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11.
进入更广泛的历史语境探讨清乾隆时期镜清斋假山的营造思路,追溯至晚明江南的两种假山营造来源.在整体构思上,承续了17世纪前期张南垣所创的追求画意真山的"天然"假山;而在局部营造中,则采纳了16世纪后期盛行的追求胜景无穷的"人巧"假山.除镜清斋之外,在稍晚的苏州环秀山庄假山中也有显著表达,是清代假山发展高峰的体现.清末和1970年代对该假山的两次改造,未能很好理解原初的思路取向,一定程度上损害了当初追求的山意. 相似文献
12.
Stefanie Vanbecelaere Katrien Van den Berghe Frederik Cornillie Delphine Sasanguie Bert Reynvoet Fien Depaepe 《Journal of Computer Assisted Learning》2020,36(4):502-513
For the training of academic skills, digital educational games with integrated adaptivity are promising. Adaptive games are considered superior to non-adaptive games, because they constantly assess children's performance, and accordingly adapt the difficulty of the tasks corresponding to the children's individual level. However, empirical evidence with regard to the effectivity of adaptive compared to non-adaptive games is limited. A study was conducted with 191 children from the third year of Kinder garten who were enrolled in one of three conditions, that is, playing an adaptive version of the reading game (RG), a non-adaptive version of the RG or training with pen-and-paper exercises. In all three conditions, children trained emergent reading (phonological awareness and letter knowledge) once a week for 30 min over a period of 5 weeks. Children's performance on cognitive (phonological awareness, letter knowledge, reading fluency) and non-cognitive (motivation, self-concept) factors was assessed. Results revealed a significant improvement in phonological awareness and letter knowledge in all conditions. However, no differences between the conditions were observed with respect to children's improvement on phonological awareness and letter knowledge or on their post-test scores for reading fluency. With regard to motivation and self-concept, again, no differences in these non-cognitive factors were observed across conditions. 相似文献
13.
Kai He Zhe Li Longfei Liu Mengxi Zheng Prof. Chengde Mao 《Chembiochem : a European journal of chemical biology》2020,21(15):2132-2136
As a giant leap in DNA self-assembly, DNA origami has exhibited an unprecedented ability to construct nanostructures with arbitrary shapes and sizes. In typical DNA origami, hundreds of short DNA staple strands fold a long, single-stranded (ss) DNA scaffold cooperatively into designed nanostructures. However, large numbers of DNA strands are expensive and would hinder applications such as pharmaceutical investigations because of the complicated components. Therefore, one challenge is how to reduce the number of staple strands needed to construct DNA origami. For a DNA origami structure, the scale-free folding pattern of the scaffold strand is determined by staple strands at the branching vertexes. Simple duplex regions help to define the size-related features of the origami geometry. In this study, we hypothesized that a scaffold strand can be correctly folded into a designed topology by using only staple strands involved in branching vertexes. After assembly, any remaining, flexible, single-stranded regions of the scaffold could be converted into rigid duplexes by DNA polymerase to achieve the designed geometric structures. To demonstrate the concept, we used only 18 staple strands (covering 15 % of the scaffold strand) to assemble a porous DNA nanostructure, which was visualized by atomic force microscopy (AFM). This study helps understanding of the role of cooperativity in origami folding, and provides a cost-effective approach for small-scale prototyping DNA origami. 相似文献
14.
Sebastian Roth Peter Stockinger Jakob Steff Simon Steimle Dr. Viktor Sautner Prof. Dr. Kai Tittmann Prof. Dr. Jürgen Pleiss Prof. Dr. Michael Müller 《Chembiochem : a European journal of chemical biology》2020,21(18):2615-2619
The family of NAD(P)H-dependent short-chain dehydrogenases/reductases (SDRs) comprises numerous biocatalysts capable of C=O or C=C reduction. The highly homologous noroxomaritidine reductase (NR) from Narcissus sp. aff. pseudonarcissus and Zt_SDR from Zephyranthes treatiae, however, are SDRs with an extended imine substrate scope. Comparison with a similar SDR from Asparagus officinalis (Ao_SDR) exhibiting keto-reducing activity, yet negligible imine-reducing capability, and mining the Short-Chain Dehydrogenase/Reductase Engineering Database indicated that NR and Zt_SDR possess a unique active-site composition among SDRs. Adapting the active site of Ao_SDR accordingly improved its imine-reducing capability. By applying the same strategy, an unrelated SDR from Methylobacterium sp. 77 (M77_SDR) with distinct keto-reducing activity was engineered into a promiscuous enzyme with imine-reducing activity, thereby confirming that the ability to reduce imines can be rationally introduced into members of the “classical” SDR enzyme family. Thus, members of the SDR family could be a promising starting point for protein approaches to generate new imine-reducing enzymes. 相似文献
15.
