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51.
NI Perrone-Bizzozero TV Isaacson GM Keidan C Eriqat KF Meiri DD Savage AM Allan 《Canadian Metallurgical Quarterly》1998,71(5):2104-2111
Consumption of moderate quantities of ethanol during pregnancy produces deficits in long-term potentiation in the hippocampal formation of adult offspring. Protein kinase C (PKC)-mediated phosphorylation of the presynaptic protein GAP-43 is critical for the induction of long-term potentiation. We tested the hypothesis that this system is affected in fetal alcohol-exposed (FAE) rats by measuring GAP-43 phosphorylation and PKC activity in the hippocampus of adult offspring of rat dams that had consumed one of three diets throughout gestation: (a) a 5% ethanol liquid diet, which produced a maternal blood ethanol concentration of 83 mg/dl (FAE); (b) an isocalorically equivalent 0% ethanol diet (pair-fed); or (c) lab chow ad libitum. Western blot analysis using specific antibodies to PKC-phosphorylated GAP-43 revealed that FAE rats had an approximately 50% reduction in the proportion of phosphorylated GAP-43. Similarly, we found that PKC-mediated incorporation of 32P into GAP-43 was reduced by 85% in hippocampal slices from FAE rats compared with both control groups. FAE animals also showed a 50% reduction in total hippocampal PKC activity, whereas the levels of six major PKC isozymes did not change in any of the diet groups. These results suggest that GAP-43 phosphorylation deficits in rats prenatally exposed to moderate levels of ethanol are not due to alterations in the expression of either the enzyme or substrate protein, but rather to a defect in kinase activation. 相似文献
52.
Marjorie Ellin Doyle Kathleen A. Glass 《Comprehensive Reviews in Food Science and Food Safety》2010,9(1):44-56
ABSTRACT: Sodium is an essential nutrient with important functions in regulating extracellular fluid volume and the active transport of molecules across cell membranes. However, recent estimates from NHANES III (Third National Health and Nutrition Examination Survey) data show that over 95% of men and over 75% of women exceed the recommended daily tolerable upper intake of sodium. Since these high levels of dietary sodium are associated with a high prevalence of hypertension, prehypertension and, possibly, other adverse effects on health, many national and international health organizations recommend that sodium intake be significantly decreased. Traditionally, salt (sodium chloride) has been used as a food preservative that kills or limits the growth of foodborne pathogens and spoilage organisms by decreasing water activity. Salt also performs other important functions in foods by adding flavor and masking bitter tastes, controlling growth of yeast and fermentative bacteria, and promoting binding of proteins and other components in foods to achieve desired textures. Many processed foods contain high levels of salt and several countries have developed national programs for significantly reducing the sodium chloride content in many processed foods and encouraging a decrease in discretionary salt use. This review considers published data on the apparent adverse health effects of excess salt intake as well as the important functions of salt in different foods and possible strategies for reducing sodium levels in processed foods while still producing safe foods that consumers find acceptable. 相似文献
53.
Luca Pezzato Leonardo Bertolucci Coelho Rachele Bertolini Alessio Giorgio Settimi Katya Brunelli Marjorie Olivier Manuele Dabal 《工业材料与腐蚀》2019,70(11):2103-2112
Plasma electrolytic oxidation (PEO) coatings were produced on AZ80 magnesium alloy in a solution containing silicates and phosphates and working at high current densities with short treatment times. The effect of a sealing treatment in boiling water on corrosion and mechanical properties of the coatings were investigated. Moreover, the corrosion mechanism of the samples with and without the sealing treatment was evaluated. The microstructure of the coatings was characterized with scanning electron microscope observation and X‐ray diffraction analysis. The mechanical properties were evaluated with nanoindentation tests and the corrosion resistance was studied by potentiodynamic polarization, electrochemical impedance spectroscopy, and scanning vibrating electrode technique. The results showed that the sealing did not influence the microstructure and the mechanical properties of the samples and instead produced a remarkable increase in the corrosion resistance. The crevice corrosion, present in the sample without the sealing, was avoided with the treatment in boiling water. 相似文献
54.
