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1.
Douwe W. van der Meer Daniel Milazzo Aldo Sanguineti G. Julius Vancso 《Polymer Engineering and Science》2005,45(4):458-468
It is known that friction deposited polytetrafluoroethylene (PTFE) layers are able to nucleate crystallization of thin films of isotactic polypropylene (iPP). In order to investigate the influence of PTFE on the crystallization behavior and morphology of iPP in bulk, PTFE‐particles of two different sizes in various concentrations were melt‐blended with iPP and subsequently processed by injection molding. For one size of particles, high resolution scanning electron microscopy (HR‐SEM) showed the presence of a PTFE scaffold consisting of highly fibrillated PTFE particles. With X‐ray diffraction (WAXD) pole‐figures, it was evidenced that, after melting and recrystallization of the iPP matrix, a strongly oriented crystallization of iPP on this PTFE scaffold takes place (quiescent crystallization conditions). With WAXD it was also shown that under processing conditions, PTFE acts as a nucleating agent for iPP and that PTFE strongly enhances the formation of processing induced morphologies. Impact and tensile performance of the mixtures were measured. Both the strain energy release rate (GI) and the E‐modulus were found to increase upon introducing PTFE in iPP. POLYM. ENG. SCI., 45:458–468, 2005. © 2005 Society of Plastics Engineers. 相似文献
2.
The combination of surfactant modified montmorillonite (MMT) silicate layers, poly(ε-caprolactone) (PCL) and the adopted melt processing procedure results in intercalated nanocomposites in which the silicate layers act as nucleating agents for the crystallization of the PCL matrix and by which in turn the overall crystallization rate increases. At a sufficiently high MMT concentration and degree of supercooling the polymer-swollen silicate layer stacks disturb crystal growth, resulting in a decrease in the overall crystallization rate. Simultaneous, time resolved, synchrotron small and wide angle X-ray scattering experiments reveal that—when the retarding effect is absent at a sufficiently high temperature—the final semicrystalline structures of pure PCL and its nanocomposites are identical. The poorer nucleation in the case of pure PCL, however, results in a time wise smearing of primary and secondary crystallization whereas in the nanocomposites these events are well separated due to a nucleation induced, efficient and rapid primary crystallization. Secondary crystallization involves the insertion of new lamellar crystals in between the already existing ones. 相似文献
3.
Joana Oliveira Jennifer Mahony Laurens Hanemaaijer Thijs R.H.M. Kouwen Douwe van Sinderen 《Journal of dairy science》2018,101(1):96-105
In the current study, we characterized 137 Lactococcus lactis bacteriophages that had been isolated between 1997 and 2012 from whey samples obtained from industrial facilities located in 16 countries. Multiplex PCR grouping of these 137 phage isolates revealed that the majority (61.31%) belonged to the 936 group, with the remainder belonging to the P335 and c2 groups (23.36 and 15.33%, respectively). Restriction profile analysis of phage genomic DNA indicated a high degree of genetic diversity within this phage collection. Furthermore, based on a host-range survey of the phage collection using 113 dairy starter strains, we showed that the c2-group isolates exhibited a broader host range than isolates of the 936 and P335 groups. 相似文献
4.
Carolien Kroeze Rien Aerts Nico van Breemen Douwe van Dam Peter Hofschreuder Marcel Hoosbeek Jeroen de Klein Klaas van der Hoek Hans Kros Harmke van Oene Oene Oenema Albert Tietema Rob van der Veeren Wim de Vries 《Nutrient Cycling in Agroecosystems》2003,66(1):43-69
This study focuses on the uncertainties in the fate of nitrogen (N) in the Netherlands. Nitrogen inputs into the Netherlands in products, by rivers, and by atmospheric deposition, and microbial and industrial fixation of atmospheric N2 amount to about 4450 Gg N y–1. About 60% of this N is transported out of the Netherlands in products. The fate of the remaining 40%, however, is less clear. We discuss uncertainties in losses to the atmosphere (as ammonia or through denitrification), by leaching and runoff, and in N accumulation in biomass and soils. These processes may account for the fate of about 40% of the N in the Netherlands, and for the fate of about 60% of the N in Dutch agricultural soils. Reducing uncertainties in the estimates of these fluxes is necessary for reducing the impact of excess N in the environment. In particular, monitoring the environmental effects of ammonia emissions and nitrate leaching to groundwater and aquatic systems requires an increased understanding of the fate of N. Uncertainties arise because (1) some N fluxes cannot be measured directly and are usually quantified indirectly as the balance in N budgets, (2) direct measurements of N fluxes have inevitable inaccuracies, (3) lack of experimental data and other information (e.g. statistics) needed for upscaling, (4) large spatial and temporal variability of fluxes, and (5) poor understanding of the processes involved. These uncertainties can be reduced by additional experimental studies and by further development of process-based models and N budget studies. We prioritize these future research needs according to a range of different criteria. 相似文献
5.
