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排序方式: 共有298条查询结果,搜索用时 15 毫秒
1.
Till Schümmer Peter Tandler Joerg M. Haake 《Universal Access in the Information Society》2012,11(3):239-258
Meetings are an important form of interaction in business settings. However, meetings are often not held in a way that makes all participants feel engaged. Meeting support systems try to overcome this obstacle by helping meeting participants in the meeting workflow. This paper takes a complementary approach by distinguishing between different perspectives of good meetings: the peopleware perspective, the generic groupware perspective, and the roomware perspective. It is argued that all these perspectives have to be considered simultaneously when heading toward an ideal meeting system design. The paper presents excerpts of a pattern language that empowers users to improve their meeting. Besides this, the pattern language can be considered as a tool for the designer. After showing, how both groups made use of the patterns, the paper finally presents LivingAgendas, a meeting support system that can accompany the meeting participant through the meeting life cycle. 相似文献
2.
König Till Hagen S. P. Virtanen S. Galetz M. C. 《Metallurgical and Materials Transactions A》2022,53(11):4023-4033
Metallurgical and Materials Transactions A - Co-based superalloys have been developed as candidate materials to replace Ni-based superalloys in hot sections of turbine engines, however, their... 相似文献
3.
For a number of programming languages, among them Eiffel, C, Java, and Ruby, Hoare-style logics and dynamic logics have been developed. In these logics, pre- and postconditions are typically formulated using potentially effectful programs. In order to ensure that these pre- and postconditions behave like logical formulae (that is, enjoy some kind of referential transparency), a notion of purity is needed. Here, we introduce a generic framework for reasoning about purity and effects. Effects are modelled abstractly and axiomatically, using Moggi’s idea of encapsulation of effects as monads. We introduce a dynamic logic (from which, as usual, a Hoare logic can be derived) whose logical formulae are pure programs in a strong sense. We formulate a set of proof rules for this logic, and prove it to be complete with respect to a categorical semantics. Using dynamic logic, we then develop a relaxed notion of purity which allows for observationally neutral effects such writing on newly allocated memory. 相似文献
4.
Chung JY Lee SJ Kris Y Braunschweig T Traicoff JL Hewitt SM 《Proteomics. Clinical applications》2008,2(10-11):1539-1547
Proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue offers significant diagnostic utility but is complicated due to the high level of covalently crosslinked proteins arising from formalin fixation. To address these challenges, we developed a reliable protein extraction method for FFPE tissue, based on heat-induced antigen retrieval within a pressure cooker. The protein extraction yield from archival FFPE tissue section is approximately 90% of that recovered from frozen tissue. This method demonstrates preservation of immunoreactivity and recovery of full-length proteins by Western blotting. Additionally, we developed a well-based RP protein array platform utilizing an electrochemiluminescence detection system. Protein samples derived from FFPE tissue by means of laser capture dissection, with as few as 500 shots demonstrate measurable signal differences for different proteins. The lysates coated to the array plate, remain stable over 1?month at room temperature. Theses data suggest that this new protein-profiling platform coupled with the protein extraction method can be used for molecular profiling analysis in FFPE tissue, and contribute to the validation and development of biomarkers in clinical studies. 相似文献
5.
Sheep shearers are known to work in sustained flexed postures and have a high prevalence of low back pain (LBP). As sustained posture and spinal movement asymmetry under substantial loads are known risk factors for back injury our aim was to describe the 3D spinal movement of shearers while working. We hypothesised that thoraco-lumbar and lumbo-sacral movement would be tri-axial, asymmetric, and task specific. Sufficient retro-reflective markers were placed on the trunk of 12 shearers to define thoraco-lumbar and lumbo-sacral 3D motion during three tasks. Thoraco-lumbar movement consistently involved flexion, left lateral flexion, and right rotation. Lumbo-sacral movement consistently involved right lateral flexion in flexion with minimal rotation. Shearers therefore work in sustained spinal flexion where concurrent, asymmetric spinal movements into both lateral flexion and rotation occur. These asymmetric movements combined with repetitive loading may be risk factors leading to the high incidence of LBP in this occupational group. 相似文献
6.
Braunschweig T Kaserer K Chung JY Bilke S Krizman D Knezevic V Hewitt SM 《Proteomics. Clinical applications》2007,1(3):264-271
Thyroid cancer is the most common endocrine neoplasm with multiple histologic subtypes, each associated with different treatments and outcomes. Differentiating benign neoplasms such as follicular adenomas from malignant entities such as follicular carcinomas and papillary carcinoma can be challenging. To define the proteomic profile of different thyroid tumors, we screened an antibody array of 330 features against five thyroid neoplasms: follicular adenoma, follicular carcinoma, papillary carcinoma, anaplastic carcinoma, and medullary carcinoma as well as normal thyroid epithelium. Eight candidate biomarkers; c-erbB-2, Stat5a, Annexin IV, IL-11, RARα, FGF7, Caspase 9, and phospho-c-myc were identified as differentially expressed on the antibody array, and validated with immunohistochemistry on tissue microarrays, with a total of 144 samples of the same variety of thyroid neoplasms. Analysis revealed c-erbB-2, Annexin IV, and Stat5a have potential clinical utility to differentiate follicular adenoma, follicular carcinoma, and papillary carcinoma from each other. By using an antibody array as a discovery platform and a tissue microarray as a first step in validation on a large number of specimens, we have identified new markers that have potential utility in the diagnosis of thyroid neoplasms. 相似文献
7.
