Fibrous myopathy. A rheumatic complication of drug abuse

A progressive fibrous myopathy may result from chronic intramuscular drug abuse. This complication may mimic other rheumatic disorders and early recognition may prevent disability. The patient described here presented with fixed flexion and extension contracture of hips and knees, respectively, after abusing meperidine and other agents for 3 years. Soft tissues of thighs and buttocks were "wood hard," EMG showed absence of action potentials in affected muscles, and biopsy revealed extensive replacement of muscle with dense, acellular fibrous tissue. Possible mechanisms are discussed.     

Effect of the CO-2-bicarbonate buffer system on the water and ion contents of rat renal cortical slices

(1) Partial replacement of medium chloride by bicarbonate, with pHmaintained constant by addition of CO2, lead to a greater rate of swelling when rat renal corti cal slices were leached in nitrogenated medium at 0--2 degree C. This greater rate of swelling was still present when 1 mM iodoacetate was added to the leaching media. (2) Rat renal cortical slices incubated in oxygenated bicarbonate medium at 25 degree C maintained a slightly greater tissue water content than did slices incubated in phosphate-buffered media. Inclusion of malate in the incubation medium also led to a greater steady-state water content when slices were incubated at 25 degree C in oxygenated media. These results suggest that that particular cell volume which is maintained by metabolism and the double Donnan system is determined at least in part by the nature of the extracellular anions. (3) The rates of net sodium extrusion and of net potassium uptake were not significantly altered by presence of CI2 and bicarbonate in the incubation medium.     

The post-suicide family and the family physician

It is estimated that there are 750,000 people each year who are intimately affected by suicide. Prominent among these are the family survivors and their family physician. This paper offers a time frame which divides the period following the suicide into three phases: Immediate (the first ten days after the suicide); Intermediate (after the first ten days through the first year); and Extended (from the first year until restitution occurs). It identifies the chief emotional reactions which occur in each phase, explores their psychodynamic origins, and proposes suggestions for appropriate management during each of the three periods. The goal of this plan of management is to enable the family physician to function in a supportive emphatic, and restorative manner for the post-suicide family.     

The permeability of ternary particulate mixtures for laminar flow

Experimental measurements are reported of permeabilities of beds of ternary mixtures of 2, 1 and 0.5 mm spherical glass beads under laminar flow conditions. The results are presented in the form of ternary diagrams of isopermeabilities from which the standard permeability of any mixture in the ternary system used can be read off directly. As no ternary laminar flow permeability data exist in the literature, no comparison with other work could be made, but reference is made to the published work on ternary turbulent flow permeability. It is shown that the permeabilities in both cases are influenced by the same bed properties and that the particle size and shape have a much greater relative effect on laminar flow permeability than on its turbulent counterpart. It is also concluded that the standard permeability under laminar flow may be obtained from eqn. (3) by inserting appropriate values of the friction factor, the mean particle size and shape, and the bed porosity.     

Picosecond time-interval measurement and intensity correlations using the two-quantum photoelectric effect

A technique based on the two-quantum photoeffect has been used to measure second-order intensity correlations, pulse shapes from a mode-locked argon ion laser, and time delays in the picosecond domain. Good agreement was obtained between the mode distribution computed from the intensity autocorrelation data and that observed with a scanning Fabry-Perot when the laser was run in the mode-locked condition. Additionally, the contrast ratios observed in both the mode-locked and free-running condition were in good agreement with a theory by Weber and Danielmeyer.     

Inhibition of colon adenocarcinoma cell proliferation by flavonols is linked to a G2/M cell cycle block and reduction in cyclin D1 expression

The moderate consumption of red wine has been proposed to reduce the incidence of colorectal cancer. Its anti-cancer action my in part be mediated by the actions of polyphenols on colon cancer epithelial cells. We show that a red wine phenolic (50 μg/ml) extract devoid of anthocyanins (removed to reflect polyphenol bioavailability to the large intestine) and the major red wine flavonols quercetin, myricetin, laricitrin and syringetin (50 μM) are capable of inhibiting the proliferation of colorectal epithelial adenocarcinoma cells. This anti-proliferative activity was partly mediated by the direct cytotoxic actions of flavonols and also their ability to cause a significant cell cycle blockage in G2/M. The anti-proliferative effects induced by flavonols were preceded, in most cases, by a strong and significant reduction of cyclooxygenase-2 (COX-2) and cyclin D1 expression. These results indicate that the observed inverse correlation between colon cancer and a moderate red wine intake may be partly mediated by the actions of red wine flavonols on the growth of cancer cells.     

Using shear force,sarcomere length,particle size,collagen content,and protein solubility metrics to predict consumer acceptance of aged beef tenderness

In this study, the relationship between sensory evaluation and several objective metrics of beef tenderness was tested. Objective metrics included shear force, sarcomere length, collagen content, myofibrillar, and sarcoplasmic protein solubility and particle size analysis. These results were compared to consumer panel scores of tenderness for the same aged beef striploin (longissimus lumborum muscle) samples. There was found to be a significant relationship between sarcomere length, shear force, and particle size with tenderness scores. Collagen content and protein solubilities were not associated to tenderness scores (p > 0.05). Sarcomere length contributions for explaining tenderness variation were overlapped by the contributions of shear force (collinearity). Independent models demonstrated that the lower 95% confidence interval of the fitted regression line exceeded 50% acceptance of tenderness when shear force values <42.6 N and when particle size values <198 μm. We can recommend these as thresholds for consumer acceptance of beef tenderness, although considerations of sample type, analytical methodology, and consumer demographics should be made prior to their adoption. This provision was based on the variation in tenderness scores evident between individual panelists and experimental striploins.     

α-Amylases in raw and fermented African oil bean seeds (Pentaclethra macrophylla benth)

The !-amylases of raw and fermented seeds of African oil bean (Pentaclethra macrophylla Benth) were isolated, partially purified (tenfold) and subjected to different thermal and pH conditions. The specific activities of the purified enzyme from raw and fermented seeds were 0.037 ml^-1 min^-1 and 0.88 ml^-1 min^-1 respectively. The !-amylase from fermented seeds was more thermostable with optimum activity at 70 °C, compared to the optimum temperature of 60 °C obtained for the raw seed enzyme. The assayed !-amylases were stable over a wide range of pH (3.0-7.0), with optimum activity found at pH 6.0 and pH 5.0 for raw and fermented seeds respectively. The results show that the !-amylase of Pentaclethra macrophylla complements the microbial amylases in the mainly bacterial fermentation of African oil bean seeds to 'ugba', a highly nutritious food condiment. While the !-amylase in the raw seeds was basically the plant enzyme, that isolated from the fermented seeds was a combination of plant and microbial !-amylases.