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排序方式: 共有842条查询结果,搜索用时 15 毫秒
61.
62.
Virus Matryoshka: A Bacteriophage Particle—Guided Molecular Assembly Approach to a Monodisperse Model of the Immature Human Immunodeficiency Virus 下载免费PDF全文
Pooja Saxena Li He Andrey Malyutin Siddhartha A. K. Datta Alan Rein Kevin M. Bond Martin F. Jarrold Alessandro Spilotros Dmitri Svergun Trevor Douglas Bogdan Dragnea 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(42):5862-5872
Immature human immunodeficiency virus type 1 (HIV‐1) is approximately spherical, but is constructed from a hexagonal lattice of the Gag protein. As a hexagonal lattice is necessarily flat, the local symmetry cannot be maintained throughout the structure. This geometrical frustration presumably results in bending stress. In natural particles, the stress is relieved by incorporation of packing defects, but the magnitude of this stress and its significance for the particles is not known. In order to control this stress, we have now assembled the Gag protein on a quasi‐spherical template derived from bacteriophage P22. This template is monodisperse in size and electron‐transparent, enabling the use of cryo‐electron microscopy in structural studies. These templated assemblies are far less polydisperse than any previously described virus‐like particles (and, while constructed according to the same lattice as natural particles, contain almost no packing defects). This system gives us the ability to study the relationship between packing defects, curvature and elastic energy, and thermodynamic stability. As Gag is bound to the P22 template by single‐stranded DNA, treatment of the particles with DNase enabled us to determine the intrinsic radius of curvature of a Gag lattice, unconstrained by DNA or a template. We found that this intrinsic radius is far larger than that of a virion or P22‐templated particle. We conclude that Gag is under elastic strain in a particle; this has important implications for the kinetics of shell growth, the stability of the shell, and the type of defects it will assume as it grows. 相似文献
63.
The accuracy of Moderate-resolution Imaging Spectroradiometer (MODIS) level 3 1 km land surface temperature (LST) products was assessed through long-term validation carried out in a mountainous site in Sierra Nevada, southeast Spain. A total of 1458 day and night thermal images, acquired by Terra and Aqua satellites during 2008, were processed and compared to ground-truth data recorded at the meteorological station of Robledal de Cañar with a frequency of one measurement every 10 min. The purpose of this investigation was to understand whether MODIS LST data can be used as input for climate models to be constructed for mountainous environments. Several trends in the MODIS LST data were observed, including the underestimation of daytime values and the overestimation of night-time values. Although all the data sets (Terra and Aqua, diurnal and nocturnal) showed high correlation coefficients with ground measurements, only night values maintained a relatively high accuracy of approximately 2°C of annual average error. Factors that may cause errors in the MODIS LST data, like acquisition angle, cloud, and snow cover, were analysed without conclusive results. High accuracy levels, i.e. close to 1°C, similar to other validation studies carried out over simpler and much more homogenous land-cover types such as cultivated fields, have been achieved for night images acquired during the summer months, thus making these datasets reliable for their use in climatic models over mountainous regions. 相似文献
64.
Bogdan Marinescu 《International journal of control》2013,86(10):1568-1589
In this paper it is shown how the robustness and the coordination of the voltage regulation actions for the transmission grid can be improved. Simpler approaches which ensure higher robustness and performances can be used if the control objectives are pursued at two hierarchical levels of different nature. Also, this is a way to coordinate means of control of different nature with a sufficient time and methodological separation in order to avoid negative mutual influence. At the first level, called the static level, optimal reachable set-points are computed for the second control level, called the dynamic level. The static level can be combined with the shunt reactive power compensation. The system non-linearities are taken into account at the static level while the dynamic level is a linear robust predictive control which takes into account the presence of asynchronous transmission delays. The predictive control strategy is based on the separation property; the output delays are handled using an original steady-state Kalman predictor of order equal to the length of the state of the system without delays. The robustness is improved at the dynamic level against uncertain delays, parametric uncertainties (like, e.g., moderate topological errors and load variations not taken into account in the control model) and unmodelled dynamics. The two-level organisation of the control allows, on one hand, to take into account the important evolutions of the system (like, e.g., large and known topological and load changes) and, on the other hand, a coherent hybrid reactive power control: the switched control of the grid shunt compensation for the reactive power is done at the static level while the reactive power injection provided by the generators is continuously handled at the dynamic level. This is a theoretical analysis of how concepts of automatic control and voltage regulation of power systems can be combined. To be applied as a control scheme, the results presented here should be adapted to a specific context (particularities of the power system and of the organisation of the power industry). They can be used, eventually in conjunction with other improvements, to existing horizontally-organised interconnections (in which all generators of a controlled region can be easily managed since owned by the same utility) or to face specific requirements of moving to the open access in the electric power industry like, e.g., tolerating simplified models in order to cover larger regions, taking into account the interaction between regions, recalibrating set-points, assisting human operator when necessary or facilitating implementation of mechanisms for the management of the reactive power based on price signals. 相似文献
65.
