Interactions between Cytochrome c and DNA Strands Self-Assembled at Gold Electrode

In this work, we reported the investigation on the interaction between DNA strands self-assembled at gold electrodes and an electron transfer protein, cytochrome c. We observed that cytochrome c exhibited well-defined electrochemistry in both double-stranded and single-stranded DNA films. This suggested that the electron transfer reaction of cytochrome c arose possibly due to the electron hopping along DNA strands rather than wiring along the double helix. We also compared the heterogeneous electron transfer rate of cytochrome c with that of a ruthenium complex, which further confirmed this mechanism.     

重组铜绿假单胞菌外毒素A工程菌发酵及表达产物的纯化

目的建立稳定、高效表达重组铜绿假单胞菌外毒素A(rEPA)的工程菌发酵及表达产物纯化工艺。方法规模化发酵表达rEPA的重组大肠杆菌rPE553D,离心收集菌体,渗透压调解使菌体周质间隙蛋白释放后,高速离心收集蛋白溶液。经DEAESepharoseFF、PhenylSepharose6FF疏水层析和SOURCE30Q强离子交换层析,超滤浓缩纯化rEPA。用HPLC、SDS-PAGE和Westernblot等方法检定生化和免疫学特性,并用小鼠和Vero细胞检定毒性。结果每55L培养基中rEPA产量超过4g,纯度在95%以上,细胞毒性降低了至少32000倍。其余各项指标均符合《中国生物制品规程》要求。结论已建立了收率高、纯度好、稳定、适合规模化生产rEPA的工艺。     

Phase Behavior and Mechanical Properties of Siloxane-Urethane Copolymer

Two series of siloxane-urethane copolymers were prepared from polydimethylsiloxane (PDMS) with a molecular weight of 1000 or 1800 which was used as a soft segment, 4,4′-diphenylmethane diisocyanate (MDI) and 1,4-butanediol (1,4-BD). Differential scanning calorimetry (DSC) demonstrated that the position (T_gs) and breadth (ΔB) of soft-segment glass transition of copolymers remained constant as the hard-segment content increased. Heat capacities at soft-segment glass transition of the copolymer (ΔCp) were 0.195∼0.411 J/g^○C and heat capacities of pure PDMS (ΔCp₀) were 0.571∼0.647 J/g^○C, leading to the various ΔCp/ΔCp₀ ratios. The ΔCp/ΔCp₀ ratios decreased as the increasing of hard-segment content, showing poor phase separation. The FTIR spectrum confirmed the occurrence of hydrogen bonding in ether end-group of pure PDMS. The ether group of the soft segment led to interfacial mixing between soft and hard segments. The tan δ of the soft segment determined by dynamic mechanical testing (DMA) also identified the mixing of soft and hard segments. The mechanical properties of the copolymer were directly related to either the soft and hard segment contents or the chain lengths of soft and hard segments. The hard segment that reinforced the soft segment and interfacial thickness between soft and hard segment dominated the mechanical properties.     

Preparation and characterization of high-surface-area titanium dioxide by sol-gel process

One of the important ways to improve photocatalytic efficiency is to prepare catalyst with enhanced surface area. In this work, titanium dioxide (TiO₂) nanoparticles having enhanced surface area were synthesized under the interference of SiO₂. The mixed oxide, SiO₂-TiO₂ (10% mol% Si), was prepared by a sol-gel procedure using titanium tetra-n-butoxide as Ti-precursor. The commercial SiO₂ nanoparticles were added into the TiO₂ sols after hydrolysis. After condensation and calcination heat treatment, the SiO₂-TiO₂ nanoparticles were obtained. To achieve the purpose of obtaining the high-surface-area TiO₂, the SiO₂ was removed subsequently by aqueous NaOH solution. The TiO₂ products were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), electron spectroscopy for chemical analysis (ESCA), and by N₂ adsorption-desorption isotherm. A fine mesoporous structure was formed for as-prepared TiO₂ after calcination at 400^∘C and the average pore diameter was about 7 nm. The porous TiO₂ products possess mixing phases of anatase and rutile. Phase transformation from anatase to rutile occurred when the samples were calcined. The phase transition temperature is sensitive to the silicon content. The particle size of ∼43 nm remained constant upon calcinations from 500 to 700^∘C. The specific surface area was increased up to 66% compared to regular TiO₂ samples that were prepared by the similar sol-gel procedure. The porous TiO₂ nanostructures exhibited enhanced photocatalytic performance to decompose methylene blue under UV irradiation.     

