Comparison of the eye's wave-front aberration measured psychophysically and with the Shack-Hartmann wave-front sensor

The Shack-Hartmann wave-front sensor offers many theoretical advantages over other methods for measuring aberrations of the eye; therefore it is essential that its accuracy be thoroughly tested. We assessed the accuracy of a Shack-Hartmann sensor by directly comparing its measured wave-front aberration function with that obtained by the Smirnov psychophysical method for the same eyes. Wave-front profiles measured by the two methods agreed closely in terms of shape and magnitude with rms differences of approximately lambda/2 and approximately lambda/6 (5.6-mm pupil) for two eyes. Primary spherical aberration was dominant in these profiles, and, in one subject, secondary coma was opposite in sign to primary coma, thereby canceling its effect. Discovery of an unusual, subtle wave-front anomaly in one individual further demonstrated the accuracy and sensitivity of the Shack-Hartmann wave-front sensor for measuring the optical quality of the human eye.     

Groups of algebraic integers used for coding QAM signals

Linear block codes over Gaussian integers and Eisenstein integers were used for coding over two-dimensional signal space. A group of Gaussian integers with 2²ⁿ elements was constructed to code quadrature amplitude modulation (QAM) signals such that a differentially coherent method can be applied to demodulate the QAM signals. This paper shows that one subgroup of the multiplicative group of units in the algebraic integer ring of any quadratic number field with unique factorization, modulo the ideal (Pⁿ), can be used to obtain a QAM signal space of 2p^2n-2 points, where p is any given odd prime number. Furthermore, one subgroup of the multiplicative group of units in the quotient ring Z[ω]/(pⁿ) can be used to obtain a QAM signal space of 6p^2n-2 points; one subgroup of the multiplicative group of units in the quotient ring Z[i](pⁿ) can be used to obtain a QAM signal space of 4p^2n-2 points which is symmetrical over the quadrants of the complex plane and useful for differentially coherent detection of QAM signals; the multiplicative group of units in the quotient ring Z[ω]/(2ⁿ) can be used to obtain a QAM signal space of 3·2^2n-2 points, where i=√-1, ω=(-1+√-3)/2=(-1+i√3)/2, p is any given odd prime number, Z[i] and Z[ω] are, respectively, the Gaussian integer ring and the Eisenstein integer ring. These multiplicative groups can also be used to construct block codes over Gaussian integers or Eisenstein integers which are able to correct some error patterns     

Observation of magneto-thermal instabilities in (YxTm1−x)Ba2Cu3O7 single crystals in pulsed magnetic field magnetization measurements

The understanding of flux jumps in the high temperature superconductors is of importance since the occurrence of these jumps may limit the perspectives of the practical use of these materials. In this work we present the experimental study of the role of heavy ion irradiation in stabilizing the HTSC against flux jumps by comparing un-irradiated and 7.5 · 10¹⁰ Kr-ion/cm² irradiated (Y_xTm_1–x)Ba₂Cu₃O₇ single crystals. Using pulsed field magnetization measurements, we have applied broad range of field sweep rates from 0.1T/s up to 1800 T/s to investigate the behavior of the flux jumps. The observed flux jumps, which may be attributed to thermal instabilities, are incomplete and have different amplitudes. The flux jumps strongly depend on the magnetic field, on the magneto-thermal history of the sample, on the magnetic field sweep rate, on the critical current density j_c, on the temperature and on the thermal contact with the bath in which the sample is immersed.     

Thermotropic phase properties of the hydroxyceramide/cholesterol system

The interaction of cholesterol with ceramides containing α-hydroxy fatty acyl chains (hydroxyceramides) has been studies as a foundation for characterizing the lipid bilayers of thestratum corneum. A relatively large quantity of cerebrosides was obtained from bovine brain and converted to ceramides through removal of the carbohydrate side chain. The ceramides were separated based on the absence or presence of hydroxy fatty acyl chains. The lyophilized hydroxyceramides showed a broad melting region at 92°C. Hydroxyceramides dispersed in water produced a relatively narrow, thermotropic transition at 75°C. The effect of cholesterol on this thermotropic phase transition of hydroxyceramides was determined by differential scanning calorimetry. With respect to the main transition, cholesterol caused a broadening of the phase transition at relatively low levels as well as a decrease in the peak transition temperature. The presence of cholesterol at levels in excess of 7 wt% gave rise to an additional low-temperature transition at 55°C. Upon immediate rescanning, this transition was exothermic, but with increasing incubation time the area under the excess heat capacity curve as a function of temperature became smaller. After two days or more, the transition observed was endothermic. At cholesterol levels between 40 and 50 wt%, multiple peaks were observed. From comparisons with related systems, the cooperative thermal transitions of hydroxyceramides with cholesterol are suggested to result from changes in hydrogen bonding or be due to phase separation. The composition of isolated brain ceramides is being compared with that reported for thestratum corneum.     