This paper proposes a system for the early automatic recognition of health problems that manifest themselves in distinctive
form of gait. Purpose of the system is to prolong the autonomous living of the elderly at home. When the system identifies
a health problem, it automatically notifies a physician and provides an explanation of the automatic diagnosis. The gait of
the elderly user is captured using a motion-capture system, which consists of body-worn tags and wall-mounted sensors. The
positions of the tags are acquired by the sensors and the resulting time series of position coordinates are analyzed with
machine-learning algorithms in order to recognize a specific health problem. Novel semantic features based on medical knowledge
for training a machine-learning classifier are proposed in this paper. The classifier classifies the user’s gait into: 1)
normal, 2) with hemiplegia, 3) with Parkinson’s disease, 4) with pain in the back and 5) with pain in the leg. The studies
of 1) the feasibility of automatic recognition and 2) the impact of tag placement and noise level on the accuracy of the recognition
of health problems are presented. The experimental results of the first study (12 tags, no noise) showed that the k-nearest
neighbors and neural network algorithms achieved classification accuracies of 100%. The experimental results of the second
study showed that classification accuracy of over 99% is achievable using several machine-learning algorithms and 8 or more
tags with up to 15 mm standard deviation of noise. The results show that the proposed approach achieves high classification
accuracy and can be used as a guide for further studies in the increasingly important area of Ambient Assisted Living. Since
the system uses semantic features and an artificial-intelligence approach to interpret the health state, provides a natural
explanation of the hypothesis and is embedded in the domestic environment of the elderly person; it is an example of the semantic
ambient media for Ambient Assisted Living. 相似文献
66.
This paper presents new digital measuring algorithms for generator and transformer protection. Two methods are proposed to make the estimation of amplitudes of the fundamental frequency component and the higher harmonics immune to frequency deviations. The first method is based on the software re-sampling of the physical data window and on the simultaneous iterative estimation of the frequency. The second algorithm employs the amplitude estimator having unique frequency response in the entire frequency spectrum and enables direct compensation for the frequency deviations. Also a new Volts/Hertz inverse-time algorithm is proposed which does not call for the direct measure of frequency. It relies on the second method and uses the amplitude estimator having the gain close to the inverse of the frequency; therefore, it acts as a direct amplitude-to-frequency estimator. The proposed algorithms were analyzed analytically and tested using EMTP simulations 相似文献
67.
Smolka Bogdan Malik Krystyna Malik Dariusz 《Journal of Real-Time Image Processing》2015,10(2):289-311
Journal of Real-Time Image Processing - In this paper, a novel approach to the problem of impulsive noise removal in color digital images is presented. The described switching filter is based on... 相似文献
68.
69.
Bogdan Štefane 《Catalysis Reviews》2014,56(1):82-174
Hydrogenation of prochiral ketones using chiral transition-metal catalysts represents the cleanest way to access enantiomerically enriched secondary alcohols, which are important building blocks in fine chemicals synthesis. Despite excellent activity, selectivity and compatibility of metal complexes with variety of functional groups, no universal catalysts exist. In this article we summarize the advances in catalyst systems for the asymmetric homogenous and heterogenous hydrogenation of ketones that have been made in past decade. The development of catalysts is oriented in reaching as high as activity with low catalyst loadings, using “greener’’ conditions, and ensuring good recyclability of catalysts. Even though ruthenium complexes represent the largest part of the catalysts, other metals rapidly penetrate this field. 相似文献
70.
Mariana Pinteala Valeria Harabagiu Cornelia Cotzur Marian N. Holerca Bogdan C. Simionescu 《Polymer Bulletin》1994,32(2):173-178
Summary Carboxyester- and carboxyamido-terminated polydimethylsiloxanes were obtained through the reaction of cyclic anhydrides (succinic, maleic and phthalic anhydride) with hydroxypropyl- and aminoalkyl-terminated polydimethylsiloxanes, respectively. The reactivity of the starting compounds and the influence of the reaction conditions on the characteristics of the final products are discussed.Part 1: Eur. Polym. J., in press 相似文献