Encapsulation of albumin in self-assembled layer-by-layer microcapsules: comparison of co-precipitation and adsorption techniques

Objective: The objective of this study is to prepare and characterize polymeric self-assembled layer-by-layer microcapsules (LbL-MC) to deliver a model protein, bovine serum albumin (BSA). The aim is to compare the BSA encapsulation in LbL-MC using co-precipitation and adsorption methods.

Materials and methods: In co-precipitation method, BSA was co-precipitated with growing calcium carbonate particles to form a core template. Later, poly(styrene sulfonate) and poly(allylamine hydrochloride) were sequentially adsorbed onto the CaCO₃ templates. In adsorption method, preformed LbL-MC were incubated with BSA and encapsulation efficiency is optimized for pH and salt concentration. Free and BSA-encapsulated LbL-MC were characterized using Zetasizer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy and differential scanning calorimeter. Later, in vitro release studies were performed using dialysis membrane method at pH 4, 7.4 and 9.

Results and discussion: Results from Zetasizer and SEM showed free LbL-MC with an average size and zeta-potential of 2.0?±?0.6?μm and 8.1?±?1.9?mV, respectively. Zeta-potential of BSA-loaded LbL-MC was (–)7.4?±?0.7?mV and (–)5.7?±?1.0?mV for co-precipitation and adsorption methods, respectively. In adsorption method, BSA encapsulation in LbL-MC was found to be greater at pH 6.0 and 0.2?M NaCl. Co-precipitation method provided four-fold greater encapsulation efficiency (%) of BSA in LbL-MC compared with adsorption method. At pH 4, the BSA release from LbL-MC was extended up to 120?h. Polyacrylamide gel electrophoresis showed that BSA encapsulated in LBL-MC through co-precipitation is stable toward trypsin treatment.

Conclusion: In conclusion, co-precipitation method provided greater encapsulation of BSA in LbL-MC. Furthermore, LbL-MC can be developed as carriers for pH-controlled protein delivery.     

Effects of Ti-based catalysts and synergistic effect of SWCNTs-TiF3 on hydrogen uptake and release from MgH2

The present investigations are focused on the effect of different Ti-based catalysts (Ti, TiO₂, TiCl₃ and TiF₃) on de/re-hydrogenation characteristics of nanocrystalline MgH₂. Desorption temperature of milled MgH₂ lowers from 380 to 350, 340, 310 and 260 °C with the addition of Ti, TiO_2, TiCl₃ and TiF₃ respectively. The rehydrogenation characteristics are also improved through the deployment of Ti-based catalysts. Among all Ti based additives, TiF₃ is found to be the most effective catalyst for hydrogen sorption from nano MgH₂. The better catalytic effect of TiF₃ over other Ti-based catalyst can be explained on the basis of temperature programmed reduction (TPR) studies. TPR experiments performed for different Ti additives, reveals that there is no oxidation/reduction reaction below 400 °C except for TiF₃. The TPR profile of TiF₃ shows some oxidation/reduction reaction exhibits at 200 °C. In order to further improve the sorption characteristics and cyclability of TiF₃ catalyzed nano MgH₂, we have investigated the effect of SWCNTs in MgH₂+TiF₃ sample. De/rehydrogenation characteristics reveal the synergistic effect of SWCNTs and TiF₃ in MgH₂+TiF₃ sample. The details of the improvement in sorption behavior of MgH₂–TiF₃ in presence of SWCNTs are described and discussed.     

Development and gamma scintigraphy evaluation of gastro retentive calcium ion-based oral formulation: an innovative approach for the management of gastro-esophageal reflux disease (GERD)

Calcium chloride is an essential calcium channel agonist which plays an important role in the contraction of muscles by triggering calcium channel. First time hypothesized about its role in the treatment of GER (gastro-esophageal reflux) and vomiting disorder due to its local action. There are two objectives covered in this study as first, the development and optimization of floating formulation of calcium chloride and another objective was to evaluate optimized formulation through gamma scintigraphy in human subjects. Gastro retentive formulation of calcium chloride was prepared by direct compression method. Thirteen tablet formulations were designed with the help of sodium chloride, HPMC-K4M, and carbopol-934 along with effervescing agent sodium bicarbonate and citric acid. Formulation (F8) fitted best for Korsmeyer–Peppas equation with an R² value of 0.993. The optimized formulation was radiolabelled with ^99mTc-99?m pertechnetate for its evaluation by gamma scintigraphy. Gastric retention (6?h) was evaluated by gamma scintigraphy in healthy human subjects and efficacy of present formulation confirmed in GER positive human subjects. Gamma scintigraphy results indicated its usefulness in order to manage GERD. Stability studies of the developed formulation were carried out as per ICH guidelines for region IV and found out to be stable for 24?months.     

Elimination of current non-uniformity in carbon nanotube field emitters

Activation of abnormal emitting sites in Carbon Nano Tube (CNT) field emitters and their elimination is reported. CVD grown, patterned CNT was used as cathode for field emission studies. We encountered the problem of current non-uniformity in CNT cathode. This non-uniformity was attributed to abnormally active emitting sites during voltage ramp-up. The sudden increase in current resulted in region of positive slope in F–N curve, which can’t be explained by conventional F–N theory. Also the grown CNTs can be a mixture of metallic and semi conducting nature, which may cause deviation from the conventional F–N theory. We could eliminate abnormally active sites by electric field treatment, thereby increasing current uniformity and stability. The work is underway to understand the deviation in FN curve at high fields.     

