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231.
    
Irreversible electroporation (IRE) is an innovative cell ablation method based on the concept that the application of excessive electric pulses induces a lethal increase in the permeability of the cell membrane owing to nanoscale defects, resulting in a gentle form of necrotic cell death. Although the mechanism of cell death by IRE is primarily nonthermal, thermal effects are inevitable because electric pulses inherently generate Joule heat. The larger the applied voltage to treat a large target, the greater the Joule heating and the consequent temperature rise. Therefore, the temperature increase due to Joule heating during pulse application should be carefully controlled to minimize thermal damage. Research on IRE is an interdisciplinary endeavor incorporating health science for humanitarian relief and engineering. Therefore, this study provides a comprehensive review of the thermal aspects of IRE based on existing in vitro and in vivo experimental and numerical studies. The paper begins with an overview of IRE treatment covering the geometry and arrangement of electrodes, pulse parameters, and cell death mechanisms, followed by sections on thermal damage evaluation that summarize the significant work of experiments, analysis, and comparisons. Finally, thermal mitigation strategies, including electrode modification, lowering the IRE threshold, and modified pulsing protocols, are discussed.  相似文献   
232.
    
The size of cellulose microfibril (CMF) bundles varies to interact with glucomannan/galactoglucomannan (GM/GGM). Arabino-4-O-methylglucuronoxylan (AGX) bonded CMF bundles coated with GM/GGM also have important roles in elaborating the distance between these components. Since the precise roles of GM/GGM and AGX are not clear, the elution analysis to evaluate the strength of the interaction between the cell wall were tried. Earlywood (EW) and latewood (LW) were separated in a Japanese cedar. The chemical components of cellulose, hemicellulose including GM/GGM and AGX, and lignin were almost the same in EW and LW. Slight differences in GM/GGM, the side-chain substitution in AGX and the ionic bond characteristics of glucuronic acid side chains were observed. Based on measurements of GM/GGM and AGX adhering to CMFs, there were more hemicelluloses forming strong hydrogen bonds in LW than in EW. The results showed that the highly assembled hemicellulose in LW produced a strong cell wall framework.  相似文献   
233.
    
Calcium homeostasis endoplasmic reticulum protein (CHERP) is colocalized with the inositol 1,4,5-trisphosphate receptor (IP3R) in the endoplasmic reticulum or perinuclear region, and has been involved in intracellular calcium signaling. Structurally, CHERP carries the nuclear localization signal and arginine/serine-dipeptide repeats, like domain, and interacts with the spliceosome. However, the exact function of CHERP in the nucleus remains unknown. Here, we showed that poly(A)+ RNAs accumulated in the nucleus of CHERP-depleted U2OS cells. Our global analysis revealed that CHERP regulated alternative mRNA splicing events by interaction with U2 small nuclear ribonucleoproteins (U2 snRNPs) and U2 snRNP-related proteins. Among the five alternative splicing patterns analyzed, intron retention was the most frequently observed event. This was in accordance with the accumulation of poly(A)+ RNAs in the nucleus. Furthermore, intron retention and cassette exon choices were influenced by the strength of the 5′ or 3′ splice site, the branch point site, GC content, and intron length. In addition, CHERP depletion induced anomalies in the cell cycle progression into the M phase, and abnormal cell division. These results suggested that CHERP is involved in the regulation of alternative splicing.  相似文献   
234.
    
