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OBJECTIVE: To develop a simple assay using the silica wool filtration (SIFT) procedure to estimate percentage of acrosome-reacted (AR) sperm. SETTING: Private andrology laboratory. MATERIALS AND METHODS: Capacitated sperm processed by the SIFT procedure to remove nonviable sperm and those with broken membrane were acrosome reacted by induction with a calcium ionophore. Following the acrosome reaction, the sample was processed by the SIFT procedure. Sperm concentration and acrosomal status were also determined before and after the SIFT procedure. RESULTS: The SIFT procedure prevented AR sperm from filtering (mean +/- SD, 22.2 +/- 1.9 vs. 9.8 +/- 4.9%). The mean percent of sperm retained in the filter (SIFT assay: 61.8 +/- 21.5%) was significantly higher than the percent of AR sperm estimated by a staining technique (26.4 +/- 7.3%), but they were significantly correlated (r = .34) with each other. The filtration of capacitated sperm prior to induction of the acrosome reaction eliminated, or at least minimized, the possibility of falsely increasing the percent of AR sperm estimated by the SIFT assay. The higher estimate obtained by SIFT assay, therefore, suggests that it may be estimating sperm at various stages that are undergoing the acrosome reaction. CONCLUSION: The findings suggest that the SIFT assay could be used to estimate the percentage of AR spermatozoa.  相似文献   
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This paper presents the design of a robust control law for a class of nonlinear dynamical systems subjected to parametric uncertainty and simultaneous unknown, variable state and input delays. A novel controller is developed, which consists of a filtered tracking error and the integral of previous values of control input where the limits of integration are dependent on the known bound of the input delay. Lyapunov‐Krasovskii functionals–based stability analysis guarantees a global uniformly ultimately bounded tracking result where sufficient conditions on controller gains and maximum allowable delay are derived. The performance and robustness of the controller are evaluated by simulation on a two‐link robot manipulator for different combinations of time‐varying state and input delays.  相似文献   
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The primary heat transport system of 500 MWe Indian PHWR comprises of straight pipes, elbows and headers. A study was conducted to qualify piping system for leak before break. R-6 method was used to assess integrity of system for leakage size crack (LSC), the margins on crack initiation load and unstable crack growth loads. Option 2 (material specific failure assessment diagram), Category 3 (ductile tearing) analysis was used for straight pipes, elbows and header. In order to enhance the confidence in the analytical results, detailed sensitivity analysis was also performed. For sensitivity analysis, variation in material properties, LSC was considered. The effect of variation in temperature on material properties was also considered. Tensile and fracture properties used for base and weld material data were generated from pipe material obtained from 220 MWe Nuclear Power Plant, under construction.  相似文献   
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The loss of zero-voltage-switching (ZVS) of active switches has been a serious limitation of full-bridge (FBZVS) converters. Many techniques have been proposed in the past to extend the range of ZVS operation over the wider and also the full range of operation. However, in these techniques ZVS is achieved at the expense of additional conduction loss in active switches and losses in the auxiliary components. In this paper, the analysis for the additional losses in various full-range FBZVS DC-DC converters and their comparative evaluation is reported. Closed form expressions are derived for average value of device currents and losses. The loss curves for various topologies are plotted and compared. The analytical results are found to be consistent with the experimental efficiency tests performed on 500 W, 100 kHz prototype. It is concluded that a recently proposed new topology has the least penalty of additional losses.  相似文献   
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In this work, an attempt has been made to synthesize well-distributed stable selenium (Se) particles of nanosize dimensions via an aqueous micellar solution by the assistance of surfactants of two different polarities (anionic, sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and cationic, hexadecyltrimethylammonium bromide (CTAB)). The morphology of the particles was examined with transmission electron microscopy (TEM). X-ray analysis reveals that the particles have a monoclinic structure. The band gap of the particles was determined from UV-visible optical spectroscopic results. The size variation was estimated by employing a quantum confinement effect equation. The evolution of the selenium nanoparticles in AOT and CTAB micellar media was corroborated with the time-dependent absorption spectra. The influence of hydrazine hydrate concentrations on the formation kinetics of Se nanoparticles was also investigated. The capping ability of the surfactants has been quantitatively evaluated from Fourier transform infrared (FTIR) studies.  相似文献   
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This paper is a generalization of the recently developed techniques of initial excitation (IE)–based adaptive control with an introduction to the definition of semi‐initial excitation (semi‐IE), a still more relaxed notion than IE. Classical adaptive controllers typically ensure Lyapunov stability of the extended error dynamics (tracking error + parameter estimation error) and asymptotic tracking, while requiring a stringent condition of persistence of excitation (PE) for parameter convergence. Of late, the authors have proposed a new adaptive control architecture, which guarantees parameter convergence under the online‐verifiable IE condition leading to exponential stability of the extended error dynamics. In earlier works, it has been established that the IE condition is significantly milder than the classical PE condition. The current work further slackens the excitation condition by proposing the concept of semi‐IE. The proposed adaptive controller is proved to ensure convergence of the parameter estimation error to a lower‐dimensional manifold under the weaker semi‐IE condition, while the stronger condition of IE guarantees convergence of the parameter estimation error to zero. The designed algorithm is shown to improve transient response of tracking error sufficiently in contrast to conventional adaptive controllers.  相似文献   
10.
The seismic stability of the Åknes rock slope, western Norway, was analysed by using the distinct element code UDEC (Universal Distinct Element Code). The slope poses a threat to the region as a sudden failure may cause a destructive tsunami in the fjord. The dynamic input was based on earthquakes with return periods of 100 and 1000 years, and in most models the input shear wave was a harmonic function (sine wave). Models with depths of the sliding surface up to 200 m and with ground water conditions derived from site investigations were analysed, as well as models with ground water conditions assumed from possible future draining of the slope. The analyses indicate that an earthquake with a return period of 1000 years is likely to trigger sliding to great depth in the slope at the present ground water conditions and that the slope will be stable if it is drained. The analyses also indicate that sliding is not likely to be triggered by an earthquake with a return period of 100 years at the present ground water conditions.  相似文献   
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