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Iosif Vranakis Anastasia Papadioti Yannis Tselentis Anna Psaroulaki Georgios Tsiotis 《Proteomics. Clinical applications》2013,7(1-2):193-204
Coxiella burnetii, the causative agent of Q fever, is an intracellular bacterium and a potential weapon for bioterrorism. The widespread throughout the world, zoonosis is manifested clinically as a self-limited febrile illness, as pneumonia (acute Q fever) or as a chronic illness with endocarditis being its major complication. The recent Netherlands Q fever outbreak has driven the bacterium from a relatively cryptic, underappreciated, “niche” microorganism on the sideline of bacteriology, to one of possibly great impact on public health. Advances in the study of this microorganism proceeded slowly, primarily due to the, until recently, obligatory intracellular nature of the pathogen that in its virulent phase I must be manipulated under biosafety level-3 conditions. Proteomic studies, in particular, have generated a vast amount of information concerning several aspects of the bacterium such as virulence factors, detection/diagnostic and immunogenic biomarkers, inter-/intraspecies variation, resistance to antibiotics, and secreted effector proteins with significant clinical impact. The phenomenon observed following the genomics era, that of generation and accumulation of huge amount of data that ultimately end up unexploited on several databases, begins to emerge in the proteomics field as well. This review will focus on the advances in the field of C. burnetii proteomics through MS, attempting in parallel to utilize some of the proteomics findings by suggesting future directions for the improvement of Q fever diagnosis and therapy. 相似文献
3.
In the present study, we propose a regression-based scheme for the direct estimation of the height of unknown speakers from
their speech. In this scheme every speech input is decomposed via the openSMILE audio parameterization to a single feature vector that is fed to a regression model, which provides a direct estimation of
the persons’ height. The focus in this study is on the evaluation of the appropriateness of several linear and non-linear
regression algorithms on the task of automatic height estimation from speech. The performance of the proposed scheme is evaluated
on the TIMIT database, and the experimental results show an accuracy of 0.053 meters, in terms of mean absolute error, for
the best performing Bagging regression algorithm. This accuracy corresponds to an averaged relative error of approximately 3%.
We deem that the direct estimation of the height of unknown people from speech provides an important additional feature for
improving the performance of various surveillance, profiling and access authorization applications. 相似文献
4.
Ioannis Basdekis Iosif Klironomos Ioannis Metaxas Constantine Stephanidis 《Universal Access in the Information Society》2010,9(2):185-190
This communication reports on the results of a web accessibility audit of public Web sites in Greece. The audit was conducted
in 2008 by the Human Computer Interaction Laboratory of the Institute of Computer Science of FORTH, in its capacity as the
Greek National Contact Centre of the European Design for All e-Accessibility Network. In an earlier e-Accessibility study
in 2004, that evaluated approximately 250 public and commercial Web sites in Greece, 73% of the sample failed to meet the
most basic requirements for web accessibility (). Four years later, in the context of a re-audit, a similar sample was examined for compliance with the same web accessibility
standard (WCAG 1.0), as set by the Web Accessibility Initiative of the World Wide Web Consortium. The accessibility checks
were conducted during the period from March to September 2008. A comparison of the results indicates that although new web
development technologies may have been employed, instead of improving the overall status of e-accessibility, this has resulted
in a serious deterioration in overall accessibility levels. Such findings—85% of sites failed to comply with Level A—suggest
that Greek Web sites are likely to present even more significant access barriers to people with disability than in the past.
As Web Accessibility is poorly understood and, until today, new platforms have failed to deliver products that conform to
WCAG, the need emerges for a concrete legislative framework to set accessibility specifications for all public Web sites in
Greece. 相似文献
5.
降低焦比是降低铁水生产成本的最有效方法.按测算,高炉最低焦比可降至180~200kg/t.要达到这样的焦比,可以采用两种方法:在喷氧量为210~280kg/t情况下,喷煤量应达到300~400kg/t;喷吹脱CO2后经预热的高炉煤气和纯氧.钢铁联合企业煤气平衡的波动和高喷煤比时高炉回旋区的气化问题是这两种技术的主要制约因素.高炉喷吹煤的气化产物可以有效解决上述问题.该技术与传统喷煤工艺相比,能够提高喷吹比、降低焦比;可使用低级煤;不用把煤磨成细粉;在煤气化过程中可以进行脱硫处理;煤灰可以在该过程中去除掉.开发了一种安装在高炉风口处的煤的特殊气化装置,在乌克兰扎波罗热(Zaporozgstal)钢铁公司的高炉上进行了工业试验,计划喷吹305kg/t煤的气化物和105kg/t的氧气,预计可以将焦比从565kg/t降至305kg/t(如果炉料组成较好,焦比可进一步降至180~200kg/t),高炉利用系数提高50%,铁水生产成本降低10%,向大气放散的气体量将随焦比的下降而成比例地减少.该技术投资比传统喷煤系统低得多,投资回收期在一年以内. 相似文献
6.
