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31.
Force-distance curves by AFM 总被引:1,自引:0,他引:1
Cappella B. Baschieri P. Frediani C. Miccoli P. Ascoli C. 《IEEE engineering in medicine and biology magazine》1997,16(2):58-65
Since 1989, the atomic force microscope (AFM) has emerged as a useful tool in studying surface interactions by means of force-distance curves, and a great deal of work has been carried out on both its theoretical and experimental issues. AFM is able to acquire force-distance curves from every kind of surface, with high lateral (1 Å), vertical (0.1 Å) and force (1 pN) resolution. Moreover, the study of force-distance curves provides a deeper knowledge of the physics of contact, and hence of all the phenomena connected with AFM imaging techniques. In this article, we review force-distance curve theory, and show through curves acquired with a home-made microscope the main surface interactions that can be revealed 相似文献
32.
G Gaidano D Capello AM Cilia A Gloghini T Perin S Quattrone A Migliazza F Lo Coco G Saglio V Ascoli A Carbone 《Canadian Metallurgical Quarterly》1999,24(1):16-23
A new type of antimicrobial peptide, snakin-1 (SN1), has been isolated from potato tubers and found to be active, at concentrations < 10 microM, against bacterial and fungal pathogens from potato and other plant species. The action of SN1 and potato defensin PTH1 was synergistic against the bacterium Clavibacter michiganensis subsp. sepedonicus and additive against the fungus Botrytis cinerea. Snakin-1 causes aggregation of both gram-positive and gram-negative bacteria. The peptide has 63 amino acid residues (M(r) 6,922), 12 of which are cysteines, and is unrelated to any previously isolated protein, although it is homologous to amino acid sequences deduced from cloned cDNAs that encode gibberellin-inducible mRNAs and has some sequence motifs in common with kistrin and other hemotoxic snake venoms. A degenerate oligonucleotide probe based on the internal sequence CCEECKC has been used to clone an SN1 cDNA. With the cDNA used as probe, one copy of the StSN1 gene per haploid genome has been estimated and expression of the gene has been detected in tubers, stems, axillary buds, and young floral buds. Expression levels in petals and carpels from fully developed flowers were much higher than in sepals and stamens. The expression pattern of gene StSN1 suggests that protein SN1 may be a component of constitutive defense barriers, especially those of storage and reproductive plant organs. 相似文献
33.
Mannoni A. Quercioli F. Tiribilli B. Ascoli C. Baschieri P. Frediani C. 《Lightwave Technology, Journal of》1998,16(3):388-394
An hybrid atomic force-scanning near-field optical microscope (AFM-SNOM) has been realized starting from a home-built AFM. The instrument uses a tetrahedral SiN tip for force and near field detection and is designed to provide, besides simple imaging, a full three-dimensional (3-D) mapping of force, friction and light intensity on the sample. High-resolution optical images of dielectric samples are presented together with curves describing the behavior of the collected optical intensity as a function of tip-object distance. AFM images and force-distance curves are reported as well and are compared with those obtained from the optical channel. Subwavelength features of the samples can be easily appreciated in both kinds of images, and the refractive index of the object can be computed from the attenuation constant of the tunneling light detected by the tip, with the AFM curve providing a convenient way of detecting the contact point. The spatial resolution of this kind of measurement is far better than that obtainable with any other index-measuring device. Homogeneous glass samples as well as monomode channel waveguides were analyzed in our experiments. The refractive index of several different prisms, made either of BK7 (n=1.519 at λ=532 nm) or of SF58 (n=1.932) glass, could be measured with our near-field technique. As for the channel waveguides, the accuracy of our measurements lets us detect their presence and lateral extension in the substrate but is not yet sufficient to allow the reconstruction of their index profile 相似文献
34.
G Smulevich M Paoli G De Sanctis AR Mantini F Ascoli M Coletta 《Canadian Metallurgical Quarterly》1997,36(3):640-649
Resonance Raman (RR), electronic absorption, and circular dichroism (CD) spectroscopies of the ferric, ferrous, and ferrous-CO forms of horseradish peroxidase (HRP-C) at pH 3.1 are reported. The CD spectra in the UV region show only a small decrease in the alpha-helical content upon pH lowering, whereas dramatic changes are observed in the Soret region. The final form of ferric HRP-C is 5-coordinate high-spin heme whose histidine ligand is replaced by a water ligand with a polar character. The electronic and CD spectra show the presence of an intermediate form with a 6-coordinate heme. Therefore, the cleavage of the proximal Fe-imidazole bond is preceded by the binding of a distal water molecule. For the ferrous form of HRP-C, the pH-dependence of the absorption spectra revealed only the native form in the range pH 5-7 and an unfolded form with a Soret maximum at 383 nm at pH 3.1. An intermediate state, characterized by a Soret maximum at 424 nm, was observed only in a transient way, within a few milliseconds. A metastable and a final species are observed also for the ferrous-CO complex at pH 3.1, as proved by isosbestic points in the electronic absorption spectra. The two forms show different RR nu(Fe-C) and IR nu(CO) modes. The metastable form corresponds to a heme where histidine is replaced by water. The final form is due to the displacement of the water ligand by the proximal histidine. We propose a kinetic model to account for our results at pH 3.1 for the ferric, ferrous, and ferrous-CO forms. 相似文献
35.