从清水混凝土材料特点、空间适用性、肌理、细节和工艺等方面总结了荣成青少年活动中心复杂异形清水混凝土工程从设计到建造的全过程,展现了本土设计理念和地域环境的融合、创新型施工工艺的成功应用及项目最终呈现的高品质完成度. 相似文献
16.
Kang Zhang Rong‐rong Wang Kai‐rui Zhang Fei Cheng Yu Tian Yi Lin Mi Zhou Pu‐xin Zhu 《Polymer International》2020,69(3):274-279
A series of hyperbranched poly(citric polyethylene glycol) (PCPEG) materials with varied polyethylene glycol (PEG) chain lengths as plasticizers were mixed with maize starch (MS) via cooking and film‐forming. The structure, pasting property, plasticization, aging property, moisture absorption and compatibility of plasticized starches were studied by means of Fourier transform infrared spectroscopy, X‐ray diffraction, rapid viscosity analysis, tension testing, moisture absorption measurements and scanning electron microscopy. Compared with PEG and citric acid, PCPEG was more effective in promoting starch chain movement and inhibiting the retrogradation of starch film. Also, PCPEG/MS had smaller moisture content. The longer the plasticizer chain, the better were the aging resistance and moisture resistance of starch. But with an increase of PEG chain length, mechanical properties of PCPEG/MS deteriorated and the compatibility between PCPEG and MS decreased. The hyperbranched derivative of PEG with longer chain exhibited improved plasticization and compatibility with starch. © 2019 Society of Chemical Industry 相似文献
17.
介绍了尿素装置中3起液位仪表失真案例,分析了故障现象和设备工况,确定失真原因,采取了相应的处理措施,解决了仪表故障。 相似文献
18.
江河湖海疏浚工程对于拓展和保障航运安全、促进生态恢复、维护/改善远海岛礁环境具有不可或缺的作用.目前,在绞吸和耙吸疏浚船上,多采用射线密度计及电磁流量计对输泥密度、产量等进行在线计量,并作为实现疏浚系统自动控制的重要参量.由于射线密度计潜在的人员健康、公共安全和环保风险,在一些国家和地区已建立了放射源的有序退出机制.电阻层析成像使用安装于管壁的电极阵列获得管道内流体的电导率分布,进而推导出管道内的泥浆密度,具有无辐射、全场测量及实时可视化的优点.电阻层析成像测量系统经过特殊设计,可适应从内陆淡水到海水的巨大电导率变化.初步测试表明,电阻层析成像测量数据与射线密度计具有良好一致性,具备了作为在线泥浆密度测量的可行性.此外,ERT可提供实时的流动成像,对于提高对疏浚管道监测能力和降低堵管概率具有重要意义. 相似文献
19.
Anh Tuan Thanh Pham Oanh Kieu Truong Le Dung Van Hoang Truong Huu Nguyen Trang Huyen Cao Pham Phuong Thanh Ngoc Vo Thang Bach Phan Vinh Cao Tran 《Journal of the European Ceramic Society》2021,41(6):3493-3500
Structure modification has been found to tune significantly the transparent-conducting performance, especially mobility and conductivity of hydrogenated Ga-doped ZnO (HGZO) films. The strong correlation between film thickness and mobility of the films is revealed. The mobility increases quickly with increasing the thickness from 350 to 900 nm, and then tends to be saturated at further thicknesses. A higher mobility than 50 cm2/Vs can be achieved, which is an extra-high value for polycrystalline ZnO films deposited by using the sputtering technique. The thickness-dependent mobility originates from scatterings on grain boundaries and dislocation-induced defects controlled by thin-film growth. Based on the Volmer-Weber model, an expansion model is built up to describe the thickness-dependent crystal growth of the HGZO films, especially at the thick films. As a result, the 800 nm-thick HGZO film obtains the highest performance with high mobility of 51.5 cm2/Vs, low resistivity of 5.3 × 10?4 Ωcm, and good transmittance of 83.3 %. 相似文献
20.
在现有的时间银行系统中,时间币的发行功能和结算功能完全集中到一个中心节点上。这种极度中心化的功能结构,不仅存在容易发生中心节点单点失效、数据容易被篡改等信息安全问题,还存在着时间币的发行和流通缺乏透明度以及时间币的结算依赖中心化的结算机构等问题。针对这些问题,提出了一种基于区块链技术的解决方法。首先,将时间币的发行功能和结算功能从中心节点上分离出来;然后,利用具有分布式去中心化、集体维护和不可篡改等特性的区块链技术,将分离出来的发行功能逐步去中心化,将分离出来的结算功能去中心化,形成公益时间链(PWTB);最终,PWTB利用区块链技术以去中心化的方式将时间银行系统由单个节点维护账本变成由集体维护一个分布式的共享账本,使时间币的发行和流通公开透明,时间币的结算不依赖某个中心化的节点。安全分析表明所设计的PWTB能够实现安全的信息传输与存储,以及数据的共享。 相似文献