Breast-feeding and the nutritional status of nursing children in Chile

4-Pentafluoroethylumbelliferyl-beta-D-glucoside is proposed as an efficient substrate for human leukocyte acid beta-glucosidase. Its synthesis is described. This substrate was compared directly with 4-trifluoromethylumbelliferyl-beta-D-glucoside synthesized by us earlier and with 4-methylumbelliferyl-beta-D-glucoside which is commonly used for acid beta-glucosidase activity assay. The specific activity of acid beta-glucosidase with 4-pentafluoroethylumbelliferyl-beta-D-glucoside was 3- and 8-fold higher than it was with the substrates mentioned above. The kinetic parameters KM and VMAX for human leukocyte acid beta-glucosidase with the three substrates was determined. One possible application of the newly synthesized substrate is its use in the diagnosis of acid beta-glucosidase hereditary deficiency (Gaucher's disease).     

Theoretical analysis of neutron scattering spectra of4He films on graphite

We compare microscopic calculations of the dynamic structure function of helium films adsorbed to a graphite substrate with neutron scattering data. Starting from a generalized Feynman theory of collective excitations, we include successively, three-phonon interactions, self-energy corrections, and experimental broadening and thereby improve the agreement between theoretical predictions and experiments. The inclusion of three-phonon vertices allows high-lying excited states to decay into lesser energetic ones and thus leads to a natural linewidth. It is shown that the theoretical linewidth of the excitations is typically smaller than the experimental resolution, and that much structure of the spectrum in the neutron scattering data is obscured under a broad plateau between the ripplon and the phonon excitations. This analysis leaves little doubt of the existence of layer-phonons and that the observed shoulder in the dynamic structure function, near the roton minimum, is actually a 2D roton propagating in the inner-most liquid layer.     

Contour path FDTD method for analysis of pyramidal horns withcomposite inner E-plane walls

The contour path finite-difference time-domain (FDTD) method is used for modeling pyramidal horn antennas with or without composite E-plane inner walls. To model the pyramidal horn surface, a locally distorted grid is used. Modified equations are obtained based on the locally distorted grid and the assumptions of the contour path method. The developed algorithm is validated by comparing computed antenna gain patterns, with and without the presence of composite material, with available measurements     

Multiresolution segmentation of respiratory electromyographicsignals

Analysis of respiratory electromyographic (EMG) signals in the study of respiratory control requires the detection of burst activity from background (signal segmentation), and focuses upon the determination of onset and cessation points of the burst activity (boundary estimation). The authors describe a new automated multiresolution technique for signal segmentation and boundary estimation. During signal segmentation, a new transitional segment is defined which contains the boundary between background a burst activity. Boundary estimation is then performed within this transitional segment. Boundary candidates are selected and a probability is attributed to each candidate, using an artificial neural network. The final boundary for a given transitional segment is the boundary estimate with the maximum a posteriori probability. This new method has proved accurate when compared to boundaries chosen by two investigators     

Conformation and activity of mannose- and N-acetylgalactosamine-specific lectins from Vicia villosa seeds

The conformation of two Vicia villosa lectins specific for mannose and N-acetylgalactosamine, respectively, was studied by circular dichroism. Both showed a broad negative CD band around 220 nm and a positive one above 190 nm. CD data analysis indicated that they were rich in beta-sheet. However, they differed in conformational stability against extreme pH, at elevated temperature, and in guanidine hydrochloride and sodium dodecyl sulfate solutions. The unusual feature was that the conformation of N-acetylgalactosamine-specific lectin was virtually unaltered in 6 M guanidine hydrochloride and 7.5 mM surfactant.     

Morphological and kinetics study of PEO/PVC blends

The kinetics of spherulite radial growth and the morphology of the compatible system PEO/PVC have been studied by optical microscopy. The usual spherulite radial growth behaviour has been found for compatible blends with PEO content higher than 70%. For lower composition, distortion of the usual spherulite morphology has been observed; the anomalies have been attributed to partial miscibility of the components.