Dissecting cAMP binding domain A in the RIalpha subunit of cAMP-dependent protein kinase. Distinct subsites for recognition of cAMP and the catalytic subunit

The two gene-duplicated cAMP binding domains in the regulatory subunits of cAMP dependent protein kinase are each comprised of an A helix, an eight-stranded beta-barrel, and a B and C helix (1). The A domain is required for high affinity binding to C, while the B domain regulates access to the A domain. Using a combination of a yeast two-hybrid screen coupled with deletion analysis, cAMP binding domain A of RI was dissected into two structurally and functionally distinct subsites, one that binds cAMP and another that binds the C subunit. The minimum stable subdomain required for binding to C in the 1-3 micromolar range is composed of residues 94-169, while residues 236-244, mapped to the C helix of cAMP binding domain A, were defined as a second surface necessary for high affinity (5-10 nanomolar) binding to C. This portion of the C helix, due to its position directly between the two subsites, serves as a molecular switch for either a cAMP-bound conformation or a C-bound conformation and can thus modulate interactions of cAMP binding domain A with cAMP, with C, and with cAMP binding domain B.     

Long-term progression in chronic manganism: ten years of follow-up

We studied the long-term clinical course of five patients with chronic manganese intoxication. The mean scores of the King's College Hospital Rating Scale for Parkinson's disease increased from 15.0 +/- 4.2 in 1987 to 28.3 +/- 6.70 in 1991 and then to 38.1 +/- 12.9 in 1995. The deterioration was most prominent in gait, rigidity, speed of foot tapping, and writing. Tissue concentrations of manganese in blood, urine, scalp hair, and pubic hair returned to normal. Follow-up MRIs did not show paramagnetic high-signal intensity on T1-weighted images. The data indicate that clinical progression in patients with manganese parkinsonism continues even 10 years after cessation of exposure.     

Self-stabilizing depth-first token circulation on networks

Summary This paper proposes a self-stabilizing protocol which circulates a token on a connected network in nondeterministic depth-first-search order, rooted at a special node. Starting with any initial state in which the network may have no token at all or more than one token, the protocol eventually makes the system stabilize in states having exactly one circulating token. With a slight modification to the protocol —by removing nondeterminism in the search — a depth-first-search tree on the network can be constructed. The proposed protocol runs on systems that allow parallel operations. Shing-Tsaan Huang was born in Taiwan on September 4, 1949. He got his Ph.D. degree in 1985 from Department of Computer Science, University of Maryland at College Park. Before he pursued his Ph.D. degree, he had worked several years in the computer industry in Taiwan. Professor Huang is currently the chairman of the Department of Computer Science, Tsing Hua University, Taiwan, Republic of China. His research interests include interconnection networks, operating systems and distributed computing. He is a senior member of the IEEE Computer Society and a member of the Association for Computing Machinery. Nian-Shing Chen was born in Taiwan on October 21, 1961. He received his Ph.D. degree in computer science from National Tsing Hua University in 1990. Dr. Chen is currently an associate professor with the Department of Information Management at Sun Yat-Sen University of Taiwan. His research interests include distributed systems, computer networks, computer viruses and expert systems. He is a member of IEEE and Phi Tau Phi honorary society.This research is supported by National Science Council of the Republic of China under the contract NSC81-0408-E-007-05 and NSC82-0408-E-007-027     

日本锰质合金生产与锰矿石的消耗

本文全面介绍了日本近年特别是1990年锰矿石进口量和锰铁合金的生产情况。同时还介绍了锰质合金、电解金属锰和电解二氧化锰的进出口情况。日本1990年进口锰矿石143万t,高碳锰铁4.6万t,中、低碳锰铁0.3万t,硅锰21.8t。还列出了进口矿石和硬质合金来源国数量。此外,还介绍了日本重点锰质合金厂的产品。     

液态金属钠高温沸腾实验回路应力分析与强度计算

本文主要完成了液态金属钠高温沸腾实验回路的应力分析与计算，确定了回路高温区连接管系的布置型式和支吊架系统，是该回路总体设计的重要组成部分。     

尺寸驱动的变参设计系统研究

本研究在简介国际上先进的变参设计几种方法及优点后,叙述了一个国家863/CIMS项目:CAD/CAPP/NCP集成系统中二维尺寸驱动的变参设计子系统的构成与功能;然后着重阐述了本系统采用的尺寸驱动的变参设计思想和几何约束建立的原则,并提供了系统数据结构,最后以几个实例证明了此方法的正确性与工程实用性。