DESIGN AND IMPLEMENTATION OF STATE OBSERVERS IN REACTIVE DISTILLATION

This study demonstrates that state observers can be developed and applied to infer the composition profiles of reactive distillation columns from noise-contaminated temperature measurements. The design and implementation of a Kalman filter (KF) and a Luenberger observer (LO) are carried out, and their performances are quantitatively assessed. The reliability, accuracy, and robustness of the two designs method are examined and compared quantitatively. The design and implementation of a Luenberger observer are simpler and easier to carry out than those of a Kalman filter. On the other hand, a Kalman filter is found to be more robust to a noisy measurements, erroneous initial estimates, and model uncertainties. A Luenberger observer could be used for composition estimation of reactive distillation when an ideal model of the system can reasonably approximate the real system; otherwise, a Kalman filter is recommended to be applied in more practical situations.     

Chemical composition and nutritional quality of tehineh (sesame butter)

The chemical composition and nutritional quality of tehineh, a paste of dehulled roasted sesame seeds, from Saudi Arabia and other countries, were studied. Results showed 24.7% protein, 58.9% fat, 2.3% fiber, 3.0% ash and <1.0% moisture. The tehineh contained relatively high amounts (mg/100 g) of P (692), Mg (362), Fe (7.19), Cu (1.96), Mn (1.46) and Zn (7.82) and low amounts of Ca (61) and Se (0.05). Gas-liquid chromatography (GLC) analysis of the oil revealed percentages of 42.4 for oleic, 39.7 for linoleic, 9.8 for palmitic and 6.4 for stearic acid. Lysine was the only limiting amino acid with a chemical score of 64, while the sulphur-containing amino acids (methionine + cystine) and tryptophan were present in amounts exceeding the requirement of the FAO/WHO (1973) reference protein. The in vitro protein digestibility (IVPD) value of 83.3% and the calculated protein efficiency ratio (C-PER), 2.14, were slightly higher than those of sesame seeds and both values were lower than the IVPD and C-PER of 90.0% and 2.50, respectively, for ANRC casein.     

Multimedia framework to support eHealth applications

Limited bandwidth resources lead to a number of challenges especially for eHealth applications, which are communicated over IP and wireless networks. These multimedia services include high-resolution videos and have very large file sizes that require a high level of compression to overcome this limitation. Therefore, there is an acute demand for the research community to provide an efficient multimedia framework to encode medical videos with high quality specifically under the conditions of an error-prone environment. Both an affordable delivery framework and effective coding techniques are extremely desirable for the delivery of high-quality eHealth video applications for transmission over heterogeneous networks and devices. In this paper, we propose and demonstrate a multimedia framework to support eHealth applications, which has an improved coding scheme that uses an SVC-scalable extension of MPEC-4 AVC/H.264. Simulation results show that the proposed scheme achieves a significant improvement in terms of the PSNR-Y gain and reduces the picture quality degradation caused by artifacts and distortions, compared to the existing scheme.     

Assessing tropical cyclone impacts using object-based moderate spatial resolution image analysis: a case study in Bangladesh

The environmental and societal impacts of tropical cyclones could be reduced using a range of management initiatives. Remote sensing can be a cost effective, accurate, and potential tool for mapping the multiple impacts caused by tropical cyclones using high-to-moderate spatial resolution (5–30 m) satellite imagery to provide data on the following essential parameters – evacuation, relief, and management of natural resources. This study developed and evaluated an approach for assessing the impacts of tropical cyclones through object-based image analysis and moderate spatial resolution imagery. Pre- and post-cyclone maps of artificial and natural features are required for assessing the overall impacts in the landscape that could be acquired by mapping specific land cover types. We used the object-based approach to map land-cover types in pre- and post-cyclone Satellite Pour l’Observation de la Terre (SPOT) 5 image data and the post-classification comparison technique to identify changes in the particular features in the landscape. Cyclone Sidr (2007) was used to test the applicability of this approach in Sarankhola Upazila in Bangladesh. The object-based approach provided accurate results for classifying features from pre- and post-cyclone satellite images with an overall accuracy of 95.43% and 93.27%, respectively. Mapped changes identified the extent, type, and form of cyclone induced impacts. Our results indicate that 63.15% of the study area was significantly affected by cyclone Sidr. The majority of mapped damage was found in vegetation, cropped lands, settlements, and infrastructure. The damage results were verified through the high spatial resolution satellite imagery, reports and pictures that were taken after the cyclone. The methods developed may be used in future to assess the multiple impacts caused by tropical cyclones in Bangladesh and other similar environments for the purposes of tropical cyclone disaster management.     