Compression of cylindrical samples was found to be a sensitive method in detecting differences in textural attributes in stick
margarines. Constant speed penetration by a probe was the second most sensitive method, while American Oil Chemists’ Society
(AOCS) cone penetrometer was the least sensitive method. Canola margarines were significantly harder than soybean margarines.
Solid fat content in the product is related to its texture only to a certain extent. The nature of the crystal network is
also of importance. To estimate the solids in the final product by pulsed nuclear magnetic resonance it is best to use the
AOCS cooling method with a tempering step at 25°C. The International Union of Pure and Applied Chemistry (IUPAC) cooling method
results in much higher values. 相似文献
6.
Influence of shear flow on the preparation of polymer layered silicate nanocomposites 总被引:1,自引:0,他引:1
In this paper the influence of melt-processing on the final polymer/layered silicate nanocomposite morphology is discussed. In particular the role of shear forces on the transformation of the original large clay agglomerates is of interest. Several polymer nanocomposites were prepared by melt-extrusion, involving polycaprolactone, poly(ethylene oxide), polyamide-12 or polyamide-6 as the matrix polymer. The nanocomposite morphology was characterised by X-ray diffraction and transmission electron microscopy and the clay tactoid morphology with polarised optical microscopy and scanning electron microscopy. The development of the tactoid and nanocomposite morphology during melt-mixing under shear was studied time-resolved by optical microscopy in conjunction with a rheometer and synchrotron X-ray scattering together with a Couette type flow cell. The shear forces in the melt-preparation of polymer layered mineral nanocomposites facilitate the break-up of large-sized agglomerates, whereas the extent of further exfoliation of the mineral layers is determined by the compatibility between the polymer matrix and the mineral layers rather than by shear forces. 相似文献
7.
Deirdre P. Lowe Helge M. Ulmer Douwe Van Sinderen Elke K. Arendt 《Journal of the Institute of Brewing》2004,110(2):133-140
In this study four strains of lactic acid bacteria (LAB) were chosen to bioacidify a mash containing 50% barley and 50% malt. The strains were isolated from malted and unmalted barley and assayed for extracellular enzymatic activities (proteases, amylases, β‐glucanases). The biologically acidified mash was compared to a chemically acidified mash, 100% malt mash un‐acidified and 50% malt and 50% barley mash unacidified. Characteristics such as pH, extract, colour, viscosity, total soluble nitrogen (TSN), free amino nitrogen (FAN), apparent fermentability, β‐glucan and lautering performance of the resultant worts were determined. A model lautering system replicating one used in a brewery was designed and built in University College Cork (UCC) to measure the lautering performance of the bioacidified mashes. The new system was compared to the filtration method used in EBC method 4.5.1. Overall the addition of LAB to bioacidify a mash of 50% barley and 50% malt resulted in faster filtration times, which correlated with decreased β‐glucan levels. Proteolytic LAB had a positive influence on the quality of wort and resulted in increased FAN levels. Lighter colour worts were observed along with increased extract levels. 相似文献
8.
