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31.
Machida Y Hattori K Miyake K Kawase Y Kawase M Iijima S 《Journal of Bioscience and Bioengineering》2000,90(1):62-68
Bacteriophage 63D, previously isolated from sewage, is associated with alpha-2,8-linked polysialic acid degrading activity. We cloned a DNA fragment containing the sialidase gene from a 63D phage genomic library and the enzyme was functionally expressed in Escherichia coli. Determination of the nucleotide sequence of the fragment revealed that it contained one open reading frame (ORF) coding for a 108-kDa polypeptide consisting of 984 amino acid residues. The fragment had promoter sequences similar to the E. coli consensus promoters for sigma70. The deduced amino acid sequence of the central region of the ORF showed homology to those of phages K1F (51.6% identity) and PK1E (51.7% identity) endosialidases. Two Asp-box motifs that are widely found in sialidases were conserved. Purification of the soluble enzyme from lysed culture broth of infected E. coli yielded a 90-kDa protein upon SDS polyacrylamide gel electrophoresis, suggesting that the primary translational product is processed to the mature 90-kDa protein. The molecular mass of the enzyme was determined as 360 kDa by gel filtration, indicating that the native enzyme was probably a tetramer of identical 90-kDa subunits. 相似文献
32.
Abstract. This paper deals with the asymptotic efficiency of the sample autocovariances of a Gaussian stationary process. The asymptotic variance of the sample autocovariances and the Cramer–Rao bound are expressed as the integrals of the spectral density and its derivative. We say that the sample autocovariances are asymptotically efficient if the asymptotic variance and the Cramer–Rao bound are identical. In terms of the spectral density we give a necessary and sufficient condition that they are asymptotically efficient. This condition is easy to check for various spectra. 相似文献
33.
Film-type electrodes of hydrogen absorbing intermetallic compound and alloys, LaNi5, Ni0.11 Ti0.89, Ni0.50 Ti0.50 and Ni0.76 Ti0.24 were prepared by a flash evaporation method. The hydrogen electrode reaction characteristics of the LaNi5 and NiTi alloy films in 1 M NaOH are very similar to each other. The reaction proceeds via the Volmer-Tafel reaction route with mixed rate-determining characteristics. The exchange current densities of the constituent steps, as well as the overall reaction, are in the range of 10?6 A cm?2 (true). Surface analysis by an XPS technique has shown that La or Ti on the electrode surface exists as an electrocatalytically innert oxide of La2O3 or TiO2. Close similarities of these electrodes with pure Ni electrodes indicate that Ni is responsible for the electrocatalytic activity. No synergistic effect is thus noticeable. 相似文献
34.
AC Lidral PA Romitti AM Basart T Doetschman NJ Leysens S Daack-Hirsch EV Semina LR Johnson J Machida A Burds TJ Parnell JL Rubenstein JC Murray 《Canadian Metallurgical Quarterly》1998,63(2):557-568
Nonsyndromic cleft lip with or without cleft palate (CL/P) and nonsyndromic cleft palate only (CPO) are common congenital anomalies with significant medical, psychological, social, and economic ramifications. Both CL/P and CPO are examples of complex genetic traits. There exists sufficient evidence to hypothesize that disease loci for CL/P and CPO can be identified by a candidate-gene linkage-disequilibrium (LD) strategy. Candidate genes for clefting, including TGFA, BCL3, DLX2, MSX1, and TGFB3, were screened for LD with either CL/P or CPO in a predominantly Caucasian population, with both case-control- and nuclear-family-based approaches. Previously reported LD for TGFA with both CL/P and CPO could not be confirmed, except in CL/P patients with a positive family history. Also, in contrast to previous studies, no LD was found between BCL3 and either CL/P or CPO. Significant LD was found between CL/P and both MSX1 and TGFB3 and between CPO and MSX1, suggesting that these genes are involved in the pathogenesis of clefting. In addition, a mutation search in the genes DLX2, MSX1, and TGFB3 was performed in 69 CPO patients and in a subset of the CL/P patients. No common mutations were found in the coding regions of these genes; however, several rare variants of MSX1 and TGFB3 were found that may alter the latters' normal function. These results form the basis for future research, including (a) mutation searches in the MSX1 and TGFB3 genes in Caucasian CL/P patients and (b) extension of the search for MSX1 mutations in CPO patients to the noncoding regions. 相似文献
35.
