Improving basin water use in linked agricultural, ecological and urban systems: seeking new flow paths and avoiding dead ends.

In many river basins, patterns of water withdrawals, deliveries, depletion, return flows, and reuse are prevalent. These cascading systems, often misunderstood, provide opportunities, but also threats to livelihoods and environment. Different ways of thinking about these cascading systems are required.     

Estimation of Fourier coefficients

It is shown that the maximum-likelihood estimation or robust estimation of the Fourier coefficients may be preferable to Fourier transformation if the noise contains outliers or is otherwise not normally distributed. The reason is that, in that case, these estimators produce Fourier coefficient estimates and, therefore, system parameter estimates having a smaller variance     

Procol

PROCOL is an object-oriented language with distributed delegation. It strongly supports concurrency: many objects may be active simultaneously, they execute in parallel unless engaged in communication. An object has exported operations, called Actions. Only one Action can be active at a time, however special interrupt Actions may interrupt regular Actions. Communication is performed via remote procedure call, or via a one-way synchronous message with short-time binding. In communications both client and server can be specified, either by object instance identifiers, or by type. Therefore client-server mappings may be 1-1,n-1, or 1-n, though only 1 message is transferred. PROCOL controls object access by an explicit per-object protocol. This protocol is a specification of the legality and serialization of the interaction between the object and its clients. It also provides for client type checking. The use of protocols in object communication fosters structured, safer and potentially verifiable information exchange between objects. The protocol also plays an important role as a partial interface specification. In addition it acts as a composition rule over client objects, representing relations with the client objects. PROCOL's communication binding is dynamic (run-time); it functions therefore naturally in a distributed, incremental and dynamic object environment. PROCOL also supports constraints, without compromising information hiding. An implementation is available in the form of aC extension.This author is supported by the Dutch Science Organization NWO     

Energy optimization for CAN bus and media controls in electric vehicles using deep learning algorithms

The Journal of Supercomputing - This study offers a neural network-based deep learning method for energy optimization modeling in electric vehicles (EV). The pre-processed driving cycle is...     

The Ras/Erk pathway induces primitive endoderm but prevents parietal endoderm differentiation of F9 embryonal carcinoma cells

The formation of parietal endoderm (PE) is one of the first differentiation processes during mouse development and can be studied in vitro using F9 embryonal carcinoma (EC) cells. Treatment of F9 EC cells with retinoic acid (RA) induces differentiation toward primitive endoderm (PrE), while differentiation toward PE is induced by subsequent addition of parathyroid hormone (PTH) or PTH-related peptide (PTHrP). The signal transduction mechanisms involved in this two-step process are largely unclear. We show that the RA-induced differentiation toward PrE is accompanied by a sustained increase in Ras activity and that ectopic expression of oncogenic Ha-Ras is sufficient to induce PrE differentiation. Ras activity subsequently decreases upon PTH-induced differentiation toward PE. This is a necessary event, since expression of oncogenic Ha-Ras in PrE-like cells prevents PTH-induced PE differentiation. Expression of active PKA in PrE-like F9 cells mimics PTH-induced PE differentiation and is again prevented by oncogenic Ha-Ras. The effect of oncogenic Ras on both differentiation steps is abolished by the MEK inhibitor PD98059 and can be mimicked by constitutively active forms of Raf and MEK. In conclusion, our data suggest that activation of the Ras/Erk is sufficient to induce differentiation to PrE and to prevent subsequent differentiation toward PE. Activation of PKA down-regulates Ras activity, resulting in disappearance of this blockade and transmission of signal(s) triggering PE differentiation.     

Uncertainty management: The influence of uncertainty salience on reactions to perceived procedural fairness.

On the basis of fairness heuristic theory, it is argued in this article that people especially need fairness when they are reminded about aspects of their lives that make them uncertain. It is therefore proposed that thinking about uncertainty should make fairness a more important issue to people. The findings of 3 experiments support this line of reasoning: Asking (vs. not asking) participants 2 questions that solicited their thoughts and feelings of being uncertain led to stronger effects of perceived procedural fairness on participants' affective reactions toward the way they were treated. It is argued that these findings suggest that fairness matters to people especially when they are trying to deal with things that make them uncertain. An implication of the current findings therefore may be that fairness is important to people because it gives them an opportunity to manage uncertain aspects of their lives. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Changes in the signalling status of the small GTP-binding proteins Rac and Rho do not influence insulin-stimulated hexose transport

Post-receptor signalling molecules that convey the signal from the activated insulin receptor to the actual process of Glut4 translocation and hexose uptake are poorly understood. Various studies have suggested a requirement of the lipid kinase phosphatidylinositol-3 kinase (PI3-kinase) in this process. PI3kinase regulates the activation status of the small GTP-binding protein Rac which, in turn, is able to activate another G-protein Rho. Rac and Rho are known to regulate the structure of the membrane- and cytoplasmic actin-cytoskeleton. We have examined whether Rac and Rho transfer the signals generated by PI3kinase towards insulin-stimulated hexose uptake. For that purpose, we expressed in 3T3-L1 adipocytes the dominant-negative mutant of RacN17 using vaccinia virus-mediated gene transfer. The expression levels of the RacN17 protein were monitored by Western blotting. The abrogation of endogenous Rac signalling by expression of RacN17 was inferred from the observed loss of arachidonic acid release in response to insulin. Basal and insulin-stimulated hexose transport were not affected by expression of the RacN17 mutant. A possible contribution of Rho.GTP to stimulation of hexose uptake was examined by pre-incubation of adipocytes with lysophosphatidic acid (LPA). We observed a profound effect of LPA on the structure of the cytoskeleton and on the phosphorylation of Focal Adhesion Kinase (p125FAK), indicating that 3T3-L1 adipocytes respond to LPA and that Rho was activated by LPA. However, no effect was detected on the basal or on the insulin-stimulated hexose transport. We conclude that Rac and Rho are unlikely to be involved in insulin-stimulated hexose transport, suggesting a possible contribution of other signalling pathways, downstream of PI3kinase to this process.     

Time-series analysis if data are randomly missing

Maximum-likelihood (ML) theory presents an elegant asymptotic solution for the estimation of the parameters of time-series models. Unfortunately, the performance of ML algorithms in finite samples is often disappointing, especially in missing-data problems. The likelihood function is symmetric with respect to the unit circle for the estimated zeros of time-series models. As a consequence, the unit circle is either a local maximum or a local minimum in the likelihood of moving-average (MA) models. This is a trap for nonlinear optimization algorithms that often converge to poor models, with estimated zeros precisely on the unit circle. With ML estimation, it is much easier to estimate a long autoregressive (AR) model with only poles. The parameters of that long AR model can then be used to estimate MA and autoregressive moving-average (ARMA) models for different model orders. The accuracy of the estimated AR, MA, and ARMA spectra is very good. The robustness is excellent as long as the AR order is less than 10 or 15. For still-higher AR orders until about 60, the possible convergence to a useful model will depend on the missing fraction and on the specific properties of the data at hand.     

Acute disruption of the posterior tibial tendon associated with open-fracture dislocation of the ankle

A 27-year-old man with a high-energy, open-fracture dislocation of the ankle underwent debridement and open reduction of his injuries. During surgery a disruption-laceration of the posterior tibial tendon at the level of the fracture of the medial malleolus was found. Repair required location of the proximal muscle and tendon and controlled traction of the musculotendinous unit from its retracted position. Common aspects in the nine previously reported instances of this lesion include relatively high energy of the injury and the fracture type, a transverse fracture in the medial malleolus. Unique to this case was the open injury.