Rosanne Hertzberger Ali May Gertjan Kramer Isabelle van Vondelen Douwe Molenaar Remco Kort 《International journal of molecular sciences》2022,23(10)
Glycogen in the female lower reproductive tract is a major carbon source for colonization and acidification by common vaginal Lactobacillus species, such as Lactobacillus crispatus. Previously, we identified the amylopullulanase encoding gene pulA of Lactobacillus crispatus to correlate with the ability to autonomously utilize glycogen for growth. Here, we further characterize genetic variation and differential regulation of pulA affecting the presence of its gene product on the outer surface layer. We show that alpha-glucan degrading activity dissipates when Lactobacillus crispatus is grown on glucose, maltose and maltotriose, in agreement with carbon catabolite repression elements flanking the pulA gene. Proteome analysis of the S-layer confirmed that the amylopullulanase protein is highly abundant in an S-layer enriched fraction, but not in a strain with a defective amylopullulanase variant or in an amylopullulanase-sufficient strain grown on glucose. In addition, we provide evidence that Lactobacillus crispatus pulA mutants are relevant in vivo, as they are commonly observed in metagenome datasets of human vaginal microbial communities. Analysis of the largest publicly available dataset of 1507 human vaginal metagenomes indicates that among the 270 samples that contain a Lactobacillus crispatus pulA gene, 62 samples (23%) had a defective variant of this gene. Taken together, these results demonstrate that both environmental, as well as genetic factors explain the variation of Lactobacillus crispatus alpha-glucosidases in the vaginal environment. 相似文献
9.
PW Kunst A Vonk Noordegraaf OS Hoekstra PE Postmus PM de Vries 《Canadian Metallurgical Quarterly》1998,19(4):481-490
Cytokine gene transfer into tumor cells has been shown to mediate tumor regression and antimetastatic effects in several animal models via immunomodulation. Therefore, clinical protocols have been developed to treat cancer patients with cytokine gene-modified tumor cells. We inserted the genes coding for the p35 and p40 chain of interleukin-12 (IL-12) in two independent eukaryotic expression vectors and transduced melanoma cells of 15 different primary tumor cultures with both plasmids by a ballistic gene transfer approach. Secreted IL-12 demonstrated strong bioactivity by inducing interferon-gamma release from peripheral blood lymphocytes upon coculture with cell culture supernatants after IL-12 gene transfer which could at least partly be blocked by IL-12-specific antisera. Further enrichment of transduced tumor cells by magnetic separation directly after gene transfer increased cytokine secretion from a mean of 119 pg in the unsorted to 507 pg IL-12 (24 h/10(8) cells) in the magnetically enriched cell fraction. Irradiation of these cells led to a further elevation of secreted IL-12 (mean 987 pg). Elevated IL-12 levels were detected over 7 days after irradiation in vitro. In a subsequent first clinical phase I study six patients with metastatic melanoma were vaccinated with autologous, interleukin-12 gene-modified tumor cells. Melanoma cells were expanded in vitro from surgically removed metastases, transduced by ballistic gene transfer, irradiated and were then injected subcutaneously (s.c.) at weekly intervals. Clinically, there was no major toxicity except for mild fever. All patients completed more than four s.c. vaccinations over 6 weeks and were eligible for immunological evaluation. Post-vaccination, peripheral mononuclear cells were found to contain an increased number of tumor-reactive proliferative as well as cytolytic cells as determined by a limiting dilution analysis in two patients. Two patients developed DTH reactivity against autologous melanoma cells and one had a minor clinical response. Biopsies taken from that patient's metastases revealed a heavy infiltration of CD4+ and CD8+ T lymphocytes. In conclusion, vaccination induced immunological changes even in a group of advanced, terminally ill patients. These changes can be interpreted as an increased antitumor immune response. 相似文献
10.
EF Smit E Fokkema B Biesma HJ Groen W Snoek PE Postmus 《Canadian Metallurgical Quarterly》1998,77(2):347-351
Deep vein thrombosis (DVT) and pulmonary embolism (PE) are major complications associated with total knee arthroplasty. The American College of Chest Physicians recommends twice-daily, fixed-dose low-molecular-weight heparin (LMWH) as routine prophylaxis in this patient population. This study represents a cost analysis of ardeparin and enoxaparin, the two LMWHs currently available for this indication in the United States. Costs for treating DVT, PE, and major bleeding episodes were derived from values reported in the literature. Both ardeparin and enoxaparin were found to produce significant cost savings when used routinely as DVT prophylaxis after knee replacement surgery compared with no prophylaxis. Based on the currently available data, enoxaparin 40 mg once daily appears to be the least costly LMWH for routine pharmacoprophylaxis of DVT in patients undergoing knee replacement surgery. 相似文献