36.
ABSTRACT Parametric analyses of the strain-stress in the drying process are performed to study the influence of the intermittent drying on the behaviors of drying-induced strain-stress and deformation as well as the drying characteristic. The transient three-dimensional problem of strain-stress and heat and moisture transfer in a slab is solved simultaneously by the finite element method. The dimensionless parameters are introduced to generalize the problem in the conservation equations of heat and moisture transfer. The intermittent drying is modeled by applying periodically smaller and larger Biot numbers to the boundary conditions. The maximum tensile and compressive stresses fluctuate, and fall remarkably during the smaller Biot number period when a slab is heated intermittently. The peak stress of the fluctuation exceeds beyond the case in the continuous healing where the overall drying rate is almost equivalent to that in the intermittent beating, but the reduction of the stresses takes place rapidly in the low heating period. 相似文献
37.
The equilibrium adsorption of acid dyes on acrylic fibres copolymerised with dimethyl amino ethyl methacrylate has been investigated. A typical characteristic of this type of fibre is its excellent stable dyeability over a wide pH range. This is due to the strong basicity of alkylamino groups in the copolymerised component, which makes it possible to dye the fibre thoroughly even in a weakly acidic dyebath using a normal dyeing processes of acrylic fibre with cationic dye. Consequently it is useful for differential cross-dyeing in a single dyebath. 相似文献
38.
Norimasa Nukaga Hirofumi Ono Takuya Shida Hideaki Machida Toshie Suzuki Hiroshi Funakubo 《组合铁电体》2013,141(1):215-222
SrBi 2 Ta 2 O 9 thin films were prepared by ECR plasma enhanced metalorganic chemical vapor deposition (ECR-MOCVD) with a liquid-delivery system using one cocktail source without an additional solvent. The strontium-tantalum double alkoxide, Sr{Ta[OC 2 H 4 H(CH 3 ) 2 ](OC 2 H 5 ) 5 } 2 , was dissolved in stabilized trimethyl bismuth, Bi(CH 3 ) 3 / dioxane. This source system has been used in a conventional bubbling system. Deposition rate and the composition of the films were strictly controlled by the concentration and the composition of the cocktail source. Therefore, high reproducibility was realized by using this system. The constituent phase of the stoichiometric SBT film as-deposited at 500 C on a (111)Pt/TiO 2 /SiO 2 /Si substrate was a fluorite phase and transformed to the single phase of SBT by the post annealing at 800 C. It showed almost the same ferroelectricity as the stoichiometric composition film. 相似文献
39.
Naoki Uchida Yoshie Maitani Yoshiharu Machida Masayuki Nakagaki Tsuneji Nagai 《Drug development and industrial pharmacy》1991,17(12):1625-1634
The nasal drug absorption and the effect of absorption promoters have been studied in rabbits. Nasal mucosa excised from rabbits was mounted as a flat sheet in an in vitro chamber. The result indicates that the change in the porosity of the membrane by pretreatment with bile salts increased the permeability coefficient of sodium chloride in the nasal membrane. The permeabilities of dextran derivatives were enhanced by pretreatment with sodium glycocholate (GC). The permeability coefficient (P) of fluorescein isothiocyanate diethylaminoethyl dextran 相似文献
40.
A. Trigueiros M. Machida C. J. B. Pagan
J. G. Reyna-Almandos
《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》1989,280(2-3):589-592Preliminary results of the analysis of the spectra of four times ionized krypton, Kr-V, are presented. 28 transitions were classified and for five of them the classification is new. We also present the UNICAMP theta-pinch for the study of highly ionized atoms. This device is now in testing. 相似文献