Preparation of glucose oxidase immobilized in different carriers using radiation polymerization

Glucose oxidase (EC 1.1.3.4) was immobilized on different polymeric materials using different immobilization techniques (entrapping by γ‐irradiation, and covalent binding using epichlorohydrin). Studies were carried out to increase the thermal stability of glucose oxidase (GOD) for different applications. The activity and stability of the resulting biopolymers have been compared with those of free GOD. The effect of different polyvinyl alcohol/polyacrylamide (PVA/PAAm) compositions of the copolymer carrier on the enzymatic activity of the immobilized GOD was studied. The maximum enzymatic activity was obtained with the composition ratio of PVA/PAAm of 60:40. The behaviour of the free and immobilized enzyme was analysed as a function of pH. A broadening in the pH profile (5.5–8) was observed for immobilized preparations. The activity and stability of the resulting biopolymers produced by immobilization of GOD onto different carriers have been compared, in both aqueous and organic media, with those of the free GOD. The enzyme's tolerance toward both heat and organic solvent was enhanced by immobilization onto polymers. The addition of different concentrations of organic solvents (10–50%, v/v) to the enzyme at higher temperature (60 °C) was found to stabilize the enzyme molecule. The strongest stabilizing effect on the enzymatic activity was achieved at a concentration of 10%. Copyright © 2005 Society of Chemical Industry     

Plasma and erythrocyte alpha-tocopherol and plasma retinol concentrations in term infants fed formula enriched with long-chain polyunsaturated fatty acids

OBJECTIVE: To assess the effect of long-chain polyunsaturated fatty acids (LCPUFA)- and vitamin E-supplemented formula feeding on erythrocyte and plasma alpha-tocopherol (VE), and plasma retinol (VA) concentrations in neonates and to compare these values with those found in infants feeding on infant formula without LCPUFA or breast milk SETTING: University Hospital of Granada, Spain. SUBJECTS: 49 full-term infants. DESIGN AND INTERVENTION: Subjects who chose not to breast feed were fed either (i) unsupplemented infant formula (F) or (ii) infant formula supplemented with LCPUFA and vitamin E (FL). Alpha-tocopherol and retinol were measured at 7 days, 1 month and 3 months. RESULTS: Plasma and erythrocyte VE concentrations and plasma VE/total lipids ratio increased significantly in all groups at 1 month of life (P < 0.05), but did not change significantly between 1 month and 3 months in any group (P > 0.05). Erythrocyte VE and VA retinol concentrations were higher in infants fed an infant formula than in breast milk-fed infants at 1 month of life (P < 0.05). Finally, there were no significant differences in plasma or erythrocyte VE levels, plasma VA or plasma VE/total lipid ratio between any groups at 3 months of life (P > 0.05). CONCLUSION: Infants fed on LCPUFA- and vitamin E-supplemented infant formula for 3 months have similar vitamin E and A status to infants fed on breast milk or infant formula without LCPUFA supplementation.     

高职院校绩效工资制度存在的问题及对策——以武汉某职院人事分配制度为背景

为了最大程度地调动教职工的积极性和创造性,提高办学质量和办学效率,高职院校纷纷进行人事分配制度改革。由于高职院校存在着教师教育教学活动的投入难以可靠计量、教育教学活动的产出难以可靠测量以及教学效果难以可靠评估等特殊性,导致高校绩效工资制度的改革难以达到"效率优先,兼顾公平"的预期效果。本文以武汉某职院人事分配制度的改革方案为背景,由个别到一般,分析高职院校绩效工资改革存在的不足,并就如何进一步优化高职院校绩效工资制度改革提出意见和建议。     

地聚合物水泥路面快速修补材料性能研究

针对水泥路面修补后开放交通时间偏长、耐久性较差和施工工艺复杂的问题,采用固体复合激发剂、粉煤灰和偏高岭土及其他外加剂制备一种地聚合物水泥快速修补材料,并对其混凝土力学性能、界面粘结性能、收缩率、抗冻性能进行测试。结果表明,当掺入10%的复合激发剂和10%的普通硅酸盐水泥时,地聚合物水泥的早期力学性能达到最优,所制备的地聚合物水泥混凝土修补材料具有快凝早强和优良的耐久性等特点,扫描电子显微镜(SEM)微观分析表明,由于碱激发反应和未激发粉煤灰的填充效应,地聚合物水泥混凝土具有致密的微观结构。     

基于序列运算理论的孤立微电网经济运行模型

针对风力和光伏发电出力的随机性,提出一种基于机会约束规划的孤立微电网经济运行模型。鉴于部分机会约束难以转化成确定性约束,根据序列运算理论,利用可再生能源出力的概率分布生成概率性序列,使模型中的机会约束转化成确定性约束的等价类。因此模型具有混合整数规划的线性结构,可兼容如TOMLAB类的优化工具求解。仿真结果表明,与随机模拟求解相比,该方法具有计算速度快且结果稳定的优势。另外,为了考察概率性序列的合理性,还讨论了不同离散化步长对计算结果的影响,并进一步验证了所提出方法的有效性。     

湖北电网输电线路大负荷试验研究

在2007年夏季负荷高峰期间对湖北省电力公司的两条220kV卡口线路高楼线、郭楼线进行了大负荷考核试验,验证了提高线路输送能力理论研究的结果,同时积累了宝贵的经验,可以在今后类似的试验中加以推广。     

一种6信道声光调制器

介绍了一种6信道声光调制器。通过在换能器上制备电极阵列,设计独立的阻抗匹配电路和电输入端口,将6个声光互作用单元集成在一起,实现了对6束激光的并行、独立调制。经测试,该6信道声光调制器工作波长为355 nm,工作频率为220 MHz,单信道衍射效率＞75%,全局串扰＜0.5%。