Effect of Oxygen on the Self-formation of Carbonaceous Tribo-layer with Carbon Nitride Coatings under a Nitrogen Atmosphere

The ball-on-disk friction and wear tests of CN _X coatings (CN _X /CN _X ) were conducted under a nitrogen atmosphere with controlled relative humidity (RH) (3.4–40.0%RH) and oxygen concentration (100–21 × 10⁴ ppm) in this study. We found that the specific wear rate of CN _X coating on ball (W _b), which could give stable and low friction coefficient (<0.05), was below 3.0 × 10^?8 mm³/Nm. Average friction coefficients (µ _a) and W _b of CN _X /CN _X increased (µ _a: 0.02–0.33, W _b: 1.6 × 10^?8–2.4 × 10^?7 mm³/Nm) with increasing oxygen concentration (230–211,000 ppm) as well as RH (4.7–21.1%RH) under a nitrogen atmosphere. However, the W _b remained low value below 2.3 × 10^?8 mm³/Nm regardless of oxygen concentration (100–207,000 ppm) of a nitrogen atmosphere (3.4–3.9%RH) when CN _X -coated balls were slid against a hydrogenated CN _X (CN _X :H) coatings (CN _X /CN _X :H). Besides, the CN _X /CN _X :H achieved low and stable friction coefficient below 0.05 under a nitrogen atmosphere (10,000 ppmO₂) regardless of increasing RH up to 20%RH. Raman analysis indicated that the structure of carbon on the top surface of CN _X coating was changed from as-deposited CN _X coating in the case of low friction coefficient (<0.05). Furthermore, TOF-SIMS analysis provided the evidence that the carbon derived from CN _X -coated disk was considered to diffuse into the ball surface, and it mixed with the carbon derived from CN _X -coated ball on the wear scar, which formed the chemically bonded carbon tribo-layer. Low friction coefficient (<0.05) with CN _X coatings under a nitrogen atmosphere was achieved due to self-formation of the carbon tribo-layer.     

Enhanced growth inhibition of hepatic multicellular tumor spheroids by lactosylated poly(ethylene glycol)-siRNA conjugate formulated in PEGylated polyplexes

PEGylated polyplexes (lac-PEGylated polyplexes) composed of poly(L-lysine) and lactosylated poly(ethylene glycol)-small interfering RNA conjugate, which inhibits the RecQL1 gene product, were revealed to show an appreciable growth inhibition of multicellular HuH-7 spheroids (human hepatocarcinoma cell lines) for up to 21 days (IC(50)=6 nM); this system used as an in vitro three-dimensional (3D) model mimicking the in vivo biology of tumors. The PEGylated polyplexes thus prepared had a size of approximately 110 nm with clustered lactose moieties on their periphery as targeting ligands for the asialoglycoprotein-receptor-expressing HuH-7 cells. In contrast, OligofectAMINE/siRNA (cationic lipoplex) was observed to have almost no growth-inhibitory effect against HuH-7 spheroids, even though the lipoplex showed a stronger growth-inhibitory effect than the lac-PEGylated polyplexes on conventional monolayer-cultured HuH-7 cells. The FITC-tagged conjugate in the lac-PEGylated polyplexes showed smooth penetration into the HuH-7 spheroids compared with that in the lipoplexes, as observed by confocal fluorescence-scanning microscopy. This indicates that the small size of approximately 100 nm and the reduced nonspecific interaction due to the nonionic and hydrophilic lactosylated PEG layer contributes to the smooth penetration of the PEGylated polyplexes into the spheroid interior, eventually facilitating their uptake into the cells composing the spheroids. Cellular apoptosis indicating programmed cell death was also observed in the HuH-7 spheroids treated with the PEGylated polyplexes, revealing that the observed growth inhibition was indeed induced by the RNAi of the RecQL1 siRNA. These data suggest that the smart PEGylated polyplexes can indeed penetrate into the multiple cell layers of 3D tumor masses in vivo, exerting therapeutic effects through the RNAi.     

Visualization technique for time-variant hydrogen concentration distribution in porous materials using hydrogen storage alloy thin film

An understanding of the mechanism whereby hydrogen is transported in porous materials is a key factor when developing hydrogen-utilizing equipment. For instance, studying the start-up and degradation characteristics of Polymer Electrolyte Fuel Cell (PEFC) requires the distribution of the time-variant hydrogen concentration to be measured at times of the order of 10^?1 s. However, few studies on time-variant hydrogen transport in porous materials have been reported, and conventional measuring methods are insufficient in terms of their time resolution. This study led to the development of a visualization technique using the characteristics of a MgNi thin film, the reflection rate of which changes along with the hydrogen concentration. The new visualization technique makes it possible to investigate and understand the distribution of the time-variant hydrogen concentration.     

SEM observation of wet biological specimens pretreated with room-temperature ionic liquid

A facile pretreatment process for SEM: The use of room temperature ionic liquids (RTILs) provides an interesting method for SEM of biological specimens. We used a novel and concise method of pretreatment, excluding fixation or Au sputtering steps. Fine and smooth-textured SEM images of a wide variety of biological specimens treated in this way were observed without artefacts.     