Till Böcking Michael James Christopher L. Brown Kevin D. Barrow 《Thin solid films》2006,515(4):1857-1863
Poly(amidoamine) dendrimers were attached to activated undecanoic acid monolayers, covalently linked to smooth silicon surfaces via Si-C bonds. The resulting ultra-thin dendrimer films were characterized by X-ray photoelectron spectroscopy (XPS), X-ray reflectometry (XR) and atomic force microscopy (AFM). XPS results suggested amide bond formation between the dendrimer and the surface carboxylic acid groups. XR yielded thicknesses of 10 Å for the alkyl region of the undecanoic acid monolayer and 12 Å for the dendrimer layer, considerably smaller than the diameter of these spherical macromolecules in solution. This was consistent with AFM images showing collapsed dendrimers on the surface. It was concluded that the deformation arose from a large number of amine groups on the surface of each dendrimer reacting efficiently with the activated surface, whereby the dendrimers can deform to fill voids while spreading over the activated surface to form a homogeneous macromolecular layer. 相似文献
8.
9.
Bernhard Kienesberger Beate Obermüller Georg Singer Barbara Mittl Reingard Grabherr Sigrid Mayrhofer Stefan Heinl Vanessa Stadlbauer Angela Horvath Wolfram Miekisch Patricia Fuchs Ingeborg Klymiuk Holger Till Christoph Castellani 《International journal of molecular sciences》2021,22(12)
We aimed to assess the in vitro antimicrobial activity and the in vivo effect on the murine fecal microbiome and volatile organic compound (VOC) profile of (S)-reutericyclin. The antimicrobial activity of (S)-reutericyclin was tested against Clostridium difficile, Listeria monocytogenes, Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Staphylococcus (S.) epidermidis, Streptococcus agalactiae, Pseudomonas aeruginosa and Propionibacterium acnes. Reutericyclin or water were gavage fed to male BALBc mice for 7 weeks. Thereafter stool samples underwent 16S based microbiome analysis and VOC analysis by gas chromatography mass spectrometry (GC-MS). (S)-reutericyclin inhibited growth of S. epidermidis only. Oral (S)-reutericyclin treatment caused a trend towards reduced alpha diversity. Beta diversity was significantly influenced by reutericyclin. Linear discriminant analysis Effect Size (LEfSe) analysis showed an increase of Streptococcus and Muribaculum as well as a decrease of butyrate producing Ruminoclostridium, Roseburia and Eubacterium in the reutericyclin group. VOC analysis revealed significant increases of pentane and heptane and decreases of 2,3-butanedione and 2-heptanone in reutericyclin animals. The antimicrobial activity of (S)-reutericyclin differs from reports of (R)-reutericyclin with inhibitory effects on a multitude of Gram-positive bacteria reported in the literature. In vivo (S)-reutericyclin treatment led to a microbiome shift towards dysbiosis and distinct alterations of the fecal VOC profile. 相似文献
10.
Özlem Erol Till F. Schäberle Dr. Alexander Schmitz Shwan Rachid Dr. Cristian Gurgui Dr. Mustafa El Omari Friederike Lohr Stefan Kehraus Dr. Jörn Piel Prof. Dr. Rolf Müller Prof. Dr. Gabriele M. König Prof. Dr. 《Chembiochem : a European journal of chemical biology》2010,11(9):1253-1265
Corallopyronin A is a myxobacterial compound with potent antibacterial activity. Feeding experiments with labelled precursors resulted in the deduction of all biosynthetic building blocks for corallopyronin A and revealed an unusual feature of this metabolite: its biosynthesis from two chains, one solely PKS‐derived and the other NRPS/PKS‐derived. The starter molecule is believed to be carbonic acid or its monomethyl ester. The putative corallopyronin A biosynthetic gene cluster is a trans‐AT‐type mixed PKS/NRPS gene cluster, containing a β‐branching cassette. Striking features of this gene cluster are a NRPS‐like adenylation domain that is part of a PKS‐type module and is believed to be responsible for glycine incorporation, as well as split modules with individual domains occurring on different genes. It is suggested that CorB is a trans‐acting ketosynthase and it is proposed that it catalyses the Claisen condensation responsible for the interconnection of the two chains. Additionally, the stereochemistry of corallopyronin A was deduced by a combination of a modified Mosher's method and ozonolysis with subsequent chiral GC analyses. 相似文献