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Chen Yanni Liu Liqiang Xu Liguang Song Shanshan Kuang Hua Cui Gang Xu Chuanlai 《Nano Research》2017,10(8):2833-2844
A gold immunochromatographic sensor (GICS) was developed for the rapid detection of 26 sulfonamides in honey samples.The sensor was based on a group-specific monoclonal antibody (mAb) that can recognize all 26 sulfonamides.Three haptens (hapten 1 with a thiazole ring,hapten 2 with a benzene ring,and hapten 3 with a straight carbon chain) were used for antigen preparation.With hybridoma technology,a group-specific mAb was screened with a 50% maximal inhibitory concentration (IC50) against sulfathizole (STZ) and the other 25 analogues ranging from 0.08 to 90.18 ng/mL.Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip.A labeled antibody concentration of 0.1 μg/mL and a coating antigen concentration of 0.2 μg/mL in the test line were chosen for strip preparation.Under optimized conditions,the visual limits of detection (vLOD) for the concentrations of STZ,sulfamethoxazole,sulfamethizole,sulfadiazine,sulfamerazine,sulfadimethoxine,sulfamonomethoxine,sulfameter,sulfamethoxypyridazine,and sulfachloropyridazine were 5,0.25,0.25,10,5,10,25,2.5,5,0.25,and 10 μg/kg,respectively.Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides.Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography.The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples. 相似文献
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Juan Peng Liqiang Liu Liguang Xu Shanshan Song Hua Kuang Gang Cui Chuanlai Xu 《Nano Research》2017,10(1):108-120
For rapid and simultaneous detection of (fluoro)quinolones, a broadly specific monoclonal antibody (mAb) that recognizes 32 (fluoro)quinolone antibiotics was prepared using a mixture of a norfloxacin derivative and a sarfloxacin derivative as the hapten. An immunochromatographic strip based on gold nanoparticles (AuNPs) was then assembled with goat anti-mouse antibody and antigen (sarfloxacin coupled to ovalbumin), used to form the C line and T line, respectively. This antigen competes with the (fluoro)quinolones in a sample incubated with mAbs labeled with AuNPs. The strip can detect 32 (fluoro)quinolones including oxolinic acid, nalidixic acid, miloxacin, pipemidic acid, piromidic acid, rosoxacin, cinoxacin, norfloxacin, pefloxacin, lomfloxacin, enofloxacin, fleroxacin, ciprofloxacin, enrofloxacin, dafloxacin, orbifloxacin, sparfloxacin, gemifloxacin, besifloxacin, balofloxacin, gatifloxacin, moxifloxacin, nadifloxacin, ofloxacin, marbofloxacin, flumequine, pazufloxacin, prulifloxacin, sarafloxacin, difloxacin, trovafloxacin, and tosufloxacin in milk within 10 min with the naked eye. The cut-off values of the strip range from 1 to 100 ng/mL and the limits of detection are 0.1–10 ng/mL. The strip does not cross-react with antibiotics including tetracycline, sulfamethazine, ampicillin, erythromycin, aflatoxin B1, or gentamicin. In short, this immunochromatographic strip is a very useful tool for the primary screening of (fluoro)quinolones in milk. 相似文献
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Highly Efficient Perovskite Nanocrystal Light‐Emitting Diodes Enabled by a Universal Crosslinking Method 下载免费PDF全文
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Phototherapy: Hierarchical Plasmonic Nanorods and Upconversion Core–Satellite Nanoassemblies for Multimodal Imaging‐Guided Combination Phototherapy (Adv. Mater. 5/2016) 下载免费PDF全文
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Sen Zeng Guoqi Ni Xiaojun Kuang 《Journal of Materials Science: Materials in Electronics》2016,27(10):10078-10081
High-Q dielectric materials ilmenite MgTiO3, columbite MgNb2O6 and cubic perovskite Ba3NiTa2O9 with negative temperature coefficient of resonant frequency (τ f ) were selected as candidates for compensating the τ f of hexagonal perovskite Ba8ZnTa6O24. X-ray diffraction data shows that Ba8ZnTa6O24 coexists with Ba3NiTa2O9 but is not compatible with MgTiO3 and MgNb2O6 at high temperature. The τ f for the mixed hexagonal/cubic perovskite Ba8ZnTa6O24–Ba3NiTa2O9 system is tunable via the temperature compensation effect and its quality factor may be improved via annealing the ceramics at high temperature to enhance the cation ordering in the cubic component. Permittivity ε r ~ 22–25, Q×f > 30,000 GHz and tunable τ f within ±10 ppm/°C were achieved in the range of about 50–80 wt% Ba3NiTa2O9 for the hexagonal/cubic perovskite composite Ba8ZnTa6O24–Ba3NiTa2O9 ceramics, which is suitable for the application as dielectric resonators and filters. 相似文献