Convenient colorimetric and fluorometric assays for S-nitrosothiols

S-nitrosothiols have been shown to affect a number of physiological functions. Several techniques have been used to detect these species in biological systems, primarily by methods utilizing chemiluminescence. Since the apparatus required for measurement of chemiluminescence are not readily available in most laboratories, methods employing more conventional techniques such as uv-vis and fluorescence spectroscopy may be of greater use. Herein, we report the development of colorimetric and fluorometric methods for the reliable quantitation of S-nitrosothiols. Solutions containing sulfanilamide/N-(1-naphthyl)- ethylenediamine dihydrochloride or 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid), when exposed to S-nitrosoglutathione (GSNO), S-nitrosocysteine, or S-nitrosoacteylpenicillamine, resulted in no absorbance changes in the range of 400-800 nm. Exposure to HgCl2 or Cu(acetate)2 resulted in release of nitric oxide (NO) from the S-nitrosothiols. The liberated NO reacted subsequently with oxygen and formed a chemical species which reacted with either analysis solution, resulting in an increase in absorption between 400 and 800 nm. A plot of RSNO versus absorbance was linear for both mercury(II) and copper(II) ions where the slope in the presence of mercury ion was significantly greater than that for copper ion. The sensitivity was as low as 5 microM RSNO using HgCl2. The fluorometric method using 2, 3-diaminonaphthalene as the scavenger of the NOsolidusO2 products gave a sensitivity of 50 nM for GSNO. In addition, S-nitrosylated proteins were quantitated using the fluorometric technique. These methods provide accurate determination of low concentrations of S-nitrosothiols, utilizing conventional spectroscopic techniques available in most laboratories.     

LOW TEMPERATURE FLOW CHARACTERISTICS OF SOME WAXY CRUDE OILS IN RELATION TO THEIR COMPOSITION : PART II EFFECT OF WAX COMPOSITION AND CONCENTRATION ON THE DEW AXED CRUDE OILS WITH / WITHOUT ADDITIVES

In this part of the work the effect of wax concentrations on the rheologica! properties of the dewaxed Duliajan (Assam) and Lingaia crude oils have been studied. The dewaxing of the crude oil was done by removing n-paraffins from the crude oil by urea adduction. The urea adducted n-paraffins concentrate-the wax- was characterized by its carbon number distribution. The urea non adducted oil portion - the denormalized crude oil (DNO)- was characterized for its pour point. The wax in different concentration was then added in the DNO and the change in the rheological properties like pour point, yield stress and plastic viscosity were studied using a Haake Rota Viscometer. The effect of carbon number distribution, i.e. the composition of the wax, on these rheological properties was also investigated. Four different commercial pour point depressanl additives have been used to study their effects on the pour point, yield stress and plastic viscosity of the reconstituted (wax mixed DNO) crude oils.

From this study it has been found that wax concentration and its composition are primarily responsible for the variation in the pour point of the DNO, and the DNO composition has a small effect. However, as seen in the Part I of this paper, the response of the pour point depressant additive in effecting the change in the pour point is primarily governed by the composition of the DNO. The additives have shown better response in the DNO having more aromatic concentrate, as has been found in the case of Duliajan (Assam) DNO.     

Analysis of a wireless MAC protocol with client-server traffic andcapture

We analyze an efficient medium access control (MAC) protocol for use in a single cell of a wireless local area network (LAN). A fixed frame structure with two periods is used; one period for making reservations using a slotted Aloha protocol and a second period for data transmission. The MAC protocol operates in a centralized manner in which a single station, the base station, accepts reservations (transmission or data requests) that are made by the remote stations, and then schedules the times in which they will transmit or receive data. We consider two different types of client-server traffic models, an open-loop and a closed-loop model. In the open-loop model, a remote station can generate reservation requests for the base station whether or not it has received a response from the base station. In the closed-loop case, the remote station waits for a response before generating a new request. The performance of the MAC protocol was analyzed exactly for both the open and closed-loop traffic models and for cases in which transmission errors and a mean-value type of radio capture model are included. In addition, different policies were considered for the transmission strategy that is used by the remote stations when they attempt to make reservations. We derive exact results for the mean throughput and waiting times as well as for the queue length distributions. We also derive an approximate Markov chain to treat a case in which a fixed-position capture model is used. Several types of behavior are illustrated through the use of numerical examples     

Micro-macro property correlations in alkali halide crystals

Correlations are developed between the microscopic parameters, Debye-Waller factor and lattice constant and the macroscopic properties, melting temperature and hardness of alkali halide crystals with NaCl structure. Proper equations are proposed and physical justification is provided for the observed correlations. Further, these properties are correlated with the force constant calculated from the compressibility and these correlations are, again, shown to have physical justification. Finally, a kaleidoscopic relationship is shown to exist between all these properties.     

A method for maximising support leg stress in a pressure vessel mounted on four legs subject to moment and lateral loadings

Pressure vessels mounted on four-leg type supports form a non-isotopic support system with respect to lateral loads or overturning moments. Several loadings—for example, horizontal earthquake motions and wind loads—do not have a predefined direction of action. Structural safety analysis requires the determination of the most vulnerable direction of loading for the structure. Closed-form expressions for these loadings under certain conditions are derived in this paper. The importance of ‘optimality’ with respect to the developed stress in the support is illustrated via a numerical example.     

Critical appraisal of piping phenomena in earth dams

This paper presents a comprehensive review of published literature on soil piping phenomena. The first tools to design earth dams to resist piping were developed during 1910–1935. Filter criteria for dispersive soils was refined in the 1970’s. Piping phenomena are generally defined as: (1) heave, (2) internal erosion, (3) backwards erosion, although other modes are possible. Recent work on piping highlights the limitations of the occurrence of piping and the role that design and construction may play in a large percentage of piping failures. Standardized laboratory procedures are available to assess piping potential in cohesive materials, but no such methods exist for non-cohesive soils. However, methods are available for evaluation of self-filtration potential. Recent advances in computer technology have facilitated the evaluation of seepage and deformation in embankments but computational methods for evaluation of piping potential are currently limited.      

Perinephric abscess (presenting as abdominal pain) due to Listeria monocytogenes

The development of a haematoma after wide excision of breast tumours is not uncommon. Suction drainage is commonly used to prevent this but often increases the hospital stay and can create unsightly wounds. Argon beam coagulation is a new technique which permits large 'raw' areas to be coagulated with minimal tissue damage. We believe that this technique may reduce the development of haematomas and seromas after breast surgery. We present our initial results using argon beam coagulation after wide excision of breast tumours in 80 patients without suction drainage. One patient required a single aspiration of a seroma at the site of breast lump excision and none required surgical drainage. Argon beam coagulation appears a useful adjunct in breast surgery and may help reduce hospital stay.     

Temperature rise in a viscoplastic material during dynamic crack growth

Dynamic steady-state crack growth has been analyzed under mode I plane stress, small-scale yielding conditions using a finite element procedure. A Perzyna type viscoplastic constitutive equation has been employed in this analysis. The viscoplastic work rate is converted into heat input and the temperature distribution is determined by solving the governing conduction/convection equation also by a finite element method. The Stream-line Upwinding Petrov-Galerkin formulation has been employed for this purpose because of the high Péclet number that results in such a type of analysis. The effect of strain rate sensitivity and crack speed on the temperature distribution near the crack tip is examined.