MYC is a major oncogene that plays an important role in cell proliferation in human cancers. Therefore, the mechanism behind MYC regulation is a viable therapeutic target for the treatment of cancer. Comprehensive and efficient screening of MYC regulators is needed, and we had previously established a promoter screening system using fluorescent proteins and the CRISPR library. For the efficient identification of candidate genes, a database was used, for which mRNA expression was correlated with MYC using datasets featuring “Similar” and “Not exactly similar” contexts. INTS14 and ERI2 were identified using datasets featuring the “Similar” context group, and INTS14 and ERI2 were capable of enhancing MYC promoter activity. In further database analysis of human cancers, a higher expression of MYC mRNA was observed in the INTS14 mRNA high-expressing prostate and liver cancers. The knockdown of INTS14 in prostate cell lines resulted in decreased MYC mRNA and protein expression and also induced G0/1 arrest. This study confirmed that CRISPR screening combined with context-matched database screening is effective in identifying genes that regulate the MYC promoter. This method can be applied to other genes and is expected to be useful in identifying the regulators of other proto-oncogenes.  相似文献   
235.
In this paper we propose a new learning rule for a spatiotemporal neural network model of the primate saccadic system with a distributed feedback control mechanism. In our model the superior colliculus is represented as the distributed network model and it provides a dynamic control signal to a lumped brain stem model (Robinson–Gisbergen model). Distributed feedforward and feedback weights between the deeper layer of the superior colliculus model and the brain stem model are trained using a finite time interval learning rule based on a steepest descent method. Simulations are carried out on a 20-cell model for horizontal saccades using eye position feedback and velocity feedback, respectively. The model makes accurate saccades to all target locations over the range 2 to 15 degrees even if disturbance is added to the burst generator in the brain stem model. © 1999 Scripta Technica, Electr Eng Jpn, 129(4): 66–76, 1999.  相似文献   
236.
The activity of nickel molybdenum phosphide catalysts was studied for the hydrodesulfurization of dibenzothiophene at 573 K and total pressure of 2.0 MPa. The Al2O3-supported NiMo phosphide catalysts were prepared by successive and simultaneous methods. The effect of the reduction temperature on the catalyst activity was also studied. The simultaneous preparation was determined to be the best method for the preparation of the active supported catalyst for dibenzothiophene HDS. The 623 K-reduced catalyst had the highest HDS rate of the catalysts. Nickel migrated from the inside to the surface during the reaction and promoted the HDS activity. The active species in the dibenzothiophene HDS and the oxidation states of Mo, Ni and P in the catalyst before and after reaction and of S after the reaction were studied on the basis of an XPS analysis.  相似文献   
237.
    
The characterization of aortic valve interstitial cells (VICs) cultured under optimal conditions is essential for understanding the molecular mechanisms underlying aortic valve stenosis. Here, we propose 2% hypoxia as an optimum VIC culture condition. Leaflets harvested from patients with aortic valve regurgitation were digested using collagenase and VICs were cultured under the 2% hypoxic condition. A significant increase in VIC growth was observed in 2% hypoxia (hypo-VICs), compared to normoxia (normo-VICs). RNA-sequencing revealed that downregulation of oxidative stress-marker genes (such as superoxide dismutase) and upregulation of cell cycle accelerators (such as cyclins) occurred in hypo-VICs. Accumulation of reactive oxygen species was observed in normo-VICs, indicating that low oxygen tension can avoid oxidative stress with cell-cycle arrest. Further mRNA quantifications revealed significant upregulation of several mesenchymal and hematopoietic progenitor markers, including CD34, in hypo-VICs. The stemness of hypo-VICs was confirmed using osteoblast differentiation assays, indicating that hypoxic culture is beneficial for maintaining growth and stemness, as well as for avoiding senescence via oxidative stress. The availability of hypoxic culture was also demonstrated in the molecular screening using proteomics. Therefore, hypoxic culture can be helpful for the identification of therapeutic targets and the evaluation of VIC molecular functions in vitro.  相似文献   
238.
239.
A spectrum-imaging technique based on scanning transmission electron microscopy combined with an electron energy-loss spectroscopy has been applied for the multilayer of amorphous titanium oxide and aluminium oxide layers on silicon substrate. We demonstrate the high-resolution elemental mapping and discuss the advantage of this method compared to an energy-filtering transmission electron microscopy. The main advantage is the absence of chromatic broadening, which allows the use of a large collection angle to acquire spectrum-image data and a wide energy window to integrate the core-loss signals. This suggests that the spatial resolution of elemental maps is mainly determined by the size of the electron probe.  相似文献   
240.
Vapor pressures of Bi2 (g) over La0.03 Bi0.97 and two-phase mixtures of Bi+LaBi2, LaBi2+LaBi, LaBi+La4Bi3, La4Bi3+La5Bi3, and La5Bi3+La2 Bi were measured in the temperature range from 795 to 1066K with a time-of-flight mass spectrometer equipped with a tungsten Knudsen cell. The vapor pressure of Bi2(g) over the two-phase mixtures of La2Bi+La could not be detected experimentally in this temperature range. The thermodynamic activity of bismuth was determined from the vapor pressure of Bi2(g). Thermodynamic activities of lanthanum over alloys were derived from those of bismuth in the alloys in this study and that of lanthanum in the bismuth in a previous study by graphic integration of the Gibbs–Duhem equation. Thermodynamic quantities such as Gibbs free energy of formation, excess enthalpy, etc., were also calculated from the thermodynamic activities.  相似文献   
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