基于包围盒和空间分解的碰撞检测算法

文中提出一种基于包围盒和空间分解的碰撞检测算法,用以解决软体的碰撞检测。算法使用AABB包围盒做初步检测,确定可能发生碰撞的物体。再根据包围盒的重叠情况缩小可能发生碰撞的区域,利用哈希表作为数据储存结构进行空间分解,将物体包围盒重叠区域的基本几何元素的空间网格映射到哈希表中,将碰撞区域缩小到基本几何元素,最后用基元碰撞检测找出具体碰撞点。由于前期AABB包围盒的处理减少了空间分解阶段需要映射的基本几何元素数量,该算法具有较高的运算速度。     

面向语义网的中文本体应用研究

自从Protege出现以来,它成为唯一一款支持中文本体构建的工具,而且能够进行可视化操作。目前在部分领域已经构建了中文本体,但是对所构建本体在语义网中的应用缺乏研究。因为本体的应用主要依赖于推理工具,而国内尚无一款专业的基于中文本体的推理机或者推理工具,这阻碍了基于中文本体信息检索的发展。针对中文本体的应用,深入研究了本体在信息检索领域应用的现有技术、方法、工具。文中通过使用Jena开发包对中文本体的应用进行实验,实验表明,利用现有的推理工具等可以实现中文本体在语义网中的应用。     

Redox Properties and Catalytic Behavior of Praseodymium-Modified (Ce-Zr)O2 Solid Solutions in Three-Way Catalysts

The three-way catalyst promoters (Ce-Zr)O₂, (Pr-Ce-Zr)O₂ and (Pr-Zr)O₂ were prepared by the sol-gel method. The reduction/oxidation behavior of these mixed oxides was compared. It is shown that the formation of (Pr-Zr)O₂ cubic solid solution at high temperature up to 800 °C makes it more reducible, and that the ternary solid solution that formed in (Pr-Ce-Zr)O₂ mixed oxides plays an important role in the reduction process. The catalytic performance tests reveal that the introduction of a small amount of praseodymium into (Ce-Zr)O₂ favors the light-off temperature of C₃H₆ and NO and the effectiveness for NO conversion at the lean region.     

Influence of epoxy resin on the morphological and rheological properties of PBT/ABS blends compatibilized by ASMA

Polybutylene terephthalate (PBT)/acrylonitrile–butadiene–styrene (ABS) copolymer blends compatibilized by a mixture of styrene–acrylonitrile–maleic anhydride (ASMA) copolymers and epoxy resin (EP) were prepared through melt reactive extrusion. The morphological, rheological, and mechanical properties of these blends were studied. The epoxy functional groups of EP can react with anhydride groups of ASMA and the PBT terminal groups (? OH and ? COOH) simultaneously, leading to the formation of ASMA–EP–PBT graft copolymers. Because of the effective compatibilization of these copolymers at the interface, finer dispersed phase morphologies were obtained. Compared with PBT/ABS/ASMA blends, the addition of EP induced a more stable molten phase structure, with increases of storage moduli, loss moduli, and dynamic viscosities. Results indicated that 1.5 wt% of the EP in the blends was most suited for the compatibilization. Impact properties of these blends were also investigated. POLYM. ENG. SCI., 47:1943–1950, 2007. © 2007 Society of Plastics Engineers     

福建三钢焦化废水处理工艺的改造

通过对福建三钢煤化工有限公司废水处理系统生物脱氮工艺的改造，使出水水质达到国家规定的一级排放标准。同时，对焦化废水处理工艺流程、设备及工艺参数等方面进行了调整和改进，为焦化废水生物脱氮工艺的设计和运行提供了借鉴。     

Fabrication of high surface area graphitic nanoflakes on carbon nanotubes templates

Graphitic nanoflakes were fabricated on the carbon nanotubes templates for increasing the surface area utilizing bias assisted microwave plasma enhanced chemical vapor deposition (MWPECVD). The analysis of morphologies and structures were achieved by means of scanning electron microscopy and transmission electron microscopy. The surface area of graphitic nanoflakes, carbon nanotubes (CNTs) and graphitic nanoflakes/CNTs were 57.44 m²/g, 90.31 m²/g and 130.96 m²/g from BET measurement, respectively. The cyclic voltammetry was used to calculate the active area of platinum catalysts in 1 M sulfuric acid from hydrogen adsorption peak. An enhancement of activity could be observed from the calculation of CV results. This may be attributed to the small particle size and high dispersion of platinum particles coated on graphitic nanoflakes/CNTs. These high surface area materials could be used as catalysts supports or electrode for fuel cell applications.