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1.
Jet injection can be used to introduce genes into the cells of differentiated tissues of living animals and organ cultures. When a solution of plasmid DNA is jet injected into a selected tissue or organ, cells lying in or near the path of the jet injection are transfected with the DNA and the introduced gene(s) are expressed. Since there is minimal morbidity from each jet injection, multiple injections can be performed at the same or nearby sites. Both mRNA and protein expression from transfected genes can be quantitated using standard methods. In addition, the technique is an efficient means of DNA immunization. Methodology for using jet injection to transfer plasmid DNA into the cells of skin, fat, mammary gland, and muscle are described. 相似文献
2.
VF Imshenetskaia 《Canadian Metallurgical Quarterly》1992,37(9):23-25
The results of clinical and laboratory studies on the use of augmentin in severe purulent complications after neurosurgical operations are presented. The laboratory studies carried out with the use of an automatic system Cobas Bact (Roch) showed that the numbers of the augmentin resistant strains of Staphylococcus and Enterobacteriaceae among the pathogens were 47 and an average of 64.5%, respectively. Gram-negative bacteria resistant to augmentin were 1.5 to 2 times less frequent than those resistant to amoxycillin. Still, they were much more frequent than those resistant to cefotaxime and ceftriaxone. Clinical efficacy of augmentin was studied in treatment of 39 patients with various affections of the brain such as tumors, trauma, vascular malformations and inflammatory processes. The postoperative complications were represented by meningitis, pneumonia, sepsis and their associations. The use of augmentin in the severe intra- and extracranial complications was favourable in 82.1% of the cases. 相似文献
3.
4.
V. A. Pilipovich V. L. Malevich G. D. Ivlev V. V. Zhidkov 《Journal of Engineering Physics and Thermophysics》1985,48(2):228-233
On the basis of a numerical solution of the Stefan problem, the dynamics of nanosecond laser annealing of silicon is investigated.Translated from Inzhenerno-Fizicheskii Zhurnal, Vol. 48, No. 2, pp. 306–312, February, 1985. 相似文献
5.
This article proposes an approach for investigating the exponential stability of a nonlinear
interval dynamical system with the nonlinearity of a quadratic type on the basis of the Lyapunov’s direct method. It also
constructs an inner estimate of the attraction domain to the origin for the system under consideration. 相似文献
6.
L. P. Kazakova A. A. Gundyrev V. P. Prokof'ev V. P. Ivlev 《Chemistry and Technology of Fuels and Oils》1990,26(5):237-242
Translated from Khimiya i Tekhnologiya Topliv i Masel, No. 5, pp. 17–20, May, 1990. 相似文献
7.
In this paper we consider the asymptotic stability of linear interval time-delay systems on the base of using Lyapunov's direct method and methods of interval analysis. A sufficient condition of asymptotic stability is obtained using the concept of Lyapunov-Krasovsky functional. 相似文献
8.
V. A. Volodin E. I. Gatskevich A. V. Dvurechenskii M. D. Efremov G. D. Ivlev A. I. Nikiforov D. A. Orekhov A. I. Yakimov 《Semiconductors》2003,37(11):1315-1320
The effect of pulsed ruby laser radiation on Ge nanoclusters grown on a (100)-oriented Si substrate is studied. The energy density of radiation corresponds to the melting threshold of the Si surface. Changes in the structure of nanoclusters are analyzed by comparing the experimental Raman spectra to those calculated in terms of Born-von Karman and Vol’kenstein models. It is established that the action of one pulse changes the cluster size and partly relieves the compression. Still greater changes take place in a sample subjected to ten pulses. The Ge nanoclusters transform into clusters of GexSi1?x solid solution, presumably due to the stress-and vacancy-aided diffusion. Laser-induced thermal processes in germanium nanoclusters in silicon are numerically simulated. 相似文献
9.
Novikov H. A. Batalov R. I. Bayazitov R. M. Faizrakhmanov I. A. Lyadov N. M. Shustov V. A. Galkin K. N. Galkin N. G. Chernev I. M. Ivlev G. D. Prokop’ev S. L. Gaiduk P. I. 《Semiconductors》2015,49(6):729-735
Semiconductors - The structural and optical properties of thin Ge films deposited onto semiconducting and insulating substrates and modified by pulsed laser radiation are studied. The films are... 相似文献
10.