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141.
Watanabe M Fujioka K Akiyama N Takeyama H Manabe N Yamamoto K Manome Y 《IEEE transactions on nanobioscience》2011,10(1):30-35
Among the immunoglobulins, IgM class-antibodies are now considered to be potent immunological reagents for anticancer remedies. However, only a few reports are available about the effective labeling of IgM with enzymes, fluorescence, or other bioreactive reagents. Here, we report an effective application of luminescent semiconductive nanoparticles, quantum dots (QDs), as a labeling material of the IgM antibody. The CdSe carboxyl QDs were reacted with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfo- succinimide in 2-(morpholino) ethanesulfonic acid. The reacted QDs were then coupled to JT95 IgM antibody, which recognizes thyroid carcinoma associated antigen. The specificity and activity of the conjugates were tested by immunoblot, immunoquantitive assay and immunohistological imaging. The QDs were firmly conjugated with JT95 IgM monoclonal antibody. In immunoblot assay, QD-JT95 conjugates directly detected the target molecules without obstructing the binding site. In immunoquantitive assay, the conjugates could quantify the antigen in the range of 1.56-100 μg/mL. Also, QDs-labeled antibody detected the antigen on plasma membrane. Our results demonstrate that labeling of JT95 and other IgM class antibodies with QDs is feasible. This approach may be an important method for the medical application of IgM in the diagnosis and treatment of cancers. 相似文献
142.
Zhou D. Sun K. Liu Z. Huang L. Matsuse K. Sasagawa K. 《Industry Applications, IEEE Transactions on》2007,43(1):3-13
A novel drive and protection circuit for reverse-blocking insulated gate bipolar transistor (RB-IGBT) is proposed in this paper. For the drive circuit, a dynamic current source is introduced to reduce the turn-on and turn-off transients. Meanwhile, the di/dt of the collector current and the dv/dt of the collector-emitter voltage are strictly restricted, so do the respective stresses. The drive circuit consists of a conventional push-pull driver and two controllable current sources-a current generator and a current sink. These two current sources work in switching transitions. For the protection circuit, a novel collector current detecting circuit suitable for RB-IGBT is proposed. This method detects the collector current by sensing collector-emitter voltage of the device. Further study shows that this method can be used to acquire the current signs in commutation transitions of matrix converter. A series of experiments has been carried out concerning the proposed drive and protection circuit and the experimental setup; results as well as detailed analysis are presented 相似文献
143.
Hiroi Sei Kouki Oka Hikaru Sotome Hiroshi Miyasaka Norimitsu Tohnai 《Small (Weinheim an der Bergstrasse, Germany)》2023,19(36):2301887
Expression of room-temperature phosphorescence (RTP) in organic materials requires complicated molecular design and specific intermolecular interactions, and therefore types of RTP materials are restricted. This work presents cage-like sodalite-type porous organic salts (s-POSs) as host materials for luminescent molecules to induce RTP, using tetrasulfonic acid with an adamantane core and triphenylmethylamines that are modified with substituents in the para-positions of benzene rings (TPMA-X). By adding a representative luminescent molecule (pyrene) to a reaction solution during construction of s-POSs, the molecule is incorporated in a facile manner. s-POSs with a heavy halogen atom (X: Iodine) on the pore surface give heavy atom effects, suppression of thermal vibration, and protection from oxygen, for the incorporated molecule, which induce its RTP even in air. This strategy can be applied to various luminescent molecules, which may lead to the achievement of RTP of various colors. 相似文献