapes of nanorods and flower-like clusters of nanorods, with the growth of nanorods in the [001] direction. A diffraction pattern was also simulated, and was in good accordance with the SAED pattern obtained from the experiment.     

Transient solid-state production of nanostructured CuS flowers

CuS (hcp) with different morphologies was produced using a transient solid-state reaction by the direct flow of electricity through solids, containing 1:1 molar ratio of Cu:S powders, in a high vacuum system for different lengths of time. X-ray diffraction (XRD), selected area electron diffraction (SAED), and scanning and transmission electron microscopies (SEM and TEM) specified that the products were nanostructured CuS flowers, and nanostructured CuS composing of nanoparticles with different orientations, controlled by the length of time. Raman vibrations were detected at 474.5 cm^− 1, and photoluminescent (PL) emissions at 347.5 nm. Both the XRD and SAED patterns are in accordance with those obtained by the corresponding simulations.     

Experimental investigation of evaporation heat transfer coefficient and pressure drop of R-410A in a multiport mini-channel

The evaporation heat transfer coefficient and pressure drop of R-410A flowing through a horizontal aluminium rectangular multiport mini-channel having 3.48 mm hydraulic diameter are experimentally investigated. The test runs are performed at mass flux ranging between 200 and 400 kg/m² s. The heat fluxes are between 5 and 14.25 kW/m² and the saturation temperatures range between 10 and 30 °C. The pressure drop across the test section is directly measured by a differential pressure transducer. The effects of the imposed wall heat flux, mass flux, vapour quality, and saturation temperature on the evaporation heat transfer and pressure drop are also discussed. The results from the present experiment are compared with those obtained from the existing correlation. New correlations for the evaporation heat transfer coefficient and pressure drop of R-410A flowing through a multiport mini-channel are proposed for practical applications.     

Solid-state synthesis of cubic ZnTe nanocrystals using a microwave plasma

Cubic ZnTe nanocrystals were produced from 1:1 and 1.8:1 molar ratios of Zn:Te by a 900 W microwave plasma. The phase was detected using X-ray diffraction (XRD), which are in accordance with those of the simulations, and selected area electron diffraction (SAED). Raman spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the products were nanocrystals with different orientations, including three longitudinal optical (LO) vibrations at 205, 410 and 620 cm^? 1 and a transverse optical (TO) vibration at 166 cm^? 1. Their green emissions were detected at 562 nm (2.21 eV) using luminescence spectrophotometry.     

Effect of throat diameters of the ejector on the performance of the refrigeration cycle using a two-phase ejector as an expansion device

This paper is a part in a series that reports on the experimental study of the performance of the two-phase ejector expansion refrigeration cycle. In the present study, three two-phase ejectors are used as an expansion device in the refrigeration cycle. The effects of throat diameter of the motive nozzle, on the coefficient of performance, primary mass flow rate of the refrigerant, secondary mass flow rate of the refrigerant, recirculation ratio, average evaporator pressure, compressor pressure ratio, discharge temperature and cooling capacity, which have never before appeared in open literature, are presented. The effects of the heat sink and heat source temperatures on the system performance are also discussed.