Droplet-based biochemical assay by magnetic wire manipulation between multiple droplets

Reaction performance of a droplet-based biochemical assay technique that uses magnetic wires as sample carriers is evaluated. Wires 2.0 mm in length, 0.05 mm in width, and 0.02 mm in thickness are fabricated by chemical etching, introduced into a droplet immersed in oil, and manipulated by the magnetic force of a moving magnet. Alkaline-phosphatase as an enzyme is immobilized on the wire surfaces by applying Au and self-assembled monolayer coatings, and the method’s on-chip reaction performance is evaluated. The enzymatic reaction is found to increase linearly as the number of wires and the reaction time increase. Relatively high performance reproducibility for enzymatic reactions is obtained; on average, the reaction absorbance, standard deviation, and coefficient of variance are found to be respectively 1.14, 0.103, and 9.1 %. The conductivity change in a fused droplet is used to evaluate the absolute volume of liquid transferred with the extracted wires and a value of 0.33 μl is obtained.     

Behavior of a mechanically anchored waterproofing membrane system under wind suction and uniform Pressure

A pressure and wind tunnel test was conducted to obtain the basic data for wind resistance design of a mechanically anchored waterproofing membrane system. The test specimen was a flat roof with the following dimensions: 2.4 m in width, 3 m in length and 0.29 m in height. The waterproofing material was polyvinyl chloride sheet reinforced with polyester fiber (PVC sheets). In the pressure test, because the applied pressure was equivalent to the pressure on the entire surface area of the roof, the billowing heights of the PVC sheet around the fastener had almost the same maximum values; therefore, the axial force at the fastener was also similar to the pressure induced by a compressor, and no lateral forces were measured. On the other hand, in the wind tunnel test, the strain of the PVC sheet around the fastener at windward side was larger than that of the leeward side. The lateral force was 70% of the axial force at a mean wind speed of 38.6 m/s. Therefore, it was clear that the characteristics of the mechanically anchored waterproofing membrane system in the pressure test and the wind tunnel test were different.     

Theory of electrostatics and electrokinetics of soft particles

We investigate theoretically the electrostatics and electrokinetics of a soft particle, i.e. a hard particle covered with an ion-penetrable surface layer of polyelectrolytes. The electric properties of soft particles in an electrolyte solution, which differ from those of hard particles, are essentially determined by the Donnan potential in the surface layer. In particular, the Donnan potential plays an essential role in the electrostatics and electrokinetics of soft particles. Furthermore, the concept of zeta potential, which is important in the electrokinetics of hard particles, loses its physical meaning in the electrokinetics of soft particles. In this review, we discuss the potential distribution around a soft particle, the electrostatic interaction between two soft particles, and the motion of a soft particle in an electric field.     

玻璃纤维/大麻纤维增强聚丙烯混杂复合材料的力学性能和断裂特征(英文)

目前在土木工程,建筑,汽车等领域里使用的复合材料中,玻璃纤维是用量最大的增强材料。由于保护地球环境的呼声日趋高涨,天然纤维被期待着代替源于石油而且再利用困难的玻璃纤维,成为绿色复合材料的必要材料之一。本文通过注射成型工艺制作了玻璃短纤维,大麻短纤维以及混杂型纤维增强复合材料,并在拉伸实验中应用两种不同频率的声发射技术检测了拉伸断裂特性。实验发现,随着大麻纤维的加入和混杂复合材料绿色度的增加,复合材料的拉伸弹性模量随之线性增大,而拉伸强度基本保持不变。当大麻纤维的含量超过20wt%的时候,拉伸强度开始降低。在不同频率的声发射实验中,混杂型复合材料的声发射事件的产生都比单一纤维增强型复合材料要来的晚,也就是说,纤维的混杂有助于推迟微裂纹的产生。     

Poly(p‐phenylene sulfide) nanofibers prepared by CO2 laser supersonic drawing

Poly(p‐phenylene sulfide) (PPS) nanofibers are prepared by irradiating a PPS fiber with a carbon dioxide (CO₂) laser while drawing it at supersonic speeds. A supersonic jet is generated by blowing air into a vacuum chamber through the fiber injection orifice. Nanofibers obtained at a laser power of 30 W and chamber pressure of 10 kPa exhibit an average diameter of 600 nm and a draw ratio of 110,000. Scanning electron microscopy, differential scanning calorimetry, and wide‐angle X‐ray diffraction analyses are employed to investigate the relationships among the chamber pressure, fiber morphology, and crystallization behavior. The nanofibers exhibit two melting temperatures (T_m): approximately 280°C and 320°C. The endothermic peak at T_m = 280°C is ascribable to lamellar crystals and that at T_m = 320°C to the highly complete crystals, since the polymer molecular chain is highly oriented. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40922.     