A 31P-NMR study of peroxo species formed during oxidation of cyclohexene with hydrogen peroxide in tri-n-butylphosphate catalyzed by heteropolyacids

In the oxidation of cyclohexene with H₂O₂in monophasic tri-n-butylphosphate (TBP) solution catalyzed by Keggin-type 12-heteropolyacids, i.e., H₃PMo_12-xW _x O₄₀(x=0–12), several peroxo species were observed by ³¹P-NMR spectroscopy in lower field than the original heteropolyacids. Their composition varied regularly with that of the starting catalyst. The P-containing peroxo species formed was deduced as [PM₄O₈(O₂)₈]^3-(M = Mo, W). The peroxo species formed more easily with a decrease in the W content, x of H₃PMo_12-xW_xO₄₀. It was further indicated from the reactivity with cyclohexene and the comparison with catalytic performance that W-rich peroxo species were catalytically more active than Mo-rich peroxo species for the oxidation of cyclohexene in this reaction system.     

Investigation of soil deformation characteristics during pullout of a ribbed reinforcement using X-ray micro CT

Steel-strip reinforced earth walls stabilize through the pullout resistance of the reinforcements. Soil dilation during the pullout of ribbed reinforcements may contribute to the evolution of pullout resistance; however, few studies have clarified this mechanism by investigating how soils behave with increasing pullout displacement. The ribs of the reinforcements enhance the pullout resistance, although the influence of the rib dimensions on the evolution of pullout resistance with increasing pullout displacement has not been sufficiently revealed. In the present study, a triaxial pullout apparatus is developed and pullout tests are conducted using ribbed reinforcements with different rib-inclination angles under isotropic stress. The displacement and strain fields in the soils during the pullout of the reinforcements are investigated by X-ray micro CT and a digital image correlation technique. It is found that larger rib-inclination angles provide higher pullout resistance at an early stage of the pullout because of the higher bearing resistance related to the more significant soil densification above the ribs. With increasing pullout displacement, the reinforcements with different rib-inclination angles come to behave as almost one in the same since a rigid soil wedge related to the passive soil failure is generated above the ribs. This tendency results in similar soil deformation characteristics and pullout resistance levels for every reinforcement beyond the soil failure state, although the rib-inclination angles are different.     

Preparation of microporous membranes from cation exchange membranes prepared by the paste method

A microporous membrane is prepared by treating the cation exchange membrane (Fe⁺⁺⁺ form) with an H₂O₂ aqueous solution. The cation exchange membrane is prepared by the paste method: the base membrane is prepared beforehand and then sulfonated. The preparative conditions of the base membrane were studied in connection with the characteristics of the resultant microporous membrane. Furthermore, the availability of the microporous membrane for ultrafiltration was studied by using an aqueous solution of a bovine hemoglobin.     

Heat Transfer Characteristics of Gas-Insulated Transmission Lines

This paper reports on an investigation of the heat transfer characteristics of GILs under constant temperatures and under changing temperatures influenced by solar radiation and wind. It was verified that values calculated by our heat transfer formulas coincided well with measured values both for current-induced temperature rise of the conductor and enclosure and for temperature changes caused by solar radiation and wind. In addition, the influence of installation angle on conductor and enclosure temperature rises was investigated, and it was confirmed that the highest local temperature rises of a conductor and an enclosure above ambient temperature generally do not exceed the temperature rises of horizontal installation, although heat spots existed in tests.     

Probabilistic reconstruction of ancestral protein sequences

Using a maximum-likelihood formalism, we have developed a method with which to reconstruct the sequences of ancestral proteins. Our approach allows the calculation of not only the most probable ancestral sequence but also of the probability of any amino acid at any given node in the evolutionary tree. Because we consider evolution on the amino acid level, we are better able to include effects of evolutionary pressure and take advantage of structural information about the protein through the use of mutation matrices that depend on secondary structure and surface accessibility. The computational complexity of this method scales linearly with the number of homologous proteins used to reconstruct the ancestral sequence.     

Reaction of Silane with Atomic Oxygen at High Temperatures

The reaction of silane with atomic oxygen (³P) was investigated by the shock-tube–laser-photolysis method over the temperature range of 900–1170 K. Oxygen atoms were produced by the ArF laser photolysis of SO₂ behind reflected shock waves and monitored with atomic resonance absorption spectroscopy. The rate constant for the SiH₄ + O reaction was evaluated by taking the possible contribution of the consecutive reaction into consideration. The Arrhenius temperature coefficient was determined to be E_a= 26.6 kJ mol⁻¹, which is much higher than the experimental activation energies obtained at lower temperatures. Rate constants calculated by a transition state theory with the reaction barrier height of E₀= 10.2 kJ mol⁻¹ agreed well with both the present and the previous experimental results for a wide temperature range.