Upgrading of Candelilla biocrude to hydrocarbon fuels by fluid catalytic cracking

Biomass has been recognized as a major world renewable energy source to supplement declining fossil fuel sources of energy. Biomass derived transportation fuels have not only the potential to replace conventional fuels but can also be utilized as blending components for improving the quality of these fuels. The biocrude obtained from Euphorbia antisyphilitica, identified as the most potential petrocrop was investigated as a potential source for liquid fuels. The feed was studied for yield conversion data under different catalyst to feed ratio at various temperatures. Maximum middle distillates selectivity was observed at catalyst/oil ratio 4 and 6 and reaction temperature 500 °C. The main constituents of the gaseous products are C₃, C₄ and C₅. The liquid fuels are highly aromatic with low olefinic content.     

Chelating ionophores based electrochemical sensor for Hg(II) ions

Poly(vinyl chloride) (PVC)-based membranes of pyrimidines, 1-(2′-aminoaryl)-4, 4, 6-trimethyl-1, 4, 5, 6-tetrahydro-6-hydroxypyrimidine-2-thiol (HPT) (R = −H, −CH₃, −OCH₃) with sodium tetraphenylborate (NaTPB) as an anion inhibitor and dibutylphthalate (DBP), dioctylphthalate (DOP), dibutyl (butyl) phosphonate (DBBP) and 1-chloro naphthalene (CN) as plasticizing solvent mediators were prepared and used as Hg²⁺ selective electrodes. Optimum performance was observed with the membrane having HPT(−OCH₃)−PVC−NaTPB−DOP in the ratio 1:33:1:65 (w/w). The electrode works well over a wide concentration range 5.0 × 10⁻⁶−1.0 × 10⁻¹ M (detection limit 1.0 ppm) with Nernstian compliance (29.5 mV decade⁻¹) between pH 2.5 and 4.0 with a fast response time of about 20 s. The selectivity coefficient values of the order of 0.001 for mono-, bi- and trivalent cations, indicate high selectivity for Hg²⁺ ions over these cations. Further, anions such as Cl⁻ and SO ₄ ²⁻ do not interfere in the functioning of Hg²⁺ sensor. The electrodes were used over a period of five months with good reproducibility (std. dev. ±0.2 mV) and the performance of the electrodes was found satisfactory even up to 10% nonaqueous medium. The electrode assembly was also evaluated for potentiometric determination of Hg²⁺.     

Dual gas sensing properties of graphene-Pd/SnO2 composites for H2 and ethanol: Role of nanoparticles-graphene interface

In the present work, role of palladium (Pd) and tin oxide (SnO₂) nanoparticles (NPs) deposited on graphene has been investigated in terms of dual gas sensing characteristics of ethanol and H₂ between two temperatures. The incorporation of nanoparticles into graphene has been observed which results a large change in the sensing response towards these gases. It is investigated that, incorporation of isolated Pd NPs on the graphene facilitates the room temperature sensing of H₂ gas with fast response and recovery time whereas, isolated SnO₂ NPs on graphene enables the detection of ethanol at 200 °C. However, combination of isolated Pd and SnO₂ NPs on graphene shows improved sensitivity and good selectivity towards H₂ and ethanol, usually not observed in chemiresistive gas sensors. Catalytic PdH interaction and corresponding change in work function of nanoparticles on hydrogenation resulting in modifications in electronic exchange between Pd, SnO₂ and graphene are responsible for the observed behavior. These results are important for developing a new class of chemiresistive type gas sensor based on change in the electronic properties of the graphene and NPs interfaces.     

Development of nanocolloidal gold based immunochromatographic assay for rapid detection of transgenic vegetative insecticidal protein in genetically modified crops

Vegetative insecticidal protein (Vip) is now being used for transgenic expression in several crops; conferring resistance against lepidopteron pests. A rapid, single step, sensitive and specific immunochromatographic (IC) strip test for the detection of recombinant Vip-S protein in the transgenic samples was developed. Polyclonal rabbit anti-Vip-S IgG conjugated to nanocolloidal gold served as a probe to detect Vip protein in test samples. The detection limit for the developed IC strip was 100 ng/ml (100 ppb) and on addition of gold enhancer the sensitivity increased to 1 ng/ml (1 ppb) of Vip-S protein. The assay was validated with transgenic brinjal samples. The assay time was less than 10 min, suitable for rapid on-site testing. No cross-reactivity was observed with other transgenic plant proteins employed for pest and weed management, i.e. Cry1Ac, Cry1Ab, and CP4-EPSPS. This on-site test offers rapid screening for a genetically modified crops having relatively new transgene (vip) entering the global market.     

Differential Coding for Non-Orthogonal Space-Time Block Codes with Non-Unitary Constellations over Arbitrarily Correlated Rayleigh Channels

In this paper, we propose a maximum likelihood (ML) decoder for differentially encoded full-rank square nonorthogonal space-time block codes (STBCs) using unitary or non-unitary signal constellations, which is also applicable to fullranked orthogonal STBC (OSTBC). As the receiver is jointly optimized with respect to the channel and the unknown data, it does not require any knowledge of channel power, signal power, or noise power to decode the signal, and the decision is purely based on two consecutively received data blocks. We analyze the effect of channel correlation on the performance of the proposed system in Rayleigh fading channels. Assuming a general correlation model, an upper bound of the pair-wise error probability (PEP) of the differential OSTBCs is derived. An approximate bound of the PEP for the differential nonorthogonal STBCs is also derived. We propose a precoder designing criterion for differential STBC over arbitrarily correlated Rayleigh channels. Precoding improves the system performance over the correlated Rayleigh MIMO channels. Our precoded differential codes differ from the previously proposed precoder designs for differential OSTBC in the following ways: 1) We propose a precoder design for arbitrarily correlated Rayleigh channels, whereas the previous work considers only for transmit correlation. 2) The previous work is only applicable to the OSTBCs with PSK constellations, whereas our precoder is applicable to any type of full-rank square STBCs with unitary and non-unitary signal constellations.