Gas-liquid mass transfer in a circulating jet-loop nitrifying MBR

Based on airlift configuration, a novel circulating jet-loop submerged membrane bioreactor (JLMBR) adapted to ammonium partial oxidation has been developed. Membrane technology and combined air and water forced circulation are adopted to obtain a high biomass retention time and to achieve a separate control of mixing and aeration. This study is intended to determine how gas-liquid mass transfer is affected by operating conditions. In a first approximation, liquid was assumed to be perfectly mixed. A classical non-steady state clean water test, known as the “gas out-gas in” method, was used to determine the gas-liquid mass transfer coefficient k_La. Air and recirculated liquid superficial velocities were gradually increased from 0.013 to and 0.0056 to , respectively. Subsequently, the gas-liquid mass transfer coefficient k_La varied from 0.01 to . It appears to be influenced by the combined action of air and recirculated liquid flowrates in the range and , respectively, for air and liquid. Correlations are proposed to describe this double influence. Experiments were performed on tap water and a culture medium used for the autotrophic growth of nitrifying bacteria, respectively. Oxygen transfer appeared to be not significantly affected by the mineral salt encountered in this medium.     

Electroosmotic control of width and position of liquid streams in hydrodynamic focusing

This paper presents theoretical and experimental investigations on electroosmotic control of stream width in hydrodynamic focusing. In the experiments, three liquids (aqueous NaCl, aqueous glycerol and aqueous NaCl) are introduced by syringe pumps to flow side by side in a straight rectangular microchannel. External electric fields are applied on the two aqueous NaCl streams. Under the same inlet volumetric flow rates, the applied electric fields are varied to control the interface positions and consequently the width of the focused aqueous glycerol stream. The electroosmotic effect on the width of the aqueous glycerol is measured using fluorescence imaging technique. The electroosmotic effect under different flow rates, different viscosity, and aspect ratio are investigated. The results indicate that the electroosmotic effect on the pressure-driven flow becomes weaker with the increase in flow rates, viscosity ratio or aspect ratio of the channel. The measured results of the focused width of the non-conducting fluid agree well with the analytical model.     

Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy

Kidney failure is a major health problem worldwide. Patients with end-stage renal disease require intensive medical support by dialysis or kidney transplantation. Current methods for diagnosis of kidney disease are either invasive or insensitive, and renal function may decline by as much as 50% before it can be detected using current techniques. The goal of this study was, therefore, to identify biomarkers of kidney disease (associated with renal fibrosis) that can be used for the development of a non-invasive clinical test for early disease detection. We utilized two protein-profiling technologies (SELDI-TOF MS and 2-D) to screen the plasma and kidney proteome for aberrantly expressed proteins in an experimental mouse model of unilateral uretric obstruction, which mimics the pathology of human renal disease. Several differentially regulated proteins were detected at the plasma level of day-3-obstructed animals, which included serum amyloid A1, fibrinogen α, haptoglobin precursor protein, haptoglobin and major urinary proteins 11 and 8. Differentially expressed proteins detected at the tissue level included ras-like activator protein 2, haptoglobin precursor protein, malate dehydrogenase, α enolase and murine urinary protein (all p<0.05 versus controls). Immunohistochemistry was used to confirm the up-regulation of fibrinogen. Interestingly, these proteins are largely separated into four major classes: (i) acute-phase reactants (ii) cell-signaling molecules (iii) molecules involved in cell growth and metabolism and (iv) urinary proteins. These results provide new insights into the pathology of obstructive nephropathy and may facilitate the development of specific assay(s) to detect and monitor renal fibrosis.     

Quantitative Proteomics Study Reveals Changes in the Molecular Landscape of Human Embryonic Stem Cells with Impaired Stem Cell Differentiation upon Exposure to Titanium Dioxide Nanoparticles

The increasing number of nanoparticles (NPs) being used in various industries has led to growing concerns of potential hazards that NP exposure can incur on human health. However, its global effects on humans and the underlying mechanisms are not systemically studied. Human embryonic stem cells (hESCs), with the ability to differentiate to any cell types, provide a unique system to assess cellular, developmental, and functional toxicity in vitro within a single system highly relevant to human physiology. Here, the quantitative proteomics approach is adopted to evaluate the molecular consequences of titanium dioxide NPs (TiO₂ NPs) exposure in hESCs. The study identifies ≈328 unique proteins significantly affected by TiO₂ NPs exposure. Proteomics analysis highlights that TiO₂ NPs can induce DNA damage, elevated oxidative stress, apoptotic responses, and cellular differentiation. Furthermore, in vivo analysis demonstrates remarkable reduction in the ability of hESCs in teratoma formation after TiO₂ NPs exposure, suggesting impaired pluripotency. Subsequently, it is found that TiO₂ NPs can disrupt hESC mesoderm differentiation into cardiomyocytes. The study unveils comprehensive changes in the molecular landscape of hESCs by TiO₂ NPs and identifies the impact which TiO₂ NPs can have on the pluripotency and differentiation properties of human stem cells.