Repetitive Sequence Barcode Probe for Karyotype Analysis in Tripidium arundinaceum

The barcode probe is a convenient and efficient tool for molecular cytogenetics. Tripidium arundinaceum, as a polyploid wild allied genus of Saccharum, is a useful genetic resource that confers biotic and abiotic stress resistance for sugarcane breeding. Unfortunately, the basic cytogenetic information is still unclear due to the complex genome. We constructed the Cot-20 library for screening moderately and highly repetitive sequences from T. arundinaceum, and the chromosomal distribution of these repetitive sequences was explored. We used the barcode of repetitive sequence probes to distinguish the ten chromosome types of T. arundinaceum by fluorescence in situ hybridization (FISH) with Ea-0907, Ea-0098, and 45S rDNA. Furthermore, the distinction among homology chromosomes based on repetitive sequences was constructed in T. arundinaceum by the repeated FISH using the barcode probes including Ea-0663, Ea-0267, EaCent, 5S rDNA, Ea-0265, Ea-0070, and 45S rDNA. We combined these probes to distinguish 37 different chromosome types, suggesting that the repetitive sequences may have different distributions on homologous chromosomes of T. arundinaceum. In summary, this method provide a basis for the development of similar applications for cytogenetic analysis in other species.     

Customized Utilization Strategies of Industrial Lignin to Produce Adsorbents and Flocculants Based on Fractionation and Adequate Structural Interpretation

Lignin, a by-product of pulping and biorefinery, has great potential to replace petrochemical resources for wastewater purification. However, the defects of lignin, such as severe heterogeneity, inferior reactivity and poor solubility, characterize the production process of lignin-based products by high energy consumption and serious pollution. In this study, several lignin fractions with relatively homogeneous structure were first obtained by organic solvent fractionation, and their structures were fully deciphered by various characterization techniques. Subsequently, each lignin component was custom-valued for wastewater purification based on their structural characteristics. Benefiting from the high reactivity and reaction accessibility, the lignin fraction (lignin-1) refined by dissolving in ethanol and n-butanol could been used as a raw material to produce cationic lignin-based flocculant (LBF) in a copolymerization system using green, cheap and recyclable ethanol as solvent. The lignin fraction (lignin-2) extracted by methanol and dioxane showed low reactivity and high carbon content, which was used to produce lignin-based activated carbon (LAC) with phosphoric acid as activator. Moreover, the influences of synthetic factors on the purification capacity were discussed, and the LBF and LAC produced under the optimal conditions showed distinguished purification effect on kaolin suspension and heavy metal wastewater, respectively. Furthermore, the corresponding purification mechanism and external factors were also elaborated. It is believed that this cleaner production strategy is helpful for the valorization of lignin in wastewater resources.     

Strengthening and toughening mechanism of a Cu-bearing high-strength low-alloy steel with refined tempered martensite/bainite (M/B) matrix and minor inter-critical ferrite

The microstructure–mechanical property relationship of a Cu-bearing low-carbon high-strength low-alloy steel, subjected to a novel multistage heat treatment inc...     

光子晶体光纤飞秒激光技术研究进展

光子晶体光纤自诞生至今的十几年来得到了快速发展,不同结构和各具特色的光子晶体光纤层出不穷。以应用于飞秒激光技术的各种光子晶体光纤为主线,介绍了目前基于光子晶体光纤飞秒激光技术的实验研究进展,尤其是高功率、高能量飞秒激光系统的研究现状和发展方向。     

Linear Study of a Large-orbit Coaxial-waveguide Cyclotron Autoresonance Maser Amplifier

A linear theory based on Maxwell-Vlasov equations and the Laplace transform is presented to describe the large-orbit coaxial-waveguide cyclotron autoresonance maser (CARM) amplifier. A 35-GHz, TE_5,1-mode large-orbit coaxial-waveguide CARM amplifier is specifically analyzed. Good agreement between the linear theory and the nonlinear simulations is found in the exponentially growing region of the wave. Results show that the operating magnetic field and the electron-beam energy and average radius substantially affect the power, and especially, a small beam current may be unsuitable to a large-orbit CARM amplifier operation.     

High-Q microresonators on 4H-silicon-carbide-oninsulator platform for nonlinear photonics

The realization of high-quality (Q) resonators regardless of the underpinning material platforms has been a ceaseless pursuit, because the high-Q resonators pro...     

Dendrite?Free and Stable Lithium Metal Battery Achieved by a Model of Stepwise Lithium Deposition and Stripping

The uncontrolled formation of lithium (Li) dendrites and the unnecessary consumption of electrolyte during the Li plating/stripping process have been major obst...     

麦秸秆加筋盐渍土的尺寸效应与制样问题处置

制备均匀且整体性较好的试样是保证室内实验结果可靠的前提条件。提出了两端压实的静力制样法,围绕制样中出现的几个问题,制定了适宜的应对措施。同时完成了不同直径的盐渍土和麦秸秆加筋土试样的抗压强度实验,分析了试样抗压强度的尺寸效应,以及试样直径与加筋条件间的比例关系。结果显示:采用两端压实的静力制样方法制备加筋土试样是可行的;两种试样均存在明显的尺寸效应;由于麦秸秆的加筋作用,使得麦秸秆加筋土不同尺寸试样的抗压强度尺寸效应大于盐渍土的,且其抗压强度的尺寸效应随试样尺寸的增大而越来越明显;适宜加筋长度为0.316倍试样直径,适宜加筋率0.25%。研究结果可为制备合格的加筋土试样提供参考,也有益于在未来的研究中选择更合理的麦秸秆加筋条件。     

电流互感器密封失效引起的绝缘故障

首先介绍了油浸式电流互感器和SF₆电流互感器的整体绝缘结构,以近期多地出现的电流互感器绝缘故障为切入点,分析了电流互感器绝缘击穿的原因。通过电流互感器的解体检查进一步推断出设备故障的根源为互感器密封失效,针对油浸式电流互感器的金属膨胀器渗漏、SF₆电流互感器爆破片破损引发的密封失效现象,提出了运行维护建议和预防措施。     

Zn‐MOF‐74 Derived N‐Doped Mesoporous Carbon as pH‐Universal Electrocatalyst for Oxygen Reduction Reaction

It is of increasing importance to explore new low‐cost and high‐activity electrocatalysts for oxygen reduction reaction (ORR), which have had a substantial impact across a diverse range of energy conversion system, including various fuel cell and metal–air batteries. Although engineering carbon nanostructures have been widely explored as a candidate class of Pt‐based ORR electrocatalysts owing to their proved high activity, outstanding stability, and ease of use, there still remains a daunting challenge to develop high activity metal‐free electrocatalysts in pH‐universal electrolyte system. Here, a reliable and controllable route amenable to prepare nitrogen‐doped porous carbon (NPC) with high yields and exceptional quality is described. The as‐prepared NPC shows advantages of high activity, high durability, and methanol‐tolerant as an efficient pH‐universal electrocatalyst for ORR, showing comparable or even better activity as compared with the commercial Pt/C catalysts not only in alkaline media but also in acidic and neutral electrolyte. Systematic electrochemical studies, combining with density functional theory calculation, demonstrate the unique nitrogen‐doping species and favorable pores in the as‐designed NPC synergistically contribute to the significantly improved catalytic activity in pH‐universal medium. The present work potentially presents an important breakthrough in developing ORR electrocatalysts for various fuel cells.