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排序方式: 共有553条查询结果,搜索用时 13 毫秒
21.
Shoji Seki Mami Iwasaki Hiroto Makino Yasuhito Yahara Yoshitaka Miyazaki Katsuhiko Kamei Hayato Futakawa Makiko Nogami Nguyen Tran Canh Tung Tatsuro Hirokawa Mamiko Tsuji Yoshiharu Kawaguchi 《International journal of molecular sciences》2022,23(7)
Intervertebral disc (IVD) diseases are common spinal disorders that cause neck or back pain in the presence or absence of an underlying neurological disorder. IVD diseases develop on the basis of degeneration, and there are no established treatments for degeneration. IVD diseases may therefore represent a candidate for the application of regenerative medicine, potentially employing normal human dermal fibroblasts (NHDFs) induced to differentiate into nucleus pulposus (NP) cells. Here, we used a three-dimensional culture system to demonstrate that ectopic expression of MYC, KLF4, NOTO, SOX5, SOX6, and SOX9 in NHDFs generated NP-like cells, detected using Safranin-O staining. Quantitative PCR, microarray analysis, and fluorescence-activated cell sorting revealed that the induced NP cells exhibited a fully differentiated phenotype. These findings may significantly contribute to the development of effective strategies for treating IVD diseases. 相似文献
22.
The objective of this study is to evaluate simultaneously the time dependence of the thermal diffusivity of carbon-carbon composites (C/C composites) and their porosity during heat treatment using the electromagnetic ultrasonic technique. This paper describes two kinds of experiments conducted to confirm the principle for simultaneous measurement of both the ultrasonic velocity (used to evaluate the porosity) and the thermal diffusivity at room temperature. For each material, the samples used in both experiments were identical. The ultrasonic velocity of type 304 stainless steel and its thermal diffusivity were 5.85 km/s and 3.8 mm2/s with precisions of ±1.6 and ±8 percent, respectively. The ultrasonic velocity of a two-dimensional woven C/C composite and its thermal diffusivity were 2.86 km/s and 4.8 mm2/s with precisions of ±5.0 and ±8 percent, respectively. The results appear to indicate that the electromagnetic ultrasonic technique can measure the ultrasonic velocity and the thermal diffusivity simultaneously and that it is also applicable to C/C composites. © 1997 Scripta Technica, Inc. Heat Trans Jpn Res, 25(5): 308–322, 1996 相似文献
23.
Daniel T. Payne Mandeep K. Chahal V clav B&rcaron ezina Whitney A. Webre Katsuhiko Ariga Francis D Souza Jan Labuta Jonathan P. Hill 《Frontiers of Chemical Science and Engineering》2020,14(1):28
Chiral 1,1’-binaphthyl-linked diporphyrin ‘tweezers’ (R)-1/(S)-1 and the corresponding zinc(II) complexes (R)-2/(S)-2 were prepared as chiral host molecules, and their utility for chiral analyses (especially enantiomeric excess (ee) determinations) were evaluated. Tris(1-n-dodecyl)porphyrins were used for the first time as the interacting units. Host capabilities of the diporphyrin tweezers were investigated by titrations with (R,R)- and (S,S)-cyclohexane-1,2-diamine (CHDA). The host molecules could be used as multichannel probes of ee by using UV-vis, circular dichroism (CD), fluorescence emission and 1H nuclear magnetic resonance (1H-NMR) methods. Chiral configurations could also be differentiated using CD or 1H-NMR spectroscopy. All three optical techniques give good resolution of ee with reasonable sensitivity considering the low concentrations used (ca. 10−6 mol·L−1). The ee determination of CHDA enantiomers using NMR spectroscopy is also possible because of the reasonably well separated resonances in the case of (R,R)- and (S,S)-CHDA. Non-metallated (R)-1/(S)-1 hosts could not be used to detect chiral information in a strongly acidic chiral guest. This work demonstrates the utility of 1,1’-binapthyl-linked chiral hosts for chiral analysis of ditopically interacting enantiomers. 相似文献
24.
Pitting potentials and stress corrosion life-times of AlCuMg alloys (mainly 2024 alloy) with various ageing structures have been measured in a de-aerated 1M NaCl solution under conditions of controlled potential. The aged alloy, which has the higher susceptibility to stress-corrosion cracking, showed two pitting potentials corresponding to pitting at the grain boundaries and within the grains. The susceptibility of the alloys to intergranular stress-corrosion cracking occurred at potentials above the pitting potential of the grain boundaries. The intergranular stress-corrosion cracking is caused not by the dissolution of the grain boundary precipitates (S phase) but by the pitting dissolution of the solute-denuded zones along the grain boundaries. Aspects of SCC in the alloys are similar to those in the Al-4%Cu alloy without Mg. 相似文献
25.
Takuji Kobayashi Katsuhiko Sasaki 《Journal of Materials Science: Materials in Electronics》2009,20(4):343-353
This paper discusses uniaxial ratchetting deformation of lead-free solder alloy Sn–3Ag–0.5Cu and lead-containing solder alloy
Sn–37Pb, which were subjected to tension–compression loading with several stress amplitudes and stress ratios, minimum stress
over maximum stress. First the uniaxial ratchetting tests were conducted with three maximum stresses and four stress ratios.