The terminal protein of the linear DNA plasmid pGKL2 shares an N-terminal domain of the plasmid-encoded DNA polymerase

The 36K protein attached at the 5′ end of the linear DNA plasmid pGKL2 from the yeast Kluyveromyces lactis was first purified and characterized. The terminal protein was purified from cells (1 kg wet weight) by ammonium sulphate precipitation and two rounds of centrifugation to equilibrium in CsCl gradients. The pGKL2 was present only in the post-microsomal supernatant. Approximately 10 mg of the purified pGKL2 was recovered and digested with DNase I. The terminal protein (final ca. 0·8 mg) was homogeneous by electrophoresis and we determined the N-terminal amino acid sequence up to ten residues, showing that it existed in the cryptic N-terminal domain of pGKL2-ORF2 (DNA polymerase) sequence.     

Nucleotide sequences of alcohol acetyltransferase genes from lager brewing yeast,Saccharomyces carlsbergensis

The nucleotide sequences of alcohol acetyltransferase genes isolated from lager brewing yeast, Saccharomyces carlsbergensis have been determined. S. carlsbergensis has one ATF1 gene and another homologous gene, the Lg-ATF1 gene. There was a high degree of homology between the amino acid sequences deduced for the ATF1 protein and the Lg-ATF1 protein (75·7%), but the N-terminal region has a relatively low degree of homology. Southern analysis and contour-clamped homogeneous electric field analysis of Saccharomyces strains suggest that the ATF1 gene is located on chromosome XV in S. cerevisiae and that the Lg-ATF1 gene might originate from the ‘non-S. cerevisiae’ genome of S. carlsbergensis, which is similar to that of S. bayanus and S. pastorianus. The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank data banks with the Accession Numbers D63449 (ATF1) and D63450 (Lg-ATF1).     

Generalized warpage parameter

A warpage index (Δψ_m) was introduced for studying warpage characteristics of a plastic part injection molded from PA66 compounded with 30 wt% glass fiber. Δψ_m is defined as Δψ_m = (Δψ_m)_max – (Δψ_m)_min, where ψ_m = ψ(θ)_max, where ψ(θ) = (ε(θ) – α(θ)ΔT)/| α(θ)ΔT|, where ε is the total strain, α is the linear thermal expansion coefficient, ΔT is temperature difference, and θ is the angle along which ε and α are calculated. Finite element analysis was used for calculating flow field in injection, fiber orientation, material anisotropy and warpage. ψ_m is calculated in each finite element, and Δψ_m is calculated in a whole finite element model. Δψ_m is a measure of the ratio of actual shrinkage to the amount of shrinkage that would occur if an element freely shrank. The characteristics of Δψ_m were studied. It has been found that warpage is null if Δψ_m = 0, but that null warpage generally does not indicate Δψ_m = 0. It is shown that Δψ_m quantitatively represents the warped and unwarped state. Δψ_m distinguishes the null warpage state with possible buckling from the null warpage state without possible buckling. It has been shown that material anisotropy is possibly described with Δψ_m, and that the cause of warpage is self-restrictive deformation in an injection molded part. It has been deduced that it is generally not possible to eliminate warpage only by controlling material properties. Δψ_m is obtainable for a plastic part with complex geometry and complex fiber orientation state, and for arbitrary materials. Applications of Δψ_m are left for future study.     

Therapeutic Targets and Emerging Treatments in Advanced Chondrosarcoma

Simple SummaryChondrosarcomas develop chemoresistance to standard anticancer drugs, making it difficult to control unresectable or metastatic chondrosarcomas. To improve the clinical outcomes of chondrosarcoma, new treatment approaches, such as molecule-targeting agents and immunotherapy, are needed. Recent research has revealed promising biomarkers and therapeutic targets for chondrosarcoma. In addition, several molecule-targeting agents have shown favorable antitumor activities in several clinical studies in patients with advanced sarcomas, including chondrosarcoma. This review summarizes recent basic studies on biomarkers and therapeutic targets and recent clinical studies on treating chondrosarcomas.AbstractDue to resistance to standard anticancer agents, it is difficult to control the disease progression in patients with metastatic or unresectable chondrosarcoma. Novel therapeutic approaches, such as molecule-targeting drugs and immunotherapy, are required to improve clinical outcomes in patients with advanced chondrosarcoma. Recent studies have suggested several promising biomarkers and therapeutic targets for chondrosarcoma, including IDH1/2 and COL2A1. Several molecule-targeting agents and immunotherapies have shown favorable antitumor activity in clinical studies in patients with advanced chondrosarcomas. This review summarizes recent basic studies on biomarkers and molecular targets and recent clinical studies on the treatment of chondrosarcomas.