A Single Injection of NTG-101 Reduces the Expression of Pain-Related Neurotrophins in a Canine Model of Degenerative Disc Disease

Background: Tissue sources of pain emanating from degenerative discs remains incompletely understood. Canine intervertebral discs (IVDs) were needle puncture injured, 4-weeks later injected with either phosphate-buffered saline (PBS) or NTG-101, harvested after an additional fourteen weeks and then histologically evaluated for the expression of NGFr, BDNF, TrkB and CALCRL proteins. Quantification was performed using the HALO automated cell-counting scoring platform. Immunohistochemical analysis was also performed on human IVD tissue samples obtained from spinal surgery. Immunohistochemical analysis and quantification of neurotrophins and neuropeptides was performed using an in vivo canine model of degenerative disc disease and human degenerative disc tissue sections. Discs injected with NTG-101 showed significantly lower levels of Nerve Growth Factor receptor (NGFr/TrkA, p = 0.0001), BDNF (p = 0.009), TrkB (p = 0.002) and CALCRL (p = 0.008) relative to PBS injections. Human IVD tissue obtained from spinal surgery due to painful DDD show robust expression of NGFr, BDNF, TrkB and CALCRL proteins. A single intradiscal injection of NTG-101 significantly inhibits the expression of NGFr, BDNF, TrkB and CALCRL proteins in degenerative canine IVDs. These results strongly suggest that NTG-101 inhibits the development of neurotrophins that are strongly associated with painful degenerative disc disease and may have profound effects upon the management of patients living with discogenic pain.     

Pivotal Role of Phytohormones and Their Responsive Genes in Plant Growth and Their Signaling and Transduction Pathway under Salt Stress in Cotton

The presence of phyto-hormones in plants at relatively low concentrations plays an indispensable role in regulating crop growth and yield. Salt stress is one of the major abiotic stresses limiting cotton production. It has been reported that exogenous phyto-hormones are involved in various plant defense systems against salt stress. Recently, different studies revealed the pivotal performance of hormones in regulating cotton growth and yield. However, a comprehensive understanding of these exogenous hormones, which regulate cotton growth and yield under salt stress, is lacking. In this review, we focused on new advances in elucidating the roles of exogenous hormones (gibberellin (GA) and salicylic acid (SA)) and their signaling and transduction pathways and the cross-talk between GA and SA in regulating crop growth and development under salt stress. In this review, we not only focused on the role of phyto-hormones but also identified the roles of GA and SA responsive genes to salt stress. Our aim is to provide a comprehensive review of the performance of GA and SA and their responsive genes under salt stress, assisting in the further elucidation of the mechanism that plant hormones use to regulate growth and yield under salt stress.     

Discovery of Five New Ethylene-Forming Enzymes for Clean Production of Ethylene in E. coli

Ethylene is an essential platform chemical with a conjugated double bond, which can produce many secondary chemical products through copolymerisation. At present, ethylene production is mainly from petroleum fractionation and cracking, which are unsustainable in the long term, and harmful to our environment. Therefore, a hot research field is seeking a cleaner method for ethylene production. Based on the model ethylene-forming enzyme (Efe) {"type":"entrez-protein","attrs":{"text":"AAD16440.1","term_id":"4323597","term_text":"AAD16440.1"}}AAD16440.1 (6vp4.1.A) from Pseudomonas syringae pv. phaseolicol, we evaluated five putative Efe protein sequences using the data derived from phylogenetic analyses and the conservation of their catalytic structures. Then, pBAD expression frameworks were constructed, and relevant enzymes were expressed in E. coli BL21. Finally, enzymatic activity in vitro and in vivo was detected to demonstrate their catalytic activity. Our results show that the activity in vitro measured by the conversion of α-ketoglutarate was from 0.21–0.72 μmol ethylene/mg/min, which varied across the temperatures. In cells, the activity of the new Efes was 12.28–147.43 μmol/gDCW/h (DCW, dry cellular weight). Both results prove that all the five putative Efes could produce ethylene.