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31.
32.
Activated endothelial cells and stimulated platelets express the cell adhesion molecule P-selectin (CD62P), which mediates adhesion to various leukocytes and certain types of cancer cells. In this study, we show Ca2+-dependent binding of P-selectin to NKI-4 cells, a cell line derived from a human melanoma. The binding is inhibited by P7 (a leukocyte adhesion blocking mAb against P-selectin), but not by PL5 (a leukocyte adhesion blocking mAb against P-selectin glycoprotein ligand-1; PSGL-1). Further, expression of PSGL-1 could not be detected on NKI-4 cells by either PL5 mAb or an Ab against a synthetic peptide corresponding to a portion of the PSGL-1 sequence. P-selectin affinity chromatography of lysates from in vivo [3H]-glucosamine-labeled NKI-4 cells resulted in the isolation of three glycoproteins, with apparent molecular masses of approximately 250, approximately 110, and approximately 100 kDa under reducing conditions and approximately 230, approximately 105, and approximately 85 kDa under nonreducing conditions. These molecules could be precipitated by P-selectin, but not by E-selectin. EDTA and the P7 mAb, but not the PL5 mAb, inhibited the binding of P-selectin to the purified ligands. Surprisingly, we found that sodium chlorate, a sulfation inhibitor, did not inhibit the binding of P-selectin to NKI-4 cells and that [35S]-sulfate did not label the NKI-4 cell ligands. We conclude that P-selectin-dependent adhesion of the human melanoma cell line NKI-4 is mediated by a novel class of glycoprotein ligands. 相似文献
33.
Gao Fang Jia Xiaofeng Zhao Zhiyun Chen Chih-Cheng Xu Feng Geng Zhe Song Xiaotong 《Microsystem Technologies》2021,27(4):1545-1557
Microsystem Technologies - Artificial intelligence (AI), together with its applications, has received world-wide attentions and is expected to exert force on the development of global economy and... 相似文献
34.
We introduce a novel method for synthesizing dance motions that follow the emotions and contents of a piece of music. Our method employs a learning-based approach to model the music to motion mapping relationship embodied in example dance motions along with those motions' accompanying background music. A key step in our method is to train a music to motion matching quality rating function through learning the music to motion mapping relationship exhibited in synchronized music and dance motion data, which were captured from professional human dance performance. To generate an optimal sequence of dance motion segments to match with a piece of music, we introduce a constraint-based dynamic programming procedure. This procedure considers both music to motion matching quality and visual smoothness of a resultant dance motion sequence. We also introduce a two-way evaluation strategy, coupled with a GPU-based implementation, through which we can execute the dynamic programming process in parallel, resulting in significant speedup. To evaluate the effectiveness of our method, we quantitatively compare the dance motions synthesized by our method with motion synthesis results by several peer methods using the motions captured from professional human dancers' performance as the gold standard. We also conducted several medium-scale user studies to explore how perceptually our dance motion synthesis method can outperform existing methods in synthesizing dance motions to match with a piece of music. These user studies produced very positive results on our music-driven dance motion synthesis experiments for several Asian dance genres, confirming the advantages of our method. 相似文献
35.
配电网三相负荷不平衡给电力系统和用户带来经济损失和风险.当前,换相开关型三相负荷自动调节装置广泛使用于配电网,以解决负荷三相不平衡问题.为验证现有换相开关型三相负荷自动调节装置的逻辑正确性和其他换相性能是否符合实际应用要求,设计并研制了一款换相开关型三相负荷自动平衡装置检测系统,通过判断换相过程中采样得到的有关电气量是否满足待测装置技术规范要求的检测方法,以实现快速方便的检测工作.为验证所设计检测装置的有效性和实用性,用其对市面上的智能换相开关进行测试,最终得出所提检测装置能够快速方便地判断待测装置换相逻辑正确与否以及其他换相性能是否满足要求的结论. 相似文献
36.
A multifunctional thiophene-based covalent triazine framework (TTPATTh) with triphenylamine as core is synthesized by Friedel–Crafts reaction of cyanuric chloride with thienyl derivative for the first time. The yield of TTPATTh (99.59%) is far higher than that of the carbazole-based CTF (TTPATCz, 47.03%). TTPATTh and TTPATCz possess high BET surface areas with 1235 and 2501 m2 g–1 as well as high pore volumes with 1.60 and 2.23 cm3 g–1, respectively. TTPATTh and TTPATCz have high thermal stability with high thermal decomposition temperatures of 514 and 598 °C in nitrogen atmosphere. With the introduction of triazine rings, the fluorescence sensing sensitivities of TTPATTh and TTPATCz to picric acid and iodine are improved significantly. Especially for TTPATTh, the Ksv values reach 5.95 × 105 and 1.61 × 104 L mol–1, and LODs reach 1.02 × 10–12 and 1.86 × 10–12 mol L–1. To the best of the knowledge, this is the most sensitive value among thiophene-based porous organic polymers to PA and I2. Furthermore, TTPATTh and TTPATCz can also adsorb iodine in vapor phase, cyclohexane solution and aqueous solution, and release iodine by heating or in ethanol solution. The efficiency of controlling the release of iodine is higher than that in ethanol. 相似文献
37.
38.
贵金属的化学发光分析 总被引:8,自引:1,他引:8
介绍化学发光分析的基本原理和测试技术,及其应用于测定贵金属的几种化学发光反应,并展望其前景。 相似文献
39.
研究了Ce对Al—16%Si合金熔体粘滞性的影响。结果表明:Ce的加入增加了合金熔体的粘度,且随着Ce含量的增加粘度值也增加。结合加入Ce的Al—16%Si合金氢含量的变化、凝固组织和DSC曲线研究了熔体结构的变化,分析了粘度与液态结构变化之间的关系。 相似文献
40.
通过对飞机发动机机罩概念设计中的物-场分析,简要概括了在实际的概念设计中应该如何应用和把握物质-场分析的侧重点。 相似文献