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131.
Steinle ED Mitchell DT Wirtz M Lee SB Young VY Martin CR 《Analytical chemistry》2002,74(10):2416-2422
This paper describes synthetic micropore and nanotube membranes that mimic the function of a ligand-gated ion channel; that is, these membranes can be switched from an "off" state (no or low ion current through the membrane) to an "on" state (higher ion current) in response to the presence of a chemical stimulus. Ion channel mimics based on both microporous alumina and Au nanotube membranes were investigated. The off state was obtained by making the membranes hydrophobic by chemisorbing either a C18 silane (alumina membrane) or a C18 thiol (Au nanotube membrane). Water and electrolyte are forbidden from entering these very hydrophobic pores/nanotubes. The transition to the on state was induced by the partitioning of a hydrophobic ionic species (e.g., a drug or a surfactant) into the membrane. The membrane switches to the on state because at a sufficiently high concentration of this ionic analyte species, the pores/nanotubes flood with water and electrolyte. A pH-responsive membrane was also prepared by attaching a hydrophobic alkyl carboxylic acid silane to the alumina membrane. 相似文献
132.
S. J. Bull R. I. Davidson K. Harvathova D. Mitchell J. R. White 《Journal of Materials Science》2002,37(22):4937-4943
Controlled scratch testing has been applied to experimental coatings of the kind used on marine structures, based on epoxy-amine resins. Measurements of the scratch hardness and the critical load to failure have been made. Inspection of the scratch tracks by reflected light microscopy and scanning electron microscopy enabled identification of the failure mechanism. Failure could be by though-thickness cracking or adhesion failure. The failure mechanism is sensitive to the coating thickness and detachment from the substrate was obtained only from coatings less than 300 m thick in the as-prepared state for tests conducted with a limiting load of 80 N. The deterioration of properties of samples immersed in hot seawater was very sensitive to composition. 相似文献
133.
Timothy J. F. Mitchell Sherry Y. Chen & Robert D. Macredie 《Journal of Computer Assisted Learning》2005,21(1):53-64
Abstract Prior knowledge is often argued to be an important determinant in hypermedia learning, and may be thought of as including two important elements: domain expertise and system expertise. However, there has been a lack of research considering these issues together. In an attempt to address this shortcoming, this paper presents a study that examines how domain expertise and system expertise influence students' learning performance in, and perceptions of, a hypermedia system. The results indicate that participants with lower domain knowledge show a greater improvement in their learning performance than those with higher domain knowledge. Furthermore, those who enjoy using the Web more are likely to have positive perceptions of non-linear interaction. Discussions on how to accommodate the different needs of students with varying levels of prior knowledge are provided based on the results. 相似文献
134.
Let X and Y be two run-length encoded strings, of encoded lengths k and l, respectively. We present a simple O(|X|l+|Y|k) time algorithm that computes their edit distance. 相似文献
135.
136.
P. Peralta A. Czapka K. Obergfell L. Llanes C. Laird T. E. Mitchell 《Materials Science and Engineering: A》1999,270(2):32
Fatigue tests were performed on pure copper polycrystals with a crystallographic texture different from that produced by ‘standard’ thermomechanical treatments, which emphasize multi-slip 111–100 textures. The texture along the loading axis deviated by 10–15° from these two poles for the samples used here. The experiments were initiated by ramp loading as a mechanical pretreatment and the cyclic stress–strain curve (CSSC) was established by step tests using enough cycles at each step to insure saturation. Under these conditions, a plateau was observed in the CSSC at an appropriate stress level and in a reproducible fashion. 相似文献
137.
Gahagan KT Gopalan V Robinson JM Jia QX Mitchell TE Kawas MJ Schlesinger TE Stancil DD 《Applied optics》1999,38(7):1186-1190
We report what we believe to be the first stand-alone integrated electro-optic lens and scanner fabricated on a single crystal of Z-cut LiTaO(3). The independently controlled lens and scanner components consist of lithographically defined domain-inverted regions extending through the thickness of the crystal. A lens power of 0.233 cm(-1) kV(-1) and a deflection angle of 12.68 mrad kV(-1) were observed at the output of the device. 相似文献
138.
Describes a method for generating a one-stage, one-sided switching network with the minimum number of switches for any required level of connectivity. The fact that the method generates optimal networks is established by proving appropriate lower bounds. This generalises recent work of Newbury 相似文献
139.
140.
H Okada J Woodcock-Mitchell J Mitchell T Sakamoto K Marutsuka BE Sobel S Fujii 《Canadian Metallurgical Quarterly》1998,97(21):2175-2182
BACKGROUND: Ischemia with or without reperfusion induces the release of diverse products from monocytes, including cytokines such as interleukin-1 (IL-1). To determine whether these phenomena modulate fibrinolysis and potentially exacerbate impairment of the macrocirculation, microcirculation, or both, we characterized the effects of IL-1 on the expression of fibrinolytic system and matrix proteins in rat cardiac microvascular endothelial cells (CMECs). METHODS AND RESULTS: Confluent CMECs were exposed to IL-1 in serum-free medium for 24 hours, and cell-conditioned medium was assayed for plasminogen activator inhibitor type 1 (PAI-1), the primary physiological inhibitor of plasminogen activators, and for type 1 collagen with Western blotting. IL-1 (2 ng/mL) specifically increased the accumulation of PAI-1 (4.4 +/- 0.6-fold; mean +/- SD; n = 9) without affecting tissue plasminogen activator (t-PA) or urokinase plasminogen activator (u-PA) levels, which remained unchanged. IL-1 increased the accumulation of collagen in conditioned media by 3.5 +/- 0.7-fold (n = 6). Conversely, the accumulation of both PAI-1 and collagen induced by IL-1 was inhibited with an IL-1 receptor antagonist (200 ng/mL; n = 6) and with cycloheximide (10 micrograms/mL; n = 6), implying that protein synthesis was a requirement for the effect. To determine whether the IL-1 effect was mediated by induction of oxygen-centered free radical production, known to be induced by IL-1, we exposed the cells to the hydroxyl radical scavenger tetramethylthiourea (10 mmol/L) and observed abolition of the IL-1-induced increase in the expression of PAI-1 and collagen (n = 6). Conversely, superoxides (generated with 10 mU/mL xanthine oxidase plus 0.6 mmol/L hypoxanthine, and 100 mumol/L hydrogen peroxide) induced the accumulation of PAI-1 and collagen (n = 6). IL-1 (1 microgram/kg body wt) and lipopolysaccharide (50 micrograms/kg body wt) administered in vivo increased PAI-1 protein in rat hearts as detected with Western blotting and PAI-1 immunostaining of rat heart microvessels, indicating the effects delineated in vitro were paralleled by effects in vivo. CONCLUSIONS: These results indicate that IL-1-induced oxygen-centered free radicals stimulate elaboration of PAI-1 and collagen by CMECs. Accordingly, microvascularly mediated inhibition of fibrinolysis may predispose to the persistence of microvascular thrombi, thereby contributing to impaired microcirculatory function, the no-reflow phenomenon, and cardiac dysfunction after ischemia and reperfusion. 相似文献