Stoichiometric dye-binding procedure for the determination of the reactive lysine content of soya bean protein

A new analytical method has been developed for the determination of the reactive lysine content of soya bean protein. The method is based on the reaction of the free basic groups of the protein with 1-phenylazo-2 naphthol-6,8 disulphonic acid. With regard to the stoichiometry of the procedure, it has been proved, contrary to earlier reports, that the basic amino acids, histidine, arginine and lysine, each combine with one mole of the dye. After acylation with propionic anhydride lysine alone loses its dye reactivity. The usefulness of the proposed method has been demonstrated by the determination of the reactive lysine content of several untreated, heat-treated and acid-treated soya bean samples. The results show that heat damage of about 5% in reactive lysine content can be measured in 1·5 h with good reproducibility.     

The joint movement of pebbles in solving the ($$N^2-1$$)-puzzle suboptimally and its applications in rule-based cooperative path-finding

The present paper deals with the problem of solving the (\(n^2 - 1\))-puzzle and cooperative path-finding (CPF) problems sub-optimally by rule-based algorithms. To solve the puzzle, we need to rearrange \(n^2 - 1\) pebbles in the \(n \times n\)-sized square grid using one vacant position to achieve the goal configuration. An improvement to the existing polynomial-time algorithm is proposed and experimentally analyzed. The improved algorithm represents an attempt to move pebbles in a more efficient way compared to the original algorithm by grouping them into so-called snakes and moving them together as part of a snake formation. An experimental evaluation has shown that the snakeenhanced algorithm produces solutions which are 8–9 % shorter than the solutions generated by the original algorithm. Snake-like movement has also been integrated into the rule-based algorithms used in solving CPF problems sub-optimally, which is a closely related task. The task in CPF consists in moving a group of abstract robots on an undirected graph to specific vertices. The robots can move to unoccupied neighboring vertices; no more than one robot can be placed in each vertex. The (\(n^2 - 1\))-puzzle is a special case of CPF where the underlying graph is a 4-connected grid and only one vertex is vacant. Two major rule-based algorithms for solving CPF problems were included in our study—BIBOX and PUSH-and-SWAP (PUSH-and-ROTATE). The use of snakes in the BIBOX algorithm led to consistent efficiency gains of around 30 % for the (\(n^2 - 1\))-puzzle and up to 50 % in for CPF problems on biconnected graphs with various ear decompositions and multiple vacant vertices. For the PUSH-and-SWAP algorithm, the efficiency gain achieved from the use of snakes was around 5–8 %. However, the efficiency gain was unstable and hardly predictable for PUSH-and-SWAP.     

A posteriori optimization of parameters in stabilized methods for convection–diffusion problems – Part I

Stabilized finite element methods for convection-dominated problems require the choice of appropriate stabilization parameters. From numerical analysis, often only their asymptotic values are known. This paper presents a general framework for optimizing stabilization parameters with respect to the minimization of a target functional. Exemplarily, this framework is applied to the SUPG finite element method and the minimization of a residual-based error estimator, an error indicator, and a functional including the crosswind derivative of the computed solution. Benefits of the basic approach are demonstrated by means of numerical results.     

Design and analysis of a bandwidth management framework forATM-based broadband ISDN

When designing and configuring an ATM-based B-ISDN, it remains difficult to guarantee the quality of service (QoS) for different service classes, while still allowing enough statistical sharing of bandwidth so that the network is efficiently utilized. These two goals are often conflicting. Guaranteeing QoS requires traffic isolation, as well as allocation of enough network resources (e.g., buffer space and bandwidth) to each call. However, statistical bandwidth sharing means the network resources should be occupied on demand, leading to less traffic isolation and minimal resource allocation. The authors address this problem by proposing and evaluating a network-wide bandwidth management framework in which an appropriate compromise between the two conflicting goals is achieved. Specifically, the bandwidth management framework consists of a network model and a network-wide bandwidth allocation and sharing strategy. Implementation issues related to the framework are discussed. For real-time applications the authors obtain maximum queuing delay and queue length, which are important in buffer design and VP (virtual path) routing     

Determination of PCBs in fatty tissues by means of several detection techniques

Difficulties with harmonization of analytical procedures and consequently poor comparability of generated data represent in the Czech Republic the main reason for the delay in issuing of updated legislation for polychlorobiphenyls (PCBs) in foodstuffs. This study draws attention to possible errors (overestimations) that can occur during routine determination of these residues in the fat portion of biotic matrices. We demonstrate the gas Chromatographic (GC) conditions under which interfering contaminants such as phthalates and/or chlorinated pesticides can be separated from analytes. Discussion is focused on the advantages and drawbacks of GC with an electron capture detector, GC mass spectrometry and GC with an atomic emission detector. Various approaches used for calculation of PCBs contents are compared.

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Bestimmung der PCB in Fettgeweben mittels verschiedener Detektionstechniken

Zusammenfassung Das Problem mit der Harmonisierung der analytischen Verfahren und der geringen Vergleichbarkeit der Angaben ist ein Hauptgrund der Verspätung in der neuen Legislative für PCB in Lebensmitteln in der Tschechischen Republik. Die Arbeit beschreibt die möglichen Fehler (Falsch-positive Fehler), die durch die routinierte Bestimmung von PCB-Rückständen in Fettanteilen der biologischen Materialien auftreten können. Es werden die gaschromatographischen Bedingungen beschrieben, unter denen die Interferenzen, wie z. B. Phthalaten und chlorierten Pesticiden, von Stoffen abgetrennt werden können. Die Vorteile und Nachteile der Methoden GC-ECD, GC-MS und GC-AED werden diskutiert. Die verschiedenen für die Kalkulation des PCB-Gehaltes angewendeten Annäherungen werden miteinander verglichen.

