全文获取类型
收费全文 | 355篇 |
免费 | 9篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 43篇 |
化学工业 | 49篇 |
金属工艺 | 7篇 |
机械仪表 | 12篇 |
建筑科学 | 6篇 |
能源动力 | 8篇 |
轻工业 | 13篇 |
无线电 | 47篇 |
一般工业技术 | 87篇 |
冶金工业 | 68篇 |
原子能技术 | 11篇 |
自动化技术 | 14篇 |
出版年
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 4篇 |
2019年 | 2篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2016年 | 4篇 |
2015年 | 4篇 |
2014年 | 6篇 |
2013年 | 10篇 |
2012年 | 10篇 |
2011年 | 12篇 |
2010年 | 5篇 |
2009年 | 6篇 |
2008年 | 18篇 |
2007年 | 11篇 |
2006年 | 13篇 |
2005年 | 15篇 |
2004年 | 9篇 |
2003年 | 11篇 |
2002年 | 11篇 |
2001年 | 8篇 |
2000年 | 8篇 |
1999年 | 15篇 |
1998年 | 30篇 |
1997年 | 20篇 |
1996年 | 19篇 |
1995年 | 8篇 |
1994年 | 10篇 |
1993年 | 11篇 |
1992年 | 8篇 |
1991年 | 11篇 |
1990年 | 6篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 1篇 |
排序方式: 共有365条查询结果,搜索用时 15 毫秒
61.
Sundararajan N Mao D Chan S Koo TW Su X Sun L Zhang J Sung KB Yamakawa M Gafken PR Randolph T McLerran D Feng Z Berlin AA Roth MB 《Analytical chemistry》2006,78(11):3543-3550
Posttranslational modification (PTM) of proteins is likely to be the most common mechanism of altering the expression of genetic information. It is essential to characterize PTMs to establish a complete understanding of the activities of proteins. Here, we present a sensitive detection method using surface-enhanced Raman spectroscopy (SERS) that can detect PTMs from as little as zeptomoles of peptide. We demonstrate, using model peptides, the ability of SERS to detect a variety of protein modifications, such as acetylation, trimethylation, phosphorylation, and ubiquitination. In addition, we show the capability to obtain positional information for modifications such as trimethylation and phosphorylation using SERS and wavelet decomposition data analysis techniques. We further show that it is possible to apply SERS to detect PTMs from biological samples such as histones. We envision that this detection method might be a valuable technique that is complementary to mass spectrometry in obtaining orthogonal chemical and modification-specific information from biological samples at sensitive levels. 相似文献
62.
A method for fabricating epoxy resin films dispersing the surface‐modified barium titanate (BT) particles (BT‐epoxy resin composite films) are proposed. BT particles with a size of 7.8 nm and a crystal size of 8.6 nm were synthesized with a complex alkoxide method. To introduce epoxy groups on the BT particle surface, the BT particles were surface‐modified with 2‐(3,4‐epoxycyclohexyl)‐ethyltrimethoxysilane. A precursor solution, which was prepared by prereacting 2,2‐bis(4‐glycidyloxyphenyl)propane (BGPP) and phthalic anhydride in 4‐butyrolactone and adding the surface‐modified BT particles to the prereacting solution, was spin‐coated on glass substrates to fabricate the composite films. An increase in BT volume fraction in film increased dielectric constant of the composite film while keeping dissipation factor below 0.03. The dielectric constant attained 10.8 at a BT volume fraction of 30% in film that was around twice higher than pure epoxy resin film. POLYM. COMPOS., 31:1179–1183, 2010. © 2009 Society of Plastics Engineers 相似文献
63.
N. Momono T. Kurosawa Y. Amakai Y. Sato T. Suzuki H. Takano S. Murayama A. Sakai M. Oda M. Ido 《Journal of Superconductivity and Novel Magnetism》2010,23(5):785-787
The Raman scattering experiments were carried out in Zn-doped and Zn-free Bi2Sr2CaCu2O8+y
with optimal hole concentration below and above T
c
. The energy of pair-breaking peak in the B
1g
Raman spectrum, corresponding to the magnitude of superconducting gap 2Δ
0, is suppressed by 1% Zn-doping. In the normal state, the B
1g
Raman spectrum for Zn-doped sample shows no pseudogap behaviour, suggesting that the pseudogap is strongly smeared by Zn-doping. 相似文献
64.