Tal A 《Environmental science & technology》1997,31(10):470A-476A
Risk Assessment. 相似文献
36.
The essential difference between classical multiple sclerosis and both Devic's neuromyelitis optica and acute disseminated encephalomyelitis are outlined. Advances in identifying the multiple gene mutations responsible for adrenoleukodystrophy and possible mechanisms for the genotypic/phenotypic variability are reviewed. 相似文献
37.
Niels D. Naimon Jerzy Walczyk James S. Babb Oleksandr Khegai Xuejiao Che Leeor Alon Ravinder R. Regatte Ryan Brown Prodromos Parasoglou 《Magma (New York, N.Y.)》2017,30(3):281-289
Objective
To develop a low-cost pedal ergometer compatible with ultrahigh (7 T) field MR systems to reliably quantify metabolic parameters in human lower leg muscle using phosphorus magnetic resonance spectroscopy.Materials and methods
We constructed an MR compatible ergometer using commercially available materials and elastic bands that provide resistance to movement. We recruited ten healthy subjects (eight men and two women, mean age ± standard deviation: 32.8 ± 6.0 years, BMI: 24.1 ± 3.9 kg/m2). All subjects were scanned on a 7 T whole-body magnet. Each subject was scanned on two visits and performed a 90 s plantar flexion exercise at 40% maximum voluntary contraction during each scan. During the first visit, each subject performed the exercise twice in order for us to estimate the intra-exam repeatability, and once during the second visit in order to estimate the inter-exam repeatability of the time constant of phosphocreatine recovery kinetics. We assessed the intra and inter-exam reliability in terms of the within-subject coefficient of variation (CV).Results
We acquired reliable measurements of PCr recovery kinetics with an intra- and inter-exam CV of 7.9% and 5.7%, respectively.Conclusion
We constructed a low-cost pedal ergometer compatible with ultrahigh (7 T) field MR systems, which allowed us to quantify reliably PCr recovery kinetics in lower leg muscle using 31P-MRS.38.
39.
Keren Nitzan Leah Ellenbogen Ziv Bentulila Dekel David Motty Franko Emanuela P. Break Michal Zoharetz Alon Shamir Yosef Sarne Ravid Doron 《International journal of molecular sciences》2022,23(16)
Alzheimer’s disease (AD) is the most common form of dementia, but there is still no available treatment. Δ9-tetrahydrocannabinol (THC) is emerging as a promising therapeutic agent. Using THC in conventional high doses may have deleterious effects. Therefore, we propose to use an ultra-low dose of THC (ULD-THC). We previously published that a single injection of ULD-THC ameliorated cognitive functioning in several models of brain injuries as well as in naturally aging mice. Here, 5xFAD AD model mice received a single treatment of ULD-THC (0.002 mg/kg) after disease onset and were examined in two separate experiments for cognitive functions, neurotropic, and inflammatory factors in the hippocampus. We show that a single injection of ULD-THC alleviated cognitive impairments in 6- and 12-month-old 5xFAD mice. On the biochemical level, our results indicate an imbalance between the truncated TrkB receptor isoform and the full receptor, with AD mice showing a greater tendency to express the truncated receptor, and ULD-THC improved this imbalance. We also investigated the expression of three AD-related inflammatory markers and found an ameliorating effect of ULD-THC. The current research demonstrates for the first time the beneficial effects of a single ultra-low dose of THC in a mouse model of AD after disease onset. 相似文献
40.
The control algorithm based on the uncertainty and disturbance estimator (UDE) is a robust control strategy and has received wide attention in recent years. In this paper, the two‐degree‐of‐freedom nature of UDE‐based controllers is revealed. The set‐point tracking response is determined by the reference model, whereas the disturbance response and robustness are determined by the error feedback gain and the filter introduced to estimate the uncertainty and disturbances. It is also revealed that the error dynamics of the system is determined by two filters, of which one is determined by the error feedback gain and the other is determined by the filter introduced to estimate the uncertainty and disturbances. The design of these two filters are decoupled in the frequency domain. Moreover, after introducing the UDE‐based control, the Laplace transform can be applied to some time‐varying systems for analysis and design because all the time‐varying parts are lumped into a signal. It has been shown that, in addition to the known advantages over the time‐delay control, the UDE‐based control also brings better performance than the time‐delay control under the same conditions. Design examples and simulation results are given to demonstrate the findings. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献