     

Influence of different SiC surface treatments performed prior to Ni ohmic contacts formation

This work is dealing with the influence of surface treatment on ohmic contacts to hexagonal N-type SiC with medium doping level. The contact materials were Ni and Ni₂Si. The structures had to be annealed at high temperatures in order to reach ohmic behavior. A number of surface treatment methods were tested: wet cleaning, plasma etching, intentional oxidation with etching, H₂ annealing and their combinations. After some types of cleaning, the SiC surface was immediately analysed using the XPS method. The results of the analyses showed that the composition of the surface was not much influenced by these treatments. At lower annealing temperatures (approx. up to 850 °C) the prepared contacts showed Schottky behavior with large scatter of parameters. After annealing at approx. 960 °C, where the onset of ohmic behavior is expected, the structures were truly ohmic and of good parameters. Cleaning methods had just a negligible influence on the electrical parameters of the ohmic contacts. An explanation for these observed facts is suggested: Although - already on the basis of the XPS results - we could speak about a negligible influence of the cleaning onto the contact parameters, there might come across also other mechanisms coming from interaction of contact materials with SiC, which caused similar behavior of ohmic contacts on differently treated surfaces.     

Blind separation of piecewise stationary non-Gaussian sources

We address independent component analysis (ICA) of piecewise stationary and non-Gaussian signals and propose a novel ICA algorithm called Block EFICA that is based on this generalized model of signals. The method is a further extension of the popular non-Gaussianity-based FastICA algorithm and of its recently optimized variant called EFICA. In contrast to these methods, Block EFICA is developed to effectively exploit varying distribution of signals, thus, also their varying variance in time (nonstationarity) or, more precisely, in time-intervals (piecewise stationarity). In theory, the accuracy of the method asymptotically approaches Cramér–Rao lower bound (CRLB) under common assumptions when variance of the signals is constant. On the other hand, the performance is practically close to the CRLB even when variance of the signals is changing. This is demonstrated by comparing our algorithm with various methods that are asymptotically efficient within ICA models based either on the non-Gaussianity or the nonstationarity. The benefit of our algorithm is demonstrated by examples with real-world audio signals.     

A high average power dual slab Nd:glass zigzag laser system

A dual slab Nd:LHG5 glass zigzag optical path laser was constructed with single-sided pumping and tested. A single slab (0.63 times 5.6 times 35cm) produced 80 W in an oscillator with an accompanying distortion of only 3 fringes (632.8 nm) as measured by a single-pass interferogram through the slab. Performance was achieved by producing uniform pumping and cooling of the slabs, and by controlling the distortion at the slab ends with pump shields.     

The effects of breed,sex, intramuscular fat and ultimate pH on pork tenderness

A study was conducted to evaluate the influence of breed and sex of animals on tenderness of pork. For evaluation, carcasses of pure breed barrows and gilts of Large White, Czech pig meat, Landrace and Duroc were used. For laboratory investigations, the samples of musculus longissimus lumborum et thoracis (20 mm thick) were taken from the same place, at the first cruciate vertebra 48 h post mortem. Seven days post mortem, the texture of heat-processed meat was measured objectively by the shear method on Instron equipped with Warner–Bratzler shear device. Meat tenderness and juiciness were also evaluated sensorially. It was observed that the tenderness of cooked meat is influenced by breed (the tenderest meat was from Duroc breed). The sex of animals has significant effect on meat tenderness too. At the same time, the effects of pH 45 minutes (pH₄₅) and 24 hours (pH₂₄) post mortem and intramuscular fat content on meat tenderness were evaluated. Correlations were found between shear force (tenderness) and intramuscular fat content and also between shear force and pH value 45 min post mortem. The shear force decreased with increasing intramuscular fat content and with increasing pH₄₅ value. Sensory evaluation of tenderness confirmed the objectively measured data.     

Cultivar differences of total anthocyanins and anthocyanidins in red and purple-fleshed potatoes and their relation to antioxidant activity

Total anthocyanins (TAC) and individual anthocyanidins (AN) after hydrolysis were measured in 15 red and purple-fleshed potato cultivars produced in five different locations in the Czech Republic and a new cultivar Blaue St. Galler from Switzerland. It was found that TAC, expressed as cyanidin content, varied between 0.7 mg 100 g⁻¹ FW (cv. Blue Congo) and 74.3 mg 100 g⁻¹ FW (cv. Blaue Ludiano). Major differences in cultivars were found for AN relative abundance. For cv. Highland Burgundy Red a high proportion of pelargonidin (98.7%) was characteristic, whereas cv. British Columbia Blue contained almost exclusively cyanidin. Cultivars Violette and Vitelotte showed a relatively high content of malvidin. Cultivar Shetland Black differed from others with its higher content of peonidin (on average 36.7%). High petunidin abundance in the cultivars Valfi, Blue Congo, Salad Blue, Blaue St. Galler, Blaue Hindel Bank, Blaue Ludiano, Blaue Schweden, Farbe Kartoffel and Salad Red was found. TAC and AN contents highly corresponded with antioxidant activity (AA) determined with the ABTS, FRAP and DPPH assays in vitro. High AA was shown by the cultivars Vitelotte, Violette, Blaue Ludiano, Hafija, and Highland Burgundy Red. Increased height above sea level, higher annual sum of precipitation, and lower annual average temperatures caused higher AA and TAC. A high degree of hydroxylation and/or methoxylation of individual anthocyanidins could contribute in conjunction with other phenolics to high AA (peonidin, delphinidin and malvidin in the cultivars Blue Congo, Highland Burgundy Red and Shetland Black). Consequently, new red and purple-fleshed cultivars with high TAC and highly methoxylated and/or hydroxylated AN could be a promising source of favourable antioxidants in human nutrition.