Kanazawa T Atsumi M Mineo H Fukushima M Nishimura N Noda T Chiji H 《Journal of oleo science》2008,57(6):335-343
Potato starch is known to have a higher concentration of phosphate than other starches. The presence of phosphate groups in amylopectin results in resistance to digestion by amylase. Therefore, there is a possibility that potato starch is slowly digested, inducing a physiological effect similar to that of resistant starch and indigestible oligosaccharides. The amount of phosphate group in starch differs with potato cultivar. In the present study, we investigated the effects of gelatinized potato starch containing a high level of phosphorus on lipid metabolism in rats. For this purpose, we determined lipid levels in the serum and liver in rats fed two kinds of gelatinized potato starches with different phosphorus contents. Four groups of male Sprague-Dawley rats were fed a 60% sucrose diet (control) or one of three diets containing cornstarch (CS), Benimaru (BM) potato starch or Hokkaikogane (HK) potato starch. Fat pad weight was slightly decreased in the HK diet group compared with that in the other groups. Free fatty acids in serum were significantly lowered by dietary HK starch compared with control, and serum triglyceride in rats fed the HK diet was also decreased. In the BM and HK diet groups, triglyceride levels in the liver were decreased compared with that in the control and CS groups. As for hepatic total cholesterol level, there were no significant differences among three starch diet groups. Fecal bile acid excretion was greater in the two potato starch groups than in the control group. On the other hand, there were no significant differences in cecal short-chain fatty acid content or pH. Thus, we conclude that dietary gelatinized potato starch reduces free fatty acid and triglyceride in serum and hepatic triglyceride, but does not affect cecal fermentation. 相似文献
65.
Tsuchida S Kawamoto K Nunome K Hamaue N Yoshimura T Aoki T Kurosawa T 《Journal of oleo science》2011,60(5):221-228
Enoyl-coenzyme A (CoA) hydratase catalyzes the hydration of trans-2-enoyl-CoA to yield 3-hydroxyacyl-CoA during fatty acid degradation (β-oxidation). Although much research has focused on the stereospecificities of 2-enoyl-CoA hydratases, a direct quantification of the production of 3(R)- and 3(S)-hydroxyacyl-CoA has not yet been established. Therefore, we developed a method of concurrently quantifying 3(R)- and 3(S)-hydroxyacyl-CoA using high-performance liquid chromatography (HPLC) equipped with a chiral separation column. The optimized conditions for the separation of 3(R)-, 3(S)-hydroxyhexadecanoyl-CoA and trans-2-hexadecenoyl-CoA, were determined to be as follows: mobile phase of 35/65 (v/v) of 50 mM phosphate buffer (pH 5.0)/methanol; flow rate of 0.5 mL/min; detection at 260 nm; and column temperature of 25°C. This method was applied to subcellular fractions of rat liver; the results directly confirmed that 3(S)-hydroxyhexadecanoyl-CoA is the dominant product obtained from the heat-stable enoyl-CoA hydratase-catalyzed reaction of trans-2-hexadecenoyl-CoA. Finally, the stereospecificities of L-bifunctional protein (L-BP) and D-bifunctional protein (D-BP) were reinvestigated using this method, and it was confirmed that L- and D-BP yielded 3(S)- and 3(R)-hydroxyhexadecanoyl-CoA were yielded from trans-2-hexadecenoyl-CoA, respectively. 3(R)-Hydroxyacyl-CoA is a peroxisomal β-oxidation-specific intermediate. Therefore, this method is potentially useful not only studies regarding the stereochemistry of enoyl-CoA hydratase but also for the diagnosis of diseases caused by defects of peroxisomal enoyl-CoA hydratase. 相似文献
66.
Hitoshi Morioka Keisuke Saito Shintaro Yokoyama Takahiro Oikawa Toshiyuki Kurosawa Hiroshi Funakubo 《Journal of Materials Science》2009,44(19):5318-5324
Epitaxial Pb(Zr0.35Ti0.65)O3 (PZT) thin films with tetragonal symmetry and thicknesses ranging from 45 to 230 nm were grown at 540 °C on SrRuO3-coated (001)SrTiO3 substrates by pulse-injected metalorganic chemical vapor deposition. The effect of the film thickness on the ferroelectric
domain structure and the dielectric and ferroelectric properties were systematically investigated. Domain structure analysis
of epitaxial PZT films was accomplished with high-resolution X-ray diffraction reciprocal space mapping and high-resolution
transmission electron microscopy. Fully polar-axis (c-axis)-oriented epitaxial PZT thin films with high ferroelectric polarization values [e.g., remanent polarization (P
r) ~ 90 μC/cm2] were observed for film thicknesses below 70 nm. Films thicker than 70 nm had a c/a/c/a polydomain structure and the relative volume fraction of c-domains monotonously decreased to about 72% on increasing the film thickness up to 230 nm , and finally P
r diminished to about 64 μC/cm2 for the 230-nm-thick epitaxial film. These polarization values were in good agreement with the estimated values taking into
account the volume fraction of the c-axis-oriented domains while assuming a negligible contribution of 90° domain reorientation caused by an externally applied
electric field. 相似文献
67.