All tests were conducted using cylindrical bulk specimens of the solder alloys at 313 K. The test results show that there
are differences in the ratchetting deformation behavior of the two solder alloys; the larger ratchetting strain occurs in
the lead-containing solder alloy than in the lead-free solder alloy. The ratchetting deformation was simulated by the dislocation
based constitutive model proposed by Estrin et al. (J Eng Mater Technol 118:441, 1996). The evolution equation of the back
stress employed in the constitutive model was modified considering a dynamic recovery term. The effect of the modification
of the back stress evolution is discussed by comparing the simulations with the corresponding experimental results. The simulations
suggest that the recovery term in the kinematic hardening rule plays an important role in fitting the simulation to the experimental
results of the ratchetting deformation of the solder alloys. 相似文献
26.
Teruhisa Horita Haruo Kishimoto Katsuhiko Yamaji Manuel E. Brito Yueping Xiong Harumi Yokokawa 《Journal of power sources》2009,193(1):180-184
Reactivity of oxide scale on Fe-Cr alloy with Na-containing gases was examined to estimate the stability against sodium (Na): vapors of NaCl and Na2SO4 exposures with air flow at 1073 K. The identified reaction phases were Cr-Mn spinel, Cr2O3, and alloy from the X-ray diffraction of surface with no Na-reaction products. However, the protective oxide scales (Mn-Cr spinel and Cr2O3 layers) on the Fe-Cr alloy were partially decomposed by reacting with Na to form Na-compounds inside the oxide scale/alloy interfaces. In some parts, anomalous oxide scales were found around the oxide scale/Fe-Cr alloy interfaces, with forming Na-rich compounds: the compounds were distributed inner parts of oxide scales around oxide scale/alloy interfaces. The stability of oxide scales and degradation were discussed based on the observed distribution of elements. 相似文献
27.
28.
Katsuhiko Sano 《Journal of Logic, Language and Information》2009,18(4):515-539
The purpose of this paper is to argue that the hybrid formalism fits naturally in the context of David Lewis’s counterfactual
logic and that its introduction into this framework is desirable. This hybridization enables us to regard the inference “The
pig is Mary; Mary is pregnant; therefore the pig is pregnant” as a process of updating local information (which depends on
the given situation) by using global information (independent of the situation). Our hybridization also has the following
technical advantages: (i) it preserves the completeness and decidability of Lewis’s logic; (ii) it allows us to characterize
the Limit Assumption as a proof-rule with some side-conditions; and (iii) it enables us to establish a general Kripke completeness
result by using the proof-rule corresponding to the Limit Assumption. 相似文献
29.
Avidin-containing polyelectrolyte microcapsules were prepared by a layer-by-layer deposition technique and uptake and release
of biotin-labelled fluorescein (b-FITC) was studied. The polyelectrolyte microcapsules were prepared by coating the surface
of calcium carbonate (CaCO3) microparticles containing avidin-poly(styrene sulfonate) (PSS) conjugate, followed by dissolution of CaCO3 core in an ethylenediaminetetraacetic acid solution. Release of avidin from the microcapsules was markedly suppressed due
to formation of a high molecular weight of avidin-PSS conjugate in the microcapsules. The uptake of b-FITC into the microcapsules
was highly enhanced through a strong binding of b-FITC to avidin, as compared to the uptake into avidin-free microcapsules.
Release of b-FITC from the microcapsules was accelerated upon addition of biotin, 2-iminobiotin, or lipoic acid in the solution
due to the competitive binding of the additives to the binding site of avidin. 相似文献
30.
Suzuki K Sami M Kadokura H Nakajima H Kitamoto K 《International journal of food microbiology》2002,76(3):223-230
We isolated a strain from hop-resistant Lactobacillus hrevis ABBC45, which had lost a plasmid (pRH45) harboring a putative hop resistance gene, horA. The hop resistance level of this horA-deficient strain, named ABBC45(C), was initially low but gradually induced by repeated growth in media containing progressively increasing levels of hop compounds. Although the hop resistance level was substantially lower than that of the hop-adapted wild type strain, hop-adapted ABBC45(C) (ABBC45(CR)) was still capable of growing in beer, suggesting ABBC45 possesses at least two hop resistance mechanisms. Hop resistance acquired by ABBC45(CR) gradually diminished to the pre-adapted level, when the strain was grown repeatedly in the absence of hop compounds. ABBC45(CR) was found to be cross-resistant to several structurally unrelated drugs, including ethidium bromide, daunomycin and nisin. In addition, ABBC45(CR) was shown to extrude ethidium in an energy-dependent manner, while ABBC45(C) did not show such activity. This indicates that the efflux pump was induced by adaptation to hop compounds. The efflux activity of ethidium was reduced by the addition of hop compounds, suggesting hop compounds are also the substrate of the efflux pump. It was also shown that the efflux activity was completely dissipated with the abolition of proton motive force (PMF). These results, taken together, suggest the hop resistance mechanism of ABBC45(C) is mediated by PMF-dependent multidrug efflux pump. 相似文献