Ganty Pierre Iosif Radu Konečný Filip 《International Journal on Software Tools for Technology Transfer (STTT)》2017,19(5):565-584
International Journal on Software Tools for Technology Transfer - We show how to underapproximate the procedure summaries of recursive programs over the integers using off-the-shelf analyzers for... 相似文献
7.
Effect of vitamin E supplementation on neutrophil function, milk composition and plasmin activity in dairy cows in a commercial herd 总被引:1,自引:0,他引:1
Fifty-six Holstein dairy cows from a commercial dairy herd in the Northern part of Greece were used to determine the effect of vitamin E supplementation on immune parameters, milk composition and milk quality. Cows were assigned to one of two experimental groups: control (no vitamin E supplementation) and vitamin E supplementation. Supplementation of vitamin E started 4 weeks prior to and continued up to 12 weeks after parturition. Supplementation included daily oral administration of vitamin E at 3000 i.u./cow prepartum and was reduced to 1000 i.u./cow post partum. Blood samples were collected weekly for 8 weeks starting 4 weeks before parturition, neutrophils were isolated and the following parameters were determined in neutrophils activated by phorbol myristate acetate: total cell-associated and membrane-bound urokinase plasminogen activator (u-PA) activity and superoxide production. Milk samples were collected weekly and fat, protein, lactose, somatic cell count (SCC), plasmin and plasminogen-derived activity were determined. Activated neutrophils isolated from cows that received supplemental vitamin E had higher (P<0.01) total and membrane-bound u-PA activities during the first 3 weeks after parturition and higher (P<0.01) superoxide production during week 1 prepartum and week 1 post partum compared with the corresponding values of activated neutrophils isolated from control cows. Vitamin E supplementation had no effect (P=0.28) on plasminogen-derived activity in milk. Milk obtained from cows that received supplemental vitamin E had SCC lower by 25% (P<0.05) and plasmin lower by 30% (P<0.01) than corresponding values in milk obtained from control cows. The reduction in plasmin as a result of vitamin E supplementation is very beneficial to the dairy industry because plasmin reduces the cheese-yielding capacity of milk, affects the coagulating properties of milk and its overall ability to withstand processing during cheesemaking. In conclusion, vitamin E supplementation had positive effects on the function of bovine neutrophils and milk quality in a commercial dairy herd. 相似文献
8.
Laser systems have been developed to image underwater objects. However, the performance of these systems can be severely degraded in turbid water. We have developed a technique using modulated light to improve underwater detection and imaging. A program, Modulated Vision System (MVS), which is based on a new theoretical approach, has been developed to predict modulated laser imaging performance. Experiments have been conducted in a controlled laboratory environment to test the accuracy of the theory as a function of system and environmental parameters. Results show a strong correlation between experiment and theory and indicate that the MVS program can be used to predict future system performance. 相似文献
9.
Alexandros Tsilfidis Iosif Mporas John Mourjopoulos Nikos Fakotakis 《Computer Speech and Language》2013,27(1):380-395
The performance of recent dereverberation methods for reverberant speech preprocessing prior to Automatic Speech Recognition (ASR) is compared for an extensive range of room and source-receiver configurations. It is shown that room acoustic parameters such as the clarity (C50) and the definition (D50) correlate well with the ASR results. When available, such room acoustic parameters can provide insight into reverberant speech ASR performance and potential improvement via dereverberation preprocessing. It is also shown that the application of a recent dereverberation method based on perceptual modelling can be used in the above context and achieve significant Phone Recognition (PR) improvement, especially under highly reverberant conditions. 相似文献
10.
A new technique has been found that uses in-phase and quadrature phase (I/Q) demodulation to optimize the images produced with an amplitude-modulated laser imaging system. An I/Q demodulator was used to collect the I/Q components of the received modulation envelope. It was discovered that by adjusting the local oscillator phase and the modulation frequency, the backscatter and target signals can be analyzed separately via the I/Q components. This new approach enhances image contrast beyond what was achieved with a previous design that processed only the composite magnitude information. 相似文献