基于磁通观察器的感应电机无速度传感器矢量控制系统 总被引:13,自引:1,他引:12
提出一个基于磁通观察器的感应电机无速度传感器矢量控制系统。根据感应电机矢量控制理论 ,我们利用 q轴磁通收敛于零实现转速推算。本系统的优点是系统简单 ,并且避免了利用电压模型所引起的问题。实验结果验证了本调速方案的有效性 相似文献
68.
Bismuth nano-Hall probes with dimensions ~120 × 120 nm were fabricated by focused ion beam milling and used for the direct room temperature magnetic imaging of crystalline garnet thin films and strontium ferrite permanent magnets by scanning Hall probe microscopy. At driving currents of 40 μA, the Hall coefficient and magnetic field sensitivity of the Bi nano-Hall probes were 3.3 × 10-4 Ω/G and 7.2 G/√Hz, respectively 相似文献
69.
Kunal Kumar Olaf Stefanczyk Koji Nakabayashi Yuuki Mineo Shin-ichi Ohkoshi 《International journal of molecular sciences》2022,23(11)
Molecular vibrations in the solid-state, detectable in the terahertz (THz) region, are the subject of research to further develop THz technologies. To observe such vibrations in terahertz time-domain spectroscopy (THz-TDS) and low-frequency (LF) Raman spectroscopy, two supramolecular assemblies with the formula [NdIII (phen)3 (NCX)3] 0.3EtOH (X = S, 1-S; Se, 1-Se) were designed and prepared. Both compounds show several THz-TDS and LF-Raman peaks in the sub-THz range, with the lowest frequencies of 0.65 and 0.59 THz for 1-S and 1-Se, and 0.75 and 0.61 THz for 1-S and 1-Se, respectively. The peak redshift was observed due to the substitution of SCN− by SeCN−. Additionally, temperature-dependent TDS-THz studies showed a thermal blueshift phenomenon, as the peak position shifted to 0.68 THz for 1-S and 0.62 THz for 1-Se at 10 K. Based on ab initio calculations, sub-THz vibrations were ascribed to the swaying of the three thiocyanate/selenocyanate. Moreover, both samples exhibited near-infrared (NIR) emission from Nd (III), and very good thermometric properties in the 300–150 K range, comparable to neodymium (III) oxide-based thermometers and higher than previously reported complexes. Moreover, the temperature dependence of fluorescence and THz spectroscopy analysis showed that the reduction in anharmonic thermal vibrations leads to a significant increase in the intensity and a reduction in the width of the emission and LF absorption peaks. These studies provide the basis for developing new routes to adjust the LF vibrational absorption. 相似文献
70.
Intravenous administration of interleukin-1 (IL-1) activates central autonomic neuronal circuitries originating in the nucleus of the solitary tract (NTS). The mechanism(s) by which blood-borne IL-1 regulates brain functions, whether by operating across the blood-brain barrier and/or by activating peripheral sensory afferents, remains to be characterized. It has been proposed that vagal afferents originating in the periphery may monitor circulating IL-1 levels, because neurons within the NTS are primary recipients of sensory information from the vagus nerve and also exhibit exquisite sensitivity to blood-borne IL-1. In this study, we present evidence that viscerosensory afferents of the vagus nerve respond to intravenously administered IL-1beta. Specific labeling for mRNAs encoding the type 1 IL-1 receptor and the EP3 subtype of the prostaglandin E2 receptor was detected in situ over neuronal cell bodies in the rat nodose ganglion. Moreover, intravenously applied IL-1 increased the number of sensory neurons in the nodose ganglion that express the cellular activation marker c-Fos, which was matched by an increase in discharge activity of vagal afferents arising from gastric compartments. This response to IL-1 administration was attenuated in animals pretreated with the cyclooxygenase inhibitor indomethacin, suggesting partial mediation by prostaglandins. In conclusion, these results demonstrate that somata and/or fibers of sensory neurons of the vagus nerve express receptors to IL-1 and prostaglandin E2 and that circulating IL-1 stimulates vagal sensory activity via both prostaglandin-dependent and -independent mechanisms. 相似文献