Metal concentrations in rural topsoil in South Carolina: potential for human health impact 总被引:11,自引:0,他引:11
Aelion CM Davis HT McDermott S Lawson AB 《The Science of the total environment》2008,402(2-3):149-156
Rural areas are often considered to have relatively uncontaminated soils; however few studies have measured metals in surface soil from low population areas. Many metals, i.e., arsenic (As), lead (Pb), and mercury (Hg), have well-documented negative neurological effects, and the developing fetus and young children are particularly at risk. Using a Medicaid database, two areas were identified: one with no increased prevalence of mental retardation and developmental delay (MR/DD) (Strip 1) and one with significantly higher prevalence of MR/DD (Strip 2) in children compared to the state-wide average. These areas were mapped and surface soil samples were collected from 0-5 cm depths from nodes of a uniform grid laid out across the sampling areas. Samples were analyzed for As, barium (Ba), beryllium (Be), chromium (Cr), copper (Cu), Pb, manganese (Mn), nickel (Ni), and Hg. Inverse distance weighting (IDW) was used to estimate concentrations throughout each strip area, and a principal component analysis (PCA) was used to identify common sources. All metal concentrations in Strip 2, the MR/DD cluster area, were significantly greater than those in Strip 1 and similar to those found in more urban and highly agricultural areas. Both Strips 1 and 2 had a high number of significant correlations between metals (33 for Strip 1 and 25 for Strip 2), suggesting possible similar natural or anthropogenic sources which was corroborated by PCA. While exposures were not assessed and direct causation between environmental soil metal concentrations and MR/DD cannot be concluded, the high metal concentrations in areas with an elevated prevalence of MR/DD warrants further consideration. 相似文献
55.
Electrical impedance tomography (EIT) is a badly posed inverse problem, but can be stabilized if one assumes that the conductivity is piecewise constant, with a relatively small number of distinct regions, and that the region boundaries are known, for example from prior anatomical imaging. With this assumption, we introduce a three-dimensional (3-D) boundary element method (BEM) model for the forward EIT map from injected currents to measured voltages, and 3-D inverse solutions for both BEM and the finite element method (FEM) which explicitly take into account the parameterization implied by the known boundary locations. We develop expressions for the Jacobians for both methods, since they are nonlinear, to more rapidly solve the inverse problem. We show simulation results in a torso geometry with the heart and lungs as inhomogeneities. In a simulation study, we could reconstruct the conductive values of some internal organs of a human torso with more than 92% accuracy even with inaccurate internal boundary locations, a randomized rather than constant conductivity profile (with the standard deviation of the Gaussian-distributed conductivities set to 20% of their mean values), signal to measurement noise of 50 dB, and with different meshes used for the forward and inverse problems. BEM and FEM perform similarly, leading to the conclusion that the choice between them should be based on secondary considerations such as computational efficiency or the need to model conductivity anisotropies 相似文献
56.
Marjorie S. Austero Amalie E. Donius Ulrike G. K. Wegst Caroline L. Schauer 《Journal of the Royal Society Interface》2012,9(75):2551-2562
Chitosan (CS), the deacetylated form of chitin, the second most abundant, natural polysaccharide, is attractive for applications in the biomedical field because of its biocompatibility and resorption rates, which are higher than chitin. Crosslinking improves chemical and mechanical stability of CS. Here, we report the successful utilization of a new set of crosslinkers for electrospun CS. Genipin, hexamethylene-1,6-diaminocarboxysulphonate (HDACS) and epichlorohydrin (ECH) have not been previously explored for crosslinking of electrospun CS. In this first part of a two-part publication, we report the morphology, determined by field emission scanning electron microscopy (FESEM), and chemical interactions, determined by Fourier transform infrared microscopy, respectively. FESEM revealed that CS could successfully be electrospun from trifluoroacetic acid with genipin, HDACS and ECH added to the solution. Diameters were 267 ± 199 nm, 644 ± 359 nm and 896 ± 435 nm for CS–genipin, CS–HDACS and CS–ECH, respectively. Short- (15 min) and long-term (72 h) dissolution tests (T600) were performed in acidic, neutral and basic pHs (3, 7 and 12). Post-spinning activation by heat and base to enhance crosslinking of CS–HDACS and CS–ECH decreased the fibre diameters and improved the stability. In the second part of this publication, we report the mechanical properties of the fibres. 相似文献
57.
