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1.
Synthetic peptide samples may contain counter-ions such as acetate or trifluoroacetate as a result of their method of preparation. Furthermore, because acetic acid (HOAc) and trifluoroacetic acid (TFA) are frequently used reagents in peptide synthesis, these acids may be found in synthetic peptide samples as impurities. This paper describes a method validation to determine HOAc and TFA in synthetic peptide samples by capillary electrophoresis (CE) using an internal standard (I.S.) with indirect UV detection. Typical analytical parameters such as precision, linearity, accuracy, specificity, limit of detection and ruggedness were evaluated during the validation. In addition, the contents of HOAc and TFA in two synthetic opioid peptide samples, TIPP[psi] and Orphanin FQ, were determined using the validated method. A unique feature of the method is that it offers determination of both acids in a single assay using a common I.S. The method is very efficient because of relatively short electrophoretic migration times (typically 2 to 8 min) for the acids investigated. This paper also discusses the factors that affect precision in a CE assay. 相似文献
2.
Geoffrey M. Rowe Jay N. Meegoda Andris Jumikis Mark J. Sharrock Nishantha Bandara Chamil H. Hettiarachchi 《Canadian Metallurgical Quarterly》2004,130(6):924-934
Abstract: This paper describes the research funded by the New Jersey Department of Transportation to develop an automated technology to monitor segregation during construction of hot-mix asphalt concrete pavements. A Laser-based system was used to measure surface texture and to detect segregation. Two segregated test sections and a control test section were tested to evaluate the applicability of Laser texture method to detect and quantify segregation. Laser texture data were gathered from all three sections. Ratios of texture in segregated areas to that in nonsegregated areas were set as the basis for detection of different levels of segregation. By combining the level of segregation and extent of segregation, an AREA index was developed to determine the acceptability of a pavement section. Based on AREA index, pay adjustment factors were proposed to reduce the payment to account for loss of pavement life due to segregation. Further remedial actions were proposed to correct segregated pavement sections with acceptable AREA index. Based on the above concepts, Windows-based computer program NJTxtr was developed to detect and quantify segregation. This computer program uses the Laser-equipment-collected pavement texture data and determines whether the pavement section is acceptable or unacceptable based on the level of segregation within a pavement section, and provides bonus or penalties to the contractor. The paper describes a novel technology using laser and associated software for construction quality control of asphalt concrete pavements. The proposed methodology was applied to detect segregation in an interstate highway section in New Jersey, and this section was repaved based on visual observation and recommendation from this study. 相似文献
3.
A finite element formulation including the effects of shear deformation and cylindrically orthotropic material properties is described for studying the post-buckling behaviour of annular plates. Numerical results for the buckling load parameter and ratios of nonlinear load parameter to buckling load parameter for various values of orthotropic properties, thicknesses and radii ratios of the plates are presented. 相似文献
4.
The corrosion morphologies of four important high nickel alloys, Hastelloy B, Monel 400, Hastelloy C and Inconel 611 in a neutral (pH = 8.3) chloride/bicarbonate medium polarized potentiostatically until an anodic current density of 0.30 mA cm−2 was reached have been studied.It has been shown that Hastelloy B undergoes shallow pitting; Monel 400 produces a porous film with dissolution sites followed by pits covered with corrosion products; Hastelloy C undergoes intergranular corrosion and Inconel 611 undergoes catastrophic pitting. The most superior alloy of these is Hastelloy C. 相似文献
5.
A finite element formulation for the large amplitude free oscillations of beams and orthotropic circular plates is presented in this paper. The present formulation does not need the knowledge of longitudinal/inplane forces developed due to large displacements and thus avoids the use of corresponding geometric stiffness matrices, which were used in earlier finite element formulations. The convergence of the results obtained by using the present formulation is very good. Comparison of the present results with the earlier work wherever possible confirms the reliability and effectiveness of the present finite element formulation. 相似文献
6.
Thermal post-buckling behaviour of tapered columns is investigated through a finite element analysis. Columns of rectangular cross sections with breadth taper (depth being constant), depth taper (breadth being constant) and columns of circular cross sections with diameter taper are considered under simply—supported and clamped boundary conditions. Results for various cases are presented in the form of linear thermal buckling load and empirical formulae for the ratios of nonlinear thermal load to linear thermal buckling load. 相似文献
7.
Large amplitude axisymmetric vibrations of annular plates with the inner and outer edges elastically restrained against rotation, are considered in this paper. A finite element formulation is used to obtain the effect of elastical restraints on the nonlinear to linear period ratios of annular plates of different radii ratios. 相似文献
8.
Baiquan Liu Manoj Sharma Junhong Yu Sushant Shendre Chathuranga Hettiarachchi Ashma Sharma Aydan Yeltik Lin Wang Handong Sun Cuong Dang Hilmi Volkan Demir 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(38)
Copper‐doped colloidal quantum wells (Cu‐CQWs) are considered a new class of optoelectronic materials. To date, the electroluminescence (EL) property of Cu‐CQWs has not been revealed. Additionally, it is desirable to achieve ultrapure green, tunable dual‐emission and white light to satisfy the various requirement of display and lighting applications. Herein, light‐emitting diodes (LEDs) based on colloidal Cu‐CQWs are demonstrated. For the 0% Cu‐doped concentration, the LED exhibits Commission Internationale de L'Eclairage 1931 coordinates of (0.103, 0.797) with a narrow EL full‐wavelength at half‐maximum of 12 nm. For the 0.5% Cu‐doped concentration, a dual‐emission LED is realized. Remarkably, the dual emission can be tuned by manipulating the device engineering. Furthermore, at a high doping concentration of 2.4%, a white LED based on CQWs is developed. With the management of doping concentrations, the color tuning (green, dual‐emission to white) is shown. The findings not only show that LEDs with CQWs can exhibit polychromatic emission but also unlock a new direction to develop LEDs by exploiting 2D impurity‐doped CQWs that can be further extended to the application of other impurities (e.g., Mn, Ag). 相似文献
9.
H.D. Madhawa Hettiarachchi Mihajlo Golubovic William M. Worek W.J. Minkowycz 《International Journal of Heat and Mass Transfer》2008,51(21-22):5088-5096
Three-dimensional laminar slip-flow and heat transfer in rectangular microchannels having constant temperature walls are studied numerically using the finite-volume method for thermally and simultaneously developing flows. The Navier–Stokes and energy equations are solved with velocity slip and temperature jump at the wall. A modified convection–diffusion coefficient at the wall–fluid interface is defined to incorporate the temperature-jump boundary condition. Validity of the numerical simulation procedure is established and the effect of rarefaction on hydrodynamicaly developing flow field, pressure gradient and entrance length is analyzed. A correlation for the fully developed friction factor is presented as a function of Knudsen number (Kn) and aspect ratio (α). The influence of rarefaction on the Nusselt (Nu) number is investigated for thermally and simultaneously developing flows. The effect of velocity slip is found to increase the Nu number, while the temperature-jump tends to decrease it, and the combined effect could result in an increase or a decrease in the Nu number. In the fully developed region, there could be high as 15% increase or low as 50% decrease in Nu number is plausible for the range of parameters considered in this work. 相似文献
10.
Hydraulic Conductivity of MSW in Landfills 总被引:1,自引:0,他引:1
Krishna R. Reddy Hiroshan Hettiarachchi Naveen Parakalla Janardhanan Gangathulasi Jean Bogner Thomas Lagier 《Canadian Metallurgical Quarterly》2009,135(8):677-683
This paper presents a laboratory investigation of hydraulic conductivity of municipal solid waste (MSW) in landfills and provides a comparative assessment of measured hydraulic conductivity values with those reported in the literature based on laboratory and field studies. A series of laboratory tests was conducted using shredded fresh and landfilled MSW from the Orchard Hills landfill (Illinois, United States) using two different small-scale and large-scale rigid-wall permeameters and a small-scale triaxial permeameter. Fresh waste was collected from the working phase, while the landfilled waste was exhumed from a borehole in a landfill cell subjected to leachate recirculation for approximately 1.5 years. The hydraulic conductivity tests conducted on fresh MSW using small-scale rigid-wall permeameter resulted in a range of hydraulic conductivity 2.8×10?3–11.8×10?3?cm/s with dry unit weight varied in a narrow range between 3.9–5.1?kN/m3. The landfilled MSW tested using the same permeameter produced results between 0.6×10?3–3.0×10?3?cm/s for 4.5–5.5?kN/m3 dry unit weights. The hydraulic conductivity obtained from large-scale rigid-wall permeameter tests decreased with the increase in normal stress for both fresh and landfilled waste. The hydraulic conductivity for fresh MSW ranged from 0.2 cm/s for 4.1?kN/m3 dry unit weight (under zero vertical stress) and then decreased to 4.9×10?5?cm/s for 13.3?kN/m3 dry unit weight (under the maximum applied normal stress of 276 kPa). The hydraulic conductivity of the landfilled MSW decreased from 0.2 cm/s to 7.8×10?5?cm/s when the dry unit weight increased from 3.2 to 9.6?kN/m3. The results clearly demonstrated that the hydraulic conductivity of MSW can be significantly influenced by vertical stress and it is mainly attributed to the increase in density leading to low void ratio. In small-scale triaxial permeameter, when the confining pressure was increased from 69 to 276 kPa the hydraulic conductivity decreased from approximately 10?4?to?10?6?cm/s, which is much lower than those determined from rigid-wall permeameter tests. The published field MSW hydraulic conductivities are found to be higher than the laboratory results. Landfilled MSW possesses lower hydraulic conductivity than fresh MSW due to increased finer particles resulting from degradation. The decreasing hydraulic conductivity with increasing dry unit weight is expressed by an exponential decay function. 相似文献