Influences of copolymers (Copovidone,Eudragit RL PO and Kollicoat MAE 30 DP) on stability and bioactivity of spray-dried and freeze-dried lysozyme

Protein stability is the most crucial factor in protein pharmaceutical preparations. Various techniques were applied for producing stable protein formulations such as spray-drying and freeze-drying. However, heating and freezing stresses are disadvantages for proteins using these methods, respectively. Accordingly, excipients have been used to preserve therapeutic effects of proteins during processing and for long period of time. Therefore, influences of Copovidone, Eudragit^® RL-PO and Kollicoat^® MAE-30 DP (as excipients) on stability and integrity of lysozyme (as a model protein) in spray-dried and freeze-dried forms were investigated. Protein formulations in both dried forms were prepared without and with the addition of mentioned excipients at different concentrations. Protein formulations were characterized for yield determination, morphology using scanning electron microscopic (SEM), thermal analysis by Differential Scanning Calorimetry (DSC), secondary structure stability using Fourier transform infrared (FT-IR) spectroscopy and biological activity. All protein formulations were subjected to a stability study as solid protein formulations for 3 weeks at 24?°C/76% relative humidity and aqueous protein samples were stored at 50?°C for 30?min in a water bath. Results showed that Copovidone successfully preserved integrity and biological activity of lysozyme before and after storage in both spray-dried and freeze-dried forms with more advantage for using higher concentration of the same excipient. Smooth spheres of spray-dried lysozyme formulations with Copovidone were smaller than spray-dried lysozyme without and with Kollicoat^® MAE-30 DP, which affected %yield produced. Copovidone has demonstrated valuable protection ability for lysozyme.     

The addition of paclitaxel to continuous infusion 5-fluorouracil is an active regimen for metastatic breast cancer

We investigated tissue staining for Bcl-2 and Bax proteins, which regulate apoptosis, as indicators of chemotherapeutic effect in patients with gastric cancer. In 23 patients with gastric carcinoma biopsy specimens were obtained endoscopically prior to chemotherapy and stained immunohistochemically with anti-Bcl-2 and anti-Bax antibodies. Patients then were treated with continuous infusion of 5-FU and cisplatin. No correlation was seen between chemotherapeutic effect and Bcl-2 or Bax alone. However, among the Bax-positive cases, the patients with Bcl-2-positive tumors were significantly more chemoresistant (p = 0.036) and had worse prognoses (p = 0.008) than Bcl-2-negative cases. Therefore, immunohistochemical staining for Bcl-2 protein may predict chemotherapeutic efficacy or guide specific therapeutic choices in treating Bax-positive tumors.     

Enhancement of Infrared Images Using Super Resolution Techniques Based on Big Data Processing

This paper presents a super-resolution (SR) technique for enhancement of infrared (IR) images. The suggested technique relies on the image acquisition model, which benefits from the sparse representations of low-resolution (LR) and high-resolution (HR) patches of the IR images. It uses bicubic interpolation and minimum mean square error (MMSE) estimation in the prediction of the HR image with a scheme that can be interpreted as a feed-forward neural network. The suggested algorithm to overcome the problem of having only LR images due to hardware limitations is represented with a big data processing model. The performance of the suggested technique is compared with that of the standard regularized image interpolation technique as well as an adaptive block-by-block least-squares (LS) interpolation technique from the peak signal-to-noise ratio (PSNR) perspective. Numerical results reveal the superiority of the proposed SR technique.

     

A Structural Damage Neural Network Monitoring System

Abstract: Traditional methods for structural monitoring and damage assessment have been implemented largely through visual inspection and on-site tests. A system for automating this process should be able to record the various signatures of the structure to be monitored and issue a warning signal if there is a damage-related change in those signatures. In this paper, a general system for structural damage monitoring is proposed based on observations of other researchers and the results obtained from a case study of a physical and analytical model of a five-story steel frame. The proposed diagnostic system utilizes neural networks for identifying the damage associated with changes in structural signatures. The system is independent of the type of signatures used for monitoring. Two sets of neural networks were developed. The first set was trained with the results of a series of shaking-table experiments, while the second set was trained with the output produced from a finite-element model of the same test structure. The results show that the proposed system provides a suitable framework for automatic structural monitoring.     

A randomized trial of recombinant human interleukin-11 following autologous bone marrow transplantation with peripheral blood progenitor cell support in patients with breast cancer

Complications resulting from advanced atherosclerosis are the most common indication for vascular reconstructive surgery. Atherosclerosis is a systemic disease affecting the entire arterial tree, but lesions involving the coronary, extracranial cerebral, and lower extremity circulations have the most clinical significance for surgeons. The pathogenesis of atherosclerosis involves a complex series of events, similar to a chronic inflammatory process, with the formation of atherosclerotic plaque as the end result. Injury to the endothelial cell of the artery, resulting in endothelial cell dysfunction, is the first step in the process. Activated endothelial cells attract leukocytes and vascular smooth muscle cells (VSMC), which accumulate and proliferate in the arterial wall. These cellular components produce an excessive amount of connective tissue matrix. The ultimate end point is the formation of a mature fibrous plaque. Symptoms occur when advanced lesions are complicated by plaque rupture, hemorrhage into the plaque, emboli, or thrombosis. A thorough understanding of the pathogenesis of atherosclerosis is essential for the development of strategies for the prevention of the disease, and for the development of new and effective treatments.