Yacine Aït Oumeziane Sophie Moissette Marjorie Bart Florence Collet Sylvie Pretot Christophe Lanos 《Journal of Building Performance Simulation》2017,10(3):256-271
Building envelopes with bio-materials like hemp concrete are currently undergoing an increasing development due to their low environmental impact and their interesting hygrothermal properties. This kind of hygroscopic material is characterized by a hysteretic behaviour. In this paper, the influence of such behaviour on the hygrothermal response of a building wall is discussed. An experimental facility is designed to measure temperature and relative humidity within a hemp concrete wall submitted to climatic variations. This facility provides a set of experimental data suited for benchmarking. Numerical simulations are performed with a hysteresis model implemented in a heat and moisture transfer model. Comparisons between numerical and experimental results show that hysteresis modelling is relevant to simulate the hygrothermal response of the wall. The discussion is extended to the influence of the initial moisture content. 相似文献
58.
59.
Increasing production and use of fullerene-based nanomaterials underscore the need to determine their mobility in environmental transport pathways and potential ecological exposures. This study investigated the transport of two fullerenes (i.e., aqu/C60 and water-soluble C60 pyrrolidine tris-acid [C60 PTA]) in columns packed with model porous media (Iota quartz and Ottawa sand) and a sediment from Call’s creek under saturated and unsaturated steady-state flows. The fullerenes had the least retention in Iota quartz, and the greatest retention in the sediment at near neutral pH, correlating with the degree of grain surface chemical heterogeneity (e.g., amorphous Al hydroxides concentration increasing in the order of Iota quartz < Ottawa sand < sediment). Surface roughness was elucidated as another important factor responsible for the greatest fullerene retention in the sediment. In accordance with the XDLVO energy calculations, C60 PTA was less retained than aqu/C60 at near neutral pH, due to its greater hydrophilicity measured by tolune-water partition coefficient, as well as smaller particle sizes revealed by atomic force microscopy. Fullerene retention exhibited a strong dependency on solution pH that could be explained partly by the pH-dependent surface charge of fullerenes and grain surface, and partly by increased hydrophobicity of C60 PTA when solution pH approaches its isoelectric point (IEP). Finally, fullerene retention was enhanced in unsaturated media, implying that fullerenes may be more attenuated in the vadose zone than in groundwater. 相似文献
60.
Jie Kang Keshari M. Thakali Chenghui Xie Miwako Kondo Yudong Tong Boxin Ou Gitte Jensen Marjorie B. Medina Alexander G. Schauss Xianli Wu 《Food chemistry》2012
There are two predominant palm tree species producing edible fruit known as “açaí” found widely dispersed through the Amazon: Euterpe oleracea Mart. and Euterpe precatoria Mart. They differ from each other in terms of how the plants grow and their phytochemical composition. E. oleracea (EO) has received considerable attention as a “super fruit” because of its high antioxidant capacity, while studies on E. precatoria (EP) remain rare. In this study, the antioxidant and anti-inflammatory activities of EP fruit pulps were evaluated by different assays including a series of oxygen radical absorbance capacity (ORAC) based assays, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the cell-based antioxidant protection in erythrocyte (CAP-e) assay, as well as the nuclear factor-kappa B (NF-κB) secreted embryonic alkaline phosphatase (SEAP) assay. Total phenolics were also measured as an indication of the total phenol content. For comparative purposes, the EO fruit pulp was included. The antioxidant capacity of the EP fruit pulp was determined to be superior to the EO fruit pulp in every chemical based assay. In the cell-based CAP-e assay, the EP fruit pulp showed a dose-dependent inhibition against oxidative damage with an IC50 of 0.167 g/l. In the SEAP reporter assay, the EP fruit pulp polyphenol-rich extracts inhibited lipopolysaccharide (LPS)-induced NF-κB activation by 23% (p < 0.05) at 20 μg/ml, whereas the extract of the EO fruit pulp did not show a significant inhibitory effect at comparable doses. In addition, carotenoids were quantified for the first time in EP, since EP has high scavenging capacity against